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UDP-glycosyltransferase family in Haemonchus contortus: Phylogenetic analysis, constitutive expression, sex-differences and resistance-related differences
UDP-glycosyltransferases (UGT), catalysing conjugation of UDP-activated sugar donors to small lipophilic chemicals, are widespread in living organisms from bacteria to fungi, plant, or animals. The progress of genome sequencing has enabled an assessment of the UGT multigene family in Haemonchus cont...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6174829/ https://www.ncbi.nlm.nih.gov/pubmed/30293057 http://dx.doi.org/10.1016/j.ijpddr.2018.09.005 |
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author | Matoušková, Petra Lecová, Lenka Laing, Roz Dimunová, Diana Vogel, Heiko Raisová Stuchlíková, Lucie Nguyen, Linh Thuy Kellerová, Pavlína Vokřál, Ivan Lamka, Jiří Szotáková, Barbora Várady, Marián Skálová, Lenka |
author_facet | Matoušková, Petra Lecová, Lenka Laing, Roz Dimunová, Diana Vogel, Heiko Raisová Stuchlíková, Lucie Nguyen, Linh Thuy Kellerová, Pavlína Vokřál, Ivan Lamka, Jiří Szotáková, Barbora Várady, Marián Skálová, Lenka |
author_sort | Matoušková, Petra |
collection | PubMed |
description | UDP-glycosyltransferases (UGT), catalysing conjugation of UDP-activated sugar donors to small lipophilic chemicals, are widespread in living organisms from bacteria to fungi, plant, or animals. The progress of genome sequencing has enabled an assessment of the UGT multigene family in Haemonchus contortus (family Trichostrongylidae, Nematoda), a hematophagous gastrointestinal parasite of small ruminants. Here we report 32 putative UGT genes divided into 15 UGT families. Phylogenetic analysis in comparison with UGTs from Caenorhabditis elegans, a free-living model nematode, revealed several single member homologues, a lack of the dramatic gene expansion seen in C. elegans, but also several families (UGT365, UGT366, UGT368) expanded in H. contortus only. The assessment of constitutive UGT mRNA expression in H. contortus adults identified significant differences between females and males. In addition, we compared the expression of selected UGTs in the drug-sensitive ISE strain to two benzimidazole-resistant strains, IRE and WR, with different genetic backgrounds. Constitutive expression of UGT368B2 was significantly higher in both resistant strains than in the sensitive strain. As resistant strains were able to deactivate benzimidazole anthelmintics via glycosylation more effectively then the sensitive strain, UGT368B2 enhanced constitutive expression might contribute to drug resistance in H. contortus. |
format | Online Article Text |
id | pubmed-6174829 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-61748292018-10-09 UDP-glycosyltransferase family in Haemonchus contortus: Phylogenetic analysis, constitutive expression, sex-differences and resistance-related differences Matoušková, Petra Lecová, Lenka Laing, Roz Dimunová, Diana Vogel, Heiko Raisová Stuchlíková, Lucie Nguyen, Linh Thuy Kellerová, Pavlína Vokřál, Ivan Lamka, Jiří Szotáková, Barbora Várady, Marián Skálová, Lenka Int J Parasitol Drugs Drug Resist Regular article UDP-glycosyltransferases (UGT), catalysing conjugation of UDP-activated sugar donors to small lipophilic chemicals, are widespread in living organisms from bacteria to fungi, plant, or animals. The progress of genome sequencing has enabled an assessment of the UGT multigene family in Haemonchus contortus (family Trichostrongylidae, Nematoda), a hematophagous gastrointestinal parasite of small ruminants. Here we report 32 putative UGT genes divided into 15 UGT families. Phylogenetic analysis in comparison with UGTs from Caenorhabditis elegans, a free-living model nematode, revealed several single member homologues, a lack of the dramatic gene expansion seen in C. elegans, but also several families (UGT365, UGT366, UGT368) expanded in H. contortus only. The assessment of constitutive UGT mRNA expression in H. contortus adults identified significant differences between females and males. In addition, we compared the expression of selected UGTs in the drug-sensitive ISE strain to two benzimidazole-resistant strains, IRE and WR, with different genetic backgrounds. Constitutive expression of UGT368B2 was significantly higher in both resistant strains than in the sensitive strain. As resistant strains were able to deactivate benzimidazole anthelmintics via glycosylation more effectively then the sensitive strain, UGT368B2 enhanced constitutive expression might contribute to drug resistance in H. contortus. Elsevier 2018-09-26 /pmc/articles/PMC6174829/ /pubmed/30293057 http://dx.doi.org/10.1016/j.ijpddr.2018.09.005 Text en © 2018 The Authors. Published by Elsevier Ltd on behalf of Australian Society for Parasitology. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Regular article Matoušková, Petra Lecová, Lenka Laing, Roz Dimunová, Diana Vogel, Heiko Raisová Stuchlíková, Lucie Nguyen, Linh Thuy Kellerová, Pavlína Vokřál, Ivan Lamka, Jiří Szotáková, Barbora Várady, Marián Skálová, Lenka UDP-glycosyltransferase family in Haemonchus contortus: Phylogenetic analysis, constitutive expression, sex-differences and resistance-related differences |
title | UDP-glycosyltransferase family in Haemonchus contortus: Phylogenetic analysis, constitutive expression, sex-differences and resistance-related differences |
title_full | UDP-glycosyltransferase family in Haemonchus contortus: Phylogenetic analysis, constitutive expression, sex-differences and resistance-related differences |
title_fullStr | UDP-glycosyltransferase family in Haemonchus contortus: Phylogenetic analysis, constitutive expression, sex-differences and resistance-related differences |
title_full_unstemmed | UDP-glycosyltransferase family in Haemonchus contortus: Phylogenetic analysis, constitutive expression, sex-differences and resistance-related differences |
title_short | UDP-glycosyltransferase family in Haemonchus contortus: Phylogenetic analysis, constitutive expression, sex-differences and resistance-related differences |
title_sort | udp-glycosyltransferase family in haemonchus contortus: phylogenetic analysis, constitutive expression, sex-differences and resistance-related differences |
topic | Regular article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6174829/ https://www.ncbi.nlm.nih.gov/pubmed/30293057 http://dx.doi.org/10.1016/j.ijpddr.2018.09.005 |
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