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Caveolin-1 and -2 regulate cell motility in castration-resistant prostate cancer

BACKGROUND: Caveolin (Cav)-1 and Cav-2 are cell membrane proteins, which are structural proteins of caveolae and are reported to be positive regulators of cell survival and metastasis in prostate cancer (PC). In a previous study, we reported that elevated levels of Cav-1 and Cav-2 were significantly...

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Autores principales: Kamibeppu, Toyoharu, Yamasaki, Koji, Nakahara, Kozue, Nagai, Takahiro, Terada, Naoki, Tsukino, Hiromasa, Mukai, Shoichiro, Kamoto, Toshiyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6174915/
https://www.ncbi.nlm.nih.gov/pubmed/30324095
http://dx.doi.org/10.2147/RRU.S173377
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author Kamibeppu, Toyoharu
Yamasaki, Koji
Nakahara, Kozue
Nagai, Takahiro
Terada, Naoki
Tsukino, Hiromasa
Mukai, Shoichiro
Kamoto, Toshiyuki
author_facet Kamibeppu, Toyoharu
Yamasaki, Koji
Nakahara, Kozue
Nagai, Takahiro
Terada, Naoki
Tsukino, Hiromasa
Mukai, Shoichiro
Kamoto, Toshiyuki
author_sort Kamibeppu, Toyoharu
collection PubMed
description BACKGROUND: Caveolin (Cav)-1 and Cav-2 are cell membrane proteins, which are structural proteins of caveolae and are reported to be positive regulators of cell survival and metastasis in prostate cancer (PC). In a previous study, we reported that elevated levels of Cav-1 and Cav-2 were significantly associated with PC progression. However, their functions in PC have not yet been clarified. In this study, we examined the function of Cav-1 and Cav-2 in PC cell invasiveness and motility. MATERIALS AND METHODS: We introduced Cav-1- and Cav-2-specific small interfering into PC3 cells to knock-down (KD) both molecules. We also performed cell proliferation assay, wound healing assay, migration assay, and invasion assay using PC3 cells and compared the results between Cav-1-KD, Cav-2-KD, and negative control PC3 cells. In addition, we performed real-time quantitative PCR (RT-qPCR) and RT2 Profiler PCR Array analysis to identify factors influencing migration. RESULTS: We observed no significant difference in the proliferative and invasive activities of Cav-1-KD and Cav-2-KD PC3 cells; however, the cell motility was significantly decreased compared with negative control PC3 cells. RT-qPCR revealed that the expression of vimentin and N-cadherin was downregulated in Cav-1-KD PC3 cells. In addition, PCR array revealed a decreased expression of MGAT5, MMP13, and MYCL in Cav-1-KD PC3 and ETV4, FGFR4, and SRC in Cav-2-KD PC3. CONCLUSION: Cav-1 and Cav-2 may positively contribute to the upregulation of castration-resistant PC cell migration. Cav-induced regulation of several molecules including vimentin, N-cadherin, MGAT5, MMP13, MYCL, ETV4, FGFR4, and SRC may have an important role in PC3 cell motility. However, further examination will be required.
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spelling pubmed-61749152018-10-15 Caveolin-1 and -2 regulate cell motility in castration-resistant prostate cancer Kamibeppu, Toyoharu Yamasaki, Koji Nakahara, Kozue Nagai, Takahiro Terada, Naoki Tsukino, Hiromasa Mukai, Shoichiro Kamoto, Toshiyuki Res Rep Urol Original Research BACKGROUND: Caveolin (Cav)-1 and Cav-2 are cell membrane proteins, which are structural proteins of caveolae and are reported to be positive regulators of cell survival and metastasis in prostate cancer (PC). In a previous study, we reported that elevated levels of Cav-1 and Cav-2 were significantly associated with PC progression. However, their functions in PC have not yet been clarified. In this study, we examined the function of Cav-1 and Cav-2 in PC cell invasiveness and motility. MATERIALS AND METHODS: We introduced Cav-1- and Cav-2-specific small interfering into PC3 cells to knock-down (KD) both molecules. We also performed cell proliferation assay, wound healing assay, migration assay, and invasion assay using PC3 cells and compared the results between Cav-1-KD, Cav-2-KD, and negative control PC3 cells. In addition, we performed real-time quantitative PCR (RT-qPCR) and RT2 Profiler PCR Array analysis to identify factors influencing migration. RESULTS: We observed no significant difference in the proliferative and invasive activities of Cav-1-KD and Cav-2-KD PC3 cells; however, the cell motility was significantly decreased compared with negative control PC3 cells. RT-qPCR revealed that the expression of vimentin and N-cadherin was downregulated in Cav-1-KD PC3 cells. In addition, PCR array revealed a decreased expression of MGAT5, MMP13, and MYCL in Cav-1-KD PC3 and ETV4, FGFR4, and SRC in Cav-2-KD PC3. CONCLUSION: Cav-1 and Cav-2 may positively contribute to the upregulation of castration-resistant PC cell migration. Cav-induced regulation of several molecules including vimentin, N-cadherin, MGAT5, MMP13, MYCL, ETV4, FGFR4, and SRC may have an important role in PC3 cell motility. However, further examination will be required. Dove Medical Press 2018-10-03 /pmc/articles/PMC6174915/ /pubmed/30324095 http://dx.doi.org/10.2147/RRU.S173377 Text en © 2018 Kamibeppu et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Kamibeppu, Toyoharu
Yamasaki, Koji
Nakahara, Kozue
Nagai, Takahiro
Terada, Naoki
Tsukino, Hiromasa
Mukai, Shoichiro
Kamoto, Toshiyuki
Caveolin-1 and -2 regulate cell motility in castration-resistant prostate cancer
title Caveolin-1 and -2 regulate cell motility in castration-resistant prostate cancer
title_full Caveolin-1 and -2 regulate cell motility in castration-resistant prostate cancer
title_fullStr Caveolin-1 and -2 regulate cell motility in castration-resistant prostate cancer
title_full_unstemmed Caveolin-1 and -2 regulate cell motility in castration-resistant prostate cancer
title_short Caveolin-1 and -2 regulate cell motility in castration-resistant prostate cancer
title_sort caveolin-1 and -2 regulate cell motility in castration-resistant prostate cancer
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6174915/
https://www.ncbi.nlm.nih.gov/pubmed/30324095
http://dx.doi.org/10.2147/RRU.S173377
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