Enhancement of PCR Sensitivity and Yield Using Thiol-modified Primers

Various additives can enhance the quality of PCR amplification, but these generally require considerable optimization to achieve peak performance. Here, we demonstrate that the use of thiol-modified primers can enhance both PCR sensitivity and yield. In experiments with V. parahaemolyticus genomic D...

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Detalles Bibliográficos
Autores principales: Bai, Yalong, Xiao, Yi, Suo, Yujuan, Shen, Yuanyuan, Shao, Yi, Zhang, Donglai, Zhou, Changyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173752/
https://www.ncbi.nlm.nih.gov/pubmed/30291287
http://dx.doi.org/10.1038/s41598-018-33223-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173752/
https://www.ncbi.nlm.nih.gov/pubmed/30291287
http://dx.doi.org/10.1038/s41598-018-33223-2

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