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VOC breath profile in spontaneously breathing awake swine during Influenza A infection
Influenza is one of the most common causes of virus diseases worldwide. Virus detection requires determination of Influenza RNA in the upper respiratory tract. Efficient screening is not possible in this way. Analysis of volatile organic compounds (VOCs) in breath holds promise for non-invasive and...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173698/ https://www.ncbi.nlm.nih.gov/pubmed/30291257 http://dx.doi.org/10.1038/s41598-018-33061-2 |
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author | Traxler, Selina Bischoff, Ann-Christin Saß, Radost Trefz, Phillip Gierschner, Peter Brock, Beate Schwaiger, Theresa Karte, Claudia Blohm, Ulrike Schröder, Charlotte Miekisch, Wolfram Schubert, Jochen K. |
author_facet | Traxler, Selina Bischoff, Ann-Christin Saß, Radost Trefz, Phillip Gierschner, Peter Brock, Beate Schwaiger, Theresa Karte, Claudia Blohm, Ulrike Schröder, Charlotte Miekisch, Wolfram Schubert, Jochen K. |
author_sort | Traxler, Selina |
collection | PubMed |
description | Influenza is one of the most common causes of virus diseases worldwide. Virus detection requires determination of Influenza RNA in the upper respiratory tract. Efficient screening is not possible in this way. Analysis of volatile organic compounds (VOCs) in breath holds promise for non-invasive and fast monitoring of disease progression. Breath VOC profiles of 14 (3 controls and 11 infected animals) swine were repeatedly analyzed during a complete infection cycle of Influenza A under high safety conditions. Breath VOCs were pre-concentrated by means of needle trap micro-extraction and analysed by gas chromatography mass spectrometry before infection, during virus presence in the nasal cavity, and after recovery. Six VOCs could be related to disease progression: acetaldehyde, propanal, n-propyl acetate, methyl methacrylate, styrene and 1,1-dipropoxypropane. As early as on day four after inoculation, when animals were tested positive for Influenza A, differentiation between control and infected animals was possible. VOC based information on virus infection could enable early detection of Influenza A. As VOC analysis is completely non-invasive it has potential for large scale screening purposes. In a perspective, breath analysis may offer a novel tool for Influenza monitoring in human medicine, animal health control or border protection. |
format | Online Article Text |
id | pubmed-6173698 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-61736982018-10-09 VOC breath profile in spontaneously breathing awake swine during Influenza A infection Traxler, Selina Bischoff, Ann-Christin Saß, Radost Trefz, Phillip Gierschner, Peter Brock, Beate Schwaiger, Theresa Karte, Claudia Blohm, Ulrike Schröder, Charlotte Miekisch, Wolfram Schubert, Jochen K. Sci Rep Article Influenza is one of the most common causes of virus diseases worldwide. Virus detection requires determination of Influenza RNA in the upper respiratory tract. Efficient screening is not possible in this way. Analysis of volatile organic compounds (VOCs) in breath holds promise for non-invasive and fast monitoring of disease progression. Breath VOC profiles of 14 (3 controls and 11 infected animals) swine were repeatedly analyzed during a complete infection cycle of Influenza A under high safety conditions. Breath VOCs were pre-concentrated by means of needle trap micro-extraction and analysed by gas chromatography mass spectrometry before infection, during virus presence in the nasal cavity, and after recovery. Six VOCs could be related to disease progression: acetaldehyde, propanal, n-propyl acetate, methyl methacrylate, styrene and 1,1-dipropoxypropane. As early as on day four after inoculation, when animals were tested positive for Influenza A, differentiation between control and infected animals was possible. VOC based information on virus infection could enable early detection of Influenza A. As VOC analysis is completely non-invasive it has potential for large scale screening purposes. In a perspective, breath analysis may offer a novel tool for Influenza monitoring in human medicine, animal health control or border protection. Nature Publishing Group UK 2018-10-05 /pmc/articles/PMC6173698/ /pubmed/30291257 http://dx.doi.org/10.1038/s41598-018-33061-2 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Traxler, Selina Bischoff, Ann-Christin Saß, Radost Trefz, Phillip Gierschner, Peter Brock, Beate Schwaiger, Theresa Karte, Claudia Blohm, Ulrike Schröder, Charlotte Miekisch, Wolfram Schubert, Jochen K. VOC breath profile in spontaneously breathing awake swine during Influenza A infection |
title | VOC breath profile in spontaneously breathing awake swine during Influenza A infection |
title_full | VOC breath profile in spontaneously breathing awake swine during Influenza A infection |
title_fullStr | VOC breath profile in spontaneously breathing awake swine during Influenza A infection |
title_full_unstemmed | VOC breath profile in spontaneously breathing awake swine during Influenza A infection |
title_short | VOC breath profile in spontaneously breathing awake swine during Influenza A infection |
title_sort | voc breath profile in spontaneously breathing awake swine during influenza a infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173698/ https://www.ncbi.nlm.nih.gov/pubmed/30291257 http://dx.doi.org/10.1038/s41598-018-33061-2 |
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