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Dynamic changes in intrathymic ILC populations during murine neonatal development
Members of the innate lymphoid cell (ILC) family have been implicated in the development of thymic microenvironments and the recovery of this architecture after damage. However, a detailed characterization of this family in the thymus is lacking. To better understand the thymic ILC compartment, we h...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6174991/ https://www.ncbi.nlm.nih.gov/pubmed/29851080 http://dx.doi.org/10.1002/eji.201847511 |
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author | Jones, Rhys Cosway, Emilie J. Willis, Claire White, Andrea J. Jenkinson, William E. Fehling, Hans J. Anderson, Graham Withers, David R. |
author_facet | Jones, Rhys Cosway, Emilie J. Willis, Claire White, Andrea J. Jenkinson, William E. Fehling, Hans J. Anderson, Graham Withers, David R. |
author_sort | Jones, Rhys |
collection | PubMed |
description | Members of the innate lymphoid cell (ILC) family have been implicated in the development of thymic microenvironments and the recovery of this architecture after damage. However, a detailed characterization of this family in the thymus is lacking. To better understand the thymic ILC compartment, we have utilized multiple in vivo models including the fate mapping of inhibitor of DNA binding‐2 (Id2) expression and the use of Id2 reporter mice. Our data demonstrate that ILCs are more prominent immediately after birth, but were rapidly diluted as the T‐cell development program increased. As observed in the embryonic thymus, CCR6(+)NKp46(−) lymphoid tissue inducer (LTi) cells were the main ILC3 population present, but numbers of these cells swiftly declined in the neonate and ILC3 were barely detectable in adult thymus. This loss of ILC3 means ILC2 are the dominant ILC population in the thymus. Thymic ILC2 were able to produce IL‐5 and IL‐13, were located within the medulla, and did not result from ILC3 plasticity. Furthermore, in WT mice, thymic ILC2 express little RANKL (receptor activator of nuclear factor kappa‐B ligand) arguing that functionally, these cells provide different signals to LTi cells in the thymus. Collectively, these data reveal a dynamic switch in the ILC populations of the thymus during neonatal development. |
format | Online Article Text |
id | pubmed-6174991 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-61749912018-10-15 Dynamic changes in intrathymic ILC populations during murine neonatal development Jones, Rhys Cosway, Emilie J. Willis, Claire White, Andrea J. Jenkinson, William E. Fehling, Hans J. Anderson, Graham Withers, David R. Eur J Immunol Leukocyte and lymphoid organ ontogeny Members of the innate lymphoid cell (ILC) family have been implicated in the development of thymic microenvironments and the recovery of this architecture after damage. However, a detailed characterization of this family in the thymus is lacking. To better understand the thymic ILC compartment, we have utilized multiple in vivo models including the fate mapping of inhibitor of DNA binding‐2 (Id2) expression and the use of Id2 reporter mice. Our data demonstrate that ILCs are more prominent immediately after birth, but were rapidly diluted as the T‐cell development program increased. As observed in the embryonic thymus, CCR6(+)NKp46(−) lymphoid tissue inducer (LTi) cells were the main ILC3 population present, but numbers of these cells swiftly declined in the neonate and ILC3 were barely detectable in adult thymus. This loss of ILC3 means ILC2 are the dominant ILC population in the thymus. Thymic ILC2 were able to produce IL‐5 and IL‐13, were located within the medulla, and did not result from ILC3 plasticity. Furthermore, in WT mice, thymic ILC2 express little RANKL (receptor activator of nuclear factor kappa‐B ligand) arguing that functionally, these cells provide different signals to LTi cells in the thymus. Collectively, these data reveal a dynamic switch in the ILC populations of the thymus during neonatal development. John Wiley and Sons Inc. 2018-07-02 2018-09 /pmc/articles/PMC6174991/ /pubmed/29851080 http://dx.doi.org/10.1002/eji.201847511 Text en © 2018 The Authors. European Journal of Immunology published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Leukocyte and lymphoid organ ontogeny Jones, Rhys Cosway, Emilie J. Willis, Claire White, Andrea J. Jenkinson, William E. Fehling, Hans J. Anderson, Graham Withers, David R. Dynamic changes in intrathymic ILC populations during murine neonatal development |
title | Dynamic changes in intrathymic ILC populations during murine neonatal development |
title_full | Dynamic changes in intrathymic ILC populations during murine neonatal development |
title_fullStr | Dynamic changes in intrathymic ILC populations during murine neonatal development |
title_full_unstemmed | Dynamic changes in intrathymic ILC populations during murine neonatal development |
title_short | Dynamic changes in intrathymic ILC populations during murine neonatal development |
title_sort | dynamic changes in intrathymic ilc populations during murine neonatal development |
topic | Leukocyte and lymphoid organ ontogeny |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6174991/ https://www.ncbi.nlm.nih.gov/pubmed/29851080 http://dx.doi.org/10.1002/eji.201847511 |
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