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Using a phiC31 “Disintegrase” to make new attP sites in the Drosophila genome at locations showing chromosomal position effects
An engineered phiC31 “Disintegrase” able to make an attP site in Drosophila out of an attR-attL pair is described. This was used to generate attP sites at genomic locations where a mini-white (mini-w) transgene was subject to chromosomal position effects (CPE). The first step was random genomic inte...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175522/ https://www.ncbi.nlm.nih.gov/pubmed/30296303 http://dx.doi.org/10.1371/journal.pone.0205538 |