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Renal expression of trefoil factor 3 mRNA in association with tubulointerstitial fibrosis in IgA nephropathy

AIM: Trefoil factor 3 (TFF3) is a small peptide that is involved in mucosal protection. TFF3 is widely expressed in multiple tissues including kidney tissue. Previous studies have reported that the levels of urinary TFF3 are significantly increased in patients with chronic kidney disease. The aim of...

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Autores principales: Tanaka, Keiko, Sugiyama, Hitoshi, Yamanari, Toshio, Mise, Koki, Morinaga, Hiroshi, Kitagawa, Masashi, Onishi, Akifumi, Ogawa‐Akiyama, Ayu, Tanabe, Katsuyuki, Eguchi, Jun, Ohmoto, Yasukazu, Shikata, Kenichi, Wada, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons Australia, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6174951/
https://www.ncbi.nlm.nih.gov/pubmed/29987860
http://dx.doi.org/10.1111/nep.13444
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author Tanaka, Keiko
Sugiyama, Hitoshi
Yamanari, Toshio
Mise, Koki
Morinaga, Hiroshi
Kitagawa, Masashi
Onishi, Akifumi
Ogawa‐Akiyama, Ayu
Tanabe, Katsuyuki
Eguchi, Jun
Ohmoto, Yasukazu
Shikata, Kenichi
Wada, Jun
author_facet Tanaka, Keiko
Sugiyama, Hitoshi
Yamanari, Toshio
Mise, Koki
Morinaga, Hiroshi
Kitagawa, Masashi
Onishi, Akifumi
Ogawa‐Akiyama, Ayu
Tanabe, Katsuyuki
Eguchi, Jun
Ohmoto, Yasukazu
Shikata, Kenichi
Wada, Jun
author_sort Tanaka, Keiko
collection PubMed
description AIM: Trefoil factor 3 (TFF3) is a small peptide that is involved in mucosal protection. TFF3 is widely expressed in multiple tissues including kidney tissue. Previous studies have reported that the levels of urinary TFF3 are significantly increased in patients with chronic kidney disease. The aim of this study is to detect the TFF3 mRNA in kidney and elucidate the relationship between renal TFF3 mRNA and tubulointerstitial fibrosis in IgA nephropathy (IgAN). METHODS: We investigated the renal mRNA expression of TFF3 by real‐time PCR analysis in biopsy specimens from patients with IgAN, other glomerulonephritis (OGN) and minor glomerular abnormalities (MGA). We also determined the renal localization of TFF3 and the levels of urinary TFF3 by immunostaining and ELISA, respectively. RESULTS: The renal TFF3 mRNA expression was significantly associated with the urinary TFF3 secretion and the tubulointerstitial fibrosis score in the IgAN group alone. Immunostaining of the renal specimen of IgAN patients revealed that TFF3 is located in the renal tubular epithelial cells. The locations were almost the same as those that showed uromodulin positivity; specifically, the thick ascending limb (TAL) of the loop of Henle and the early portion of the distal tubule. The urinary TFF3 levels were positively correlated with the levels of urinary biomarkers of tubulointerstitial injury in such patients. CONCLUSION: Renal TFF3 mRNA is associated with renal tubulointerstitial fibrosis in IgAN patients. The TFF3 located in the renal tubular epithelial cells may play a role in the progression of tubulointerstitial fibrosis in IgAN patients.
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spelling pubmed-61749512018-10-15 Renal expression of trefoil factor 3 mRNA in association with tubulointerstitial fibrosis in IgA nephropathy Tanaka, Keiko Sugiyama, Hitoshi Yamanari, Toshio Mise, Koki Morinaga, Hiroshi Kitagawa, Masashi Onishi, Akifumi Ogawa‐Akiyama, Ayu Tanabe, Katsuyuki Eguchi, Jun Ohmoto, Yasukazu Shikata, Kenichi Wada, Jun Nephrology (Carlton) Original Articles AIM: Trefoil factor 3 (TFF3) is a small peptide that is involved in mucosal protection. TFF3 is widely expressed in multiple tissues including kidney tissue. Previous studies have reported that the levels of urinary TFF3 are significantly increased in patients with chronic kidney disease. The aim of this study is to detect the TFF3 mRNA in kidney and elucidate the relationship between renal TFF3 mRNA and tubulointerstitial fibrosis in IgA nephropathy (IgAN). METHODS: We investigated the renal mRNA expression of TFF3 by real‐time PCR analysis in biopsy specimens from patients with IgAN, other glomerulonephritis (OGN) and minor glomerular abnormalities (MGA). We also determined the renal localization of TFF3 and the levels of urinary TFF3 by immunostaining and ELISA, respectively. RESULTS: The renal TFF3 mRNA expression was significantly associated with the urinary TFF3 secretion and the tubulointerstitial fibrosis score in the IgAN group alone. Immunostaining of the renal specimen of IgAN patients revealed that TFF3 is located in the renal tubular epithelial cells. The locations were almost the same as those that showed uromodulin positivity; specifically, the thick ascending limb (TAL) of the loop of Henle and the early portion of the distal tubule. The urinary TFF3 levels were positively correlated with the levels of urinary biomarkers of tubulointerstitial injury in such patients. CONCLUSION: Renal TFF3 mRNA is associated with renal tubulointerstitial fibrosis in IgAN patients. The TFF3 located in the renal tubular epithelial cells may play a role in the progression of tubulointerstitial fibrosis in IgAN patients. John Wiley & Sons Australia, Ltd 2018-07-26 2018-09 /pmc/articles/PMC6174951/ /pubmed/29987860 http://dx.doi.org/10.1111/nep.13444 Text en © 2018 The Authors Nephrology published by John Wiley & Sons Australia, Ltd on behalf of Asian Pacific Society of Nephrology This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Tanaka, Keiko
Sugiyama, Hitoshi
Yamanari, Toshio
Mise, Koki
Morinaga, Hiroshi
Kitagawa, Masashi
Onishi, Akifumi
Ogawa‐Akiyama, Ayu
Tanabe, Katsuyuki
Eguchi, Jun
Ohmoto, Yasukazu
Shikata, Kenichi
Wada, Jun
Renal expression of trefoil factor 3 mRNA in association with tubulointerstitial fibrosis in IgA nephropathy
title Renal expression of trefoil factor 3 mRNA in association with tubulointerstitial fibrosis in IgA nephropathy
title_full Renal expression of trefoil factor 3 mRNA in association with tubulointerstitial fibrosis in IgA nephropathy
title_fullStr Renal expression of trefoil factor 3 mRNA in association with tubulointerstitial fibrosis in IgA nephropathy
title_full_unstemmed Renal expression of trefoil factor 3 mRNA in association with tubulointerstitial fibrosis in IgA nephropathy
title_short Renal expression of trefoil factor 3 mRNA in association with tubulointerstitial fibrosis in IgA nephropathy
title_sort renal expression of trefoil factor 3 mrna in association with tubulointerstitial fibrosis in iga nephropathy
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6174951/
https://www.ncbi.nlm.nih.gov/pubmed/29987860
http://dx.doi.org/10.1111/nep.13444
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