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Genome‐wide association study reveals novel players in defense hormone crosstalk in Arabidopsis
Jasmonic acid (JA) regulates plant defenses against necrotrophic pathogens and insect herbivores. Salicylic acid (SA) and abscisic acid (ABA) can antagonize JA‐regulated defenses, thereby modulating pathogen or insect resistance. We performed a genome‐wide association (GWA) study on natural genetic...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175328/ https://www.ncbi.nlm.nih.gov/pubmed/29852537 http://dx.doi.org/10.1111/pce.13357 |
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author | Proietti, Silvia Caarls, Lotte Coolen, Silvia Van Pelt, Johan A. Van Wees, Saskia C.M. Pieterse, Corné M.J. |
author_facet | Proietti, Silvia Caarls, Lotte Coolen, Silvia Van Pelt, Johan A. Van Wees, Saskia C.M. Pieterse, Corné M.J. |
author_sort | Proietti, Silvia |
collection | PubMed |
description | Jasmonic acid (JA) regulates plant defenses against necrotrophic pathogens and insect herbivores. Salicylic acid (SA) and abscisic acid (ABA) can antagonize JA‐regulated defenses, thereby modulating pathogen or insect resistance. We performed a genome‐wide association (GWA) study on natural genetic variation in Arabidopsis thaliana for the effect of SA and ABA on the JA pathway. We treated 349 Arabidopsis accessions with methyl JA (MeJA), or a combination of MeJA and either SA or ABA, after which expression of the JA‐responsive marker gene PLANT DEFENSIN1.2 (PDF1.2) was quantified as a readout for GWA analysis. Both hormones antagonized MeJA‐induced PDF1.2 in the majority of the accessions but with a large variation in magnitude. GWA mapping of the SA‐ and ABA‐affected PDF1.2 expression data revealed loci associated with crosstalk. GLYI4 (encoding a glyoxalase) and ARR11 (encoding an Arabidopsis response regulator involved in cytokinin signalling) were confirmed by T‐DNA insertion mutant analysis to affect SA–JA crosstalk and resistance against the necrotroph Botrytis cinerea. In addition, At1g16310 (encoding a cation efflux family protein) was confirmed to affect ABA–JA crosstalk and susceptibility to Mamestra brassicae herbivory. Collectively, this GWA study identified novel players in JA hormone crosstalk with potential roles in the regulation of pathogen or insect resistance. |
format | Online Article Text |
id | pubmed-6175328 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-61753282018-10-15 Genome‐wide association study reveals novel players in defense hormone crosstalk in Arabidopsis Proietti, Silvia Caarls, Lotte Coolen, Silvia Van Pelt, Johan A. Van Wees, Saskia C.M. Pieterse, Corné M.J. Plant Cell Environ Original Articles Jasmonic acid (JA) regulates plant defenses against necrotrophic pathogens and insect herbivores. Salicylic acid (SA) and abscisic acid (ABA) can antagonize JA‐regulated defenses, thereby modulating pathogen or insect resistance. We performed a genome‐wide association (GWA) study on natural genetic variation in Arabidopsis thaliana for the effect of SA and ABA on the JA pathway. We treated 349 Arabidopsis accessions with methyl JA (MeJA), or a combination of MeJA and either SA or ABA, after which expression of the JA‐responsive marker gene PLANT DEFENSIN1.2 (PDF1.2) was quantified as a readout for GWA analysis. Both hormones antagonized MeJA‐induced PDF1.2 in the majority of the accessions but with a large variation in magnitude. GWA mapping of the SA‐ and ABA‐affected PDF1.2 expression data revealed loci associated with crosstalk. GLYI4 (encoding a glyoxalase) and ARR11 (encoding an Arabidopsis response regulator involved in cytokinin signalling) were confirmed by T‐DNA insertion mutant analysis to affect SA–JA crosstalk and resistance against the necrotroph Botrytis cinerea. In addition, At1g16310 (encoding a cation efflux family protein) was confirmed to affect ABA–JA crosstalk and susceptibility to Mamestra brassicae herbivory. Collectively, this GWA study identified novel players in JA hormone crosstalk with potential roles in the regulation of pathogen or insect resistance. John Wiley and Sons Inc. 2018-07-03 2018-10 /pmc/articles/PMC6175328/ /pubmed/29852537 http://dx.doi.org/10.1111/pce.13357 Text en © 2018 The Authors. Plant, Cell & Environment Published by John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Proietti, Silvia Caarls, Lotte Coolen, Silvia Van Pelt, Johan A. Van Wees, Saskia C.M. Pieterse, Corné M.J. Genome‐wide association study reveals novel players in defense hormone crosstalk in Arabidopsis |
title | Genome‐wide association study reveals novel players in defense hormone crosstalk in Arabidopsis
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title_full | Genome‐wide association study reveals novel players in defense hormone crosstalk in Arabidopsis
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title_fullStr | Genome‐wide association study reveals novel players in defense hormone crosstalk in Arabidopsis
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title_full_unstemmed | Genome‐wide association study reveals novel players in defense hormone crosstalk in Arabidopsis
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title_short | Genome‐wide association study reveals novel players in defense hormone crosstalk in Arabidopsis
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title_sort | genome‐wide association study reveals novel players in defense hormone crosstalk in arabidopsis |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175328/ https://www.ncbi.nlm.nih.gov/pubmed/29852537 http://dx.doi.org/10.1111/pce.13357 |
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