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Enhancement of PCR Sensitivity and Yield Using Thiol-modified Primers
Various additives can enhance the quality of PCR amplification, but these generally require considerable optimization to achieve peak performance. Here, we demonstrate that the use of thiol-modified primers can enhance both PCR sensitivity and yield. In experiments with V. parahaemolyticus genomic D...
Autores principales: | Bai, Yalong, Xiao, Yi, Suo, Yujuan, Shen, Yuanyuan, Shao, Yi, Zhang, Donglai, Zhou, Changyan |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173752/ https://www.ncbi.nlm.nih.gov/pubmed/30291287 http://dx.doi.org/10.1038/s41598-018-33223-2 |
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