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Extraction and liposome reconstitution of membrane proteins with their native lipids without the use of detergents
Functional studies of membrane-bound channels, transporters or signal transducers require that the protein of interest resides in a membrane that separates two compartments. One approach that is commonly used to prepare these systems is to reconstitute the protein in liposomes. An intermediate step...
Autores principales: | Smirnova, Irina A., Ädelroth, Pia, Brzezinski, Peter |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175888/ https://www.ncbi.nlm.nih.gov/pubmed/30297885 http://dx.doi.org/10.1038/s41598-018-33208-1 |
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