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An efficient method to isolate Kupffer cells eliminating endothelial cell contamination and selective bias

Multicolor flow cytometry and cell sorting are powerful immunologic tools for the study of hepatic mϕ, yet there is no consensus on the optimal method to prepare liver homogenates for these analyses. Using a combination of mϕ and endothelial cell reporter mice, flow cytometry, and confocal imaging,...

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Detalles Bibliográficos
Autores principales:	Lynch, Ruairi W., Hawley, Catherine A., Pellicoro, Antonella, Bain, Calum C., Iredale, John P., Jenkins, Stephen J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2018
Materias:	Cell Development, Differentiation, & Trafficking
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175317/ https://www.ncbi.nlm.nih.gov/pubmed/29607532 http://dx.doi.org/10.1002/JLB.1TA0517-169R

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