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Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide

Lonicera japonica is a typical Chinese herbal medicine. We previously reported a method to isolate polysaccharides from Lonicera japonica (LJP). In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide c...

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Autores principales: Zhou, Xiaonan, Dong, Qun, Kan, Xianzhao, Peng, Lihong, Xu, Xingyu, Fang, Yun, Yang, Jialiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175272/
https://www.ncbi.nlm.nih.gov/pubmed/30296293
http://dx.doi.org/10.1371/journal.pone.0204152
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author Zhou, Xiaonan
Dong, Qun
Kan, Xianzhao
Peng, Lihong
Xu, Xingyu
Fang, Yun
Yang, Jialiang
author_facet Zhou, Xiaonan
Dong, Qun
Kan, Xianzhao
Peng, Lihong
Xu, Xingyu
Fang, Yun
Yang, Jialiang
author_sort Zhou, Xiaonan
collection PubMed
description Lonicera japonica is a typical Chinese herbal medicine. We previously reported a method to isolate polysaccharides from Lonicera japonica (LJP). In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method. We then investigated the immunomodulatory function of LJP in cyclophosphamide (CTX)-induced immunosuppressed mouse models. The results showed that LJP had the characteristic absorption of typical polysaccharides consisting of 6 types of monosaccharides. In addition, LJP can increase significantly the organ index, splenic lymphocyte proliferation, macrophage phagocytosis, and natural killer (NK) cell activity in CTX-treated mice. LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice. Finally, the results on measuring the T-lymphocytes subsets of spleen also confirmed LJP-induced immunomodulatory activity in immunosuppressed mice from another perspective. Therefore, LJP could be used as a potential immunomodulatory agent.
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spelling pubmed-61752722018-10-19 Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide Zhou, Xiaonan Dong, Qun Kan, Xianzhao Peng, Lihong Xu, Xingyu Fang, Yun Yang, Jialiang PLoS One Research Article Lonicera japonica is a typical Chinese herbal medicine. We previously reported a method to isolate polysaccharides from Lonicera japonica (LJP). In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method. We then investigated the immunomodulatory function of LJP in cyclophosphamide (CTX)-induced immunosuppressed mouse models. The results showed that LJP had the characteristic absorption of typical polysaccharides consisting of 6 types of monosaccharides. In addition, LJP can increase significantly the organ index, splenic lymphocyte proliferation, macrophage phagocytosis, and natural killer (NK) cell activity in CTX-treated mice. LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice. Finally, the results on measuring the T-lymphocytes subsets of spleen also confirmed LJP-induced immunomodulatory activity in immunosuppressed mice from another perspective. Therefore, LJP could be used as a potential immunomodulatory agent. Public Library of Science 2018-10-08 /pmc/articles/PMC6175272/ /pubmed/30296293 http://dx.doi.org/10.1371/journal.pone.0204152 Text en © 2018 Zhou et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhou, Xiaonan
Dong, Qun
Kan, Xianzhao
Peng, Lihong
Xu, Xingyu
Fang, Yun
Yang, Jialiang
Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide
title Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide
title_full Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide
title_fullStr Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide
title_full_unstemmed Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide
title_short Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice induced by cyclophosphamide
title_sort immunomodulatory activity of a novel polysaccharide from lonicera japonica in immunosuppressed mice induced by cyclophosphamide
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175272/
https://www.ncbi.nlm.nih.gov/pubmed/30296293
http://dx.doi.org/10.1371/journal.pone.0204152
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