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Ultrastructural viewpoints on the interaction events of Scedosporium apiospermum conidia with lung and macrophage cells

BACKGROUND: Scedosporium apiospermum is a ubiquitous, emerging and multidrug-resistant fungal pathogen with still rather unknown virulence mechanisms. OBJECTIVES/METHODS: The cellular basis of the in vitro interaction between fungi and host cells/tissues is the determinant factor for the development...

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Autores principales: Aor, Ana Carolina, Mello, Thaís P, Sangenito, Leandro S, Fonseca, Beatriz B, Rozental, Sonia, Lione, Viviane F, Veiga, Venício F, Branquinha, Marta H, Santos, André LS
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6174640/
https://www.ncbi.nlm.nih.gov/pubmed/30304087
http://dx.doi.org/10.1590/0074-02760180311
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author Aor, Ana Carolina
Mello, Thaís P
Sangenito, Leandro S
Fonseca, Beatriz B
Rozental, Sonia
Lione, Viviane F
Veiga, Venício F
Branquinha, Marta H
Santos, André LS
author_facet Aor, Ana Carolina
Mello, Thaís P
Sangenito, Leandro S
Fonseca, Beatriz B
Rozental, Sonia
Lione, Viviane F
Veiga, Venício F
Branquinha, Marta H
Santos, André LS
author_sort Aor, Ana Carolina
collection PubMed
description BACKGROUND: Scedosporium apiospermum is a ubiquitous, emerging and multidrug-resistant fungal pathogen with still rather unknown virulence mechanisms. OBJECTIVES/METHODS: The cellular basis of the in vitro interaction between fungi and host cells/tissues is the determinant factor for the development of a successful in vivo infection. Herein, we evaluated the interaction of S. apiospermum conidia with lung epithelial (A549), lung fibroblast (MRC-5) and RAW 264.7 macrophages by light and scanning/transmission electron microscopy. FINDINGS: After 4 h of fungi-host cell contact, the percentage of infected mammalian cells and the number of fungi per infected cell was measured by light microscopy, and the following association indexes were calculated for A549, MRC-5 and macrophage cells: 73.2 ± 25.9, 69.7 ± 22.5 and 59.7 ± 11.1, respectively. Both conidia and germinated conidia were regularly observed interacting with the evaluated cells, with a higher prevalence of non-germinated conidia. Interestingly, nests of germinated conidia were evidenced at the surface of lung cells by scanning electron microscopy. Some germination projections and hyphae were seen penetrating/evading the mammalian cells. Furthermore, internalised conidia were seen within vacuoles as visualised by transmission electron microscopy. MAIN CONCLUSIONS: The present study contributes to a better understanding of S. apiospermum pathogenesis by demonstrating the first steps of the infection process of this opportunistic fungus.
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spelling pubmed-61746402018-10-10 Ultrastructural viewpoints on the interaction events of Scedosporium apiospermum conidia with lung and macrophage cells Aor, Ana Carolina Mello, Thaís P Sangenito, Leandro S Fonseca, Beatriz B Rozental, Sonia Lione, Viviane F Veiga, Venício F Branquinha, Marta H Santos, André LS Mem Inst Oswaldo Cruz Original Article BACKGROUND: Scedosporium apiospermum is a ubiquitous, emerging and multidrug-resistant fungal pathogen with still rather unknown virulence mechanisms. OBJECTIVES/METHODS: The cellular basis of the in vitro interaction between fungi and host cells/tissues is the determinant factor for the development of a successful in vivo infection. Herein, we evaluated the interaction of S. apiospermum conidia with lung epithelial (A549), lung fibroblast (MRC-5) and RAW 264.7 macrophages by light and scanning/transmission electron microscopy. FINDINGS: After 4 h of fungi-host cell contact, the percentage of infected mammalian cells and the number of fungi per infected cell was measured by light microscopy, and the following association indexes were calculated for A549, MRC-5 and macrophage cells: 73.2 ± 25.9, 69.7 ± 22.5 and 59.7 ± 11.1, respectively. Both conidia and germinated conidia were regularly observed interacting with the evaluated cells, with a higher prevalence of non-germinated conidia. Interestingly, nests of germinated conidia were evidenced at the surface of lung cells by scanning electron microscopy. Some germination projections and hyphae were seen penetrating/evading the mammalian cells. Furthermore, internalised conidia were seen within vacuoles as visualised by transmission electron microscopy. MAIN CONCLUSIONS: The present study contributes to a better understanding of S. apiospermum pathogenesis by demonstrating the first steps of the infection process of this opportunistic fungus. Instituto Oswaldo Cruz, Ministério da Saúde 2018-10-08 /pmc/articles/PMC6174640/ /pubmed/30304087 http://dx.doi.org/10.1590/0074-02760180311 Text en https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Original Article
Aor, Ana Carolina
Mello, Thaís P
Sangenito, Leandro S
Fonseca, Beatriz B
Rozental, Sonia
Lione, Viviane F
Veiga, Venício F
Branquinha, Marta H
Santos, André LS
Ultrastructural viewpoints on the interaction events of Scedosporium apiospermum conidia with lung and macrophage cells
title Ultrastructural viewpoints on the interaction events of Scedosporium apiospermum conidia with lung and macrophage cells
title_full Ultrastructural viewpoints on the interaction events of Scedosporium apiospermum conidia with lung and macrophage cells
title_fullStr Ultrastructural viewpoints on the interaction events of Scedosporium apiospermum conidia with lung and macrophage cells
title_full_unstemmed Ultrastructural viewpoints on the interaction events of Scedosporium apiospermum conidia with lung and macrophage cells
title_short Ultrastructural viewpoints on the interaction events of Scedosporium apiospermum conidia with lung and macrophage cells
title_sort ultrastructural viewpoints on the interaction events of scedosporium apiospermum conidia with lung and macrophage cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6174640/
https://www.ncbi.nlm.nih.gov/pubmed/30304087
http://dx.doi.org/10.1590/0074-02760180311
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