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Changes in the genetic requirements for microbial interactions with increasing community complexity
Microbial community structure and function rely on complex interactions whose underlying molecular mechanisms are poorly understood. To investigate these interactions in a simple microbiome, we introduced E. coli into an experimental community based on a cheese rind and identified the differences in...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175579/ https://www.ncbi.nlm.nih.gov/pubmed/30211673 http://dx.doi.org/10.7554/eLife.37072 |
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author | Morin, Manon Pierce, Emily C Dutton, Rachel J |
author_facet | Morin, Manon Pierce, Emily C Dutton, Rachel J |
author_sort | Morin, Manon |
collection | PubMed |
description | Microbial community structure and function rely on complex interactions whose underlying molecular mechanisms are poorly understood. To investigate these interactions in a simple microbiome, we introduced E. coli into an experimental community based on a cheese rind and identified the differences in E. coli’s genetic requirements for growth in interactive and non-interactive contexts using Random Barcode Transposon Sequencing (RB-TnSeq) and RNASeq. Genetic requirements varied among pairwise growth conditions and between pairwise and community conditions. Our analysis points to mechanisms by which growth conditions change as a result of increasing community complexity and suggests that growth within a community relies on a combination of pairwise and higher-order interactions. Our work provides a framework for using the model organism E. coli as a readout to investigate microbial interactions regardless of the genetic tractability of members of the studied ecosystem. |
format | Online Article Text |
id | pubmed-6175579 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-61755792018-10-15 Changes in the genetic requirements for microbial interactions with increasing community complexity Morin, Manon Pierce, Emily C Dutton, Rachel J eLife Microbiology and Infectious Disease Microbial community structure and function rely on complex interactions whose underlying molecular mechanisms are poorly understood. To investigate these interactions in a simple microbiome, we introduced E. coli into an experimental community based on a cheese rind and identified the differences in E. coli’s genetic requirements for growth in interactive and non-interactive contexts using Random Barcode Transposon Sequencing (RB-TnSeq) and RNASeq. Genetic requirements varied among pairwise growth conditions and between pairwise and community conditions. Our analysis points to mechanisms by which growth conditions change as a result of increasing community complexity and suggests that growth within a community relies on a combination of pairwise and higher-order interactions. Our work provides a framework for using the model organism E. coli as a readout to investigate microbial interactions regardless of the genetic tractability of members of the studied ecosystem. eLife Sciences Publications, Ltd 2018-09-13 /pmc/articles/PMC6175579/ /pubmed/30211673 http://dx.doi.org/10.7554/eLife.37072 Text en © 2018, Morin et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Microbiology and Infectious Disease Morin, Manon Pierce, Emily C Dutton, Rachel J Changes in the genetic requirements for microbial interactions with increasing community complexity |
title | Changes in the genetic requirements for microbial interactions with increasing community complexity |
title_full | Changes in the genetic requirements for microbial interactions with increasing community complexity |
title_fullStr | Changes in the genetic requirements for microbial interactions with increasing community complexity |
title_full_unstemmed | Changes in the genetic requirements for microbial interactions with increasing community complexity |
title_short | Changes in the genetic requirements for microbial interactions with increasing community complexity |
title_sort | changes in the genetic requirements for microbial interactions with increasing community complexity |
topic | Microbiology and Infectious Disease |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175579/ https://www.ncbi.nlm.nih.gov/pubmed/30211673 http://dx.doi.org/10.7554/eLife.37072 |
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