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The Duality of the MAPK Signaling Pathway in the Control of Metabolic Processes and Cellulase Production in Trichoderma reesei
In this study, through global transcriptional analysis by RNA-Sequencing, we identified the main changes in gene expression that occurred in two functional mutants of the MAPK genes tmk1 and tmk2 in Trichoderma reesei during sugarcane bagasse degradation. We found that the proteins encoded by these...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175961/ https://www.ncbi.nlm.nih.gov/pubmed/30297963 http://dx.doi.org/10.1038/s41598-018-33383-1 |
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author | de Paula, Renato Graciano Antoniêto, Amanda Cristina Campos Carraro, Cláudia Batista Lopes, Douglas Christian Borges Persinoti, Gabriela Felix Peres, Nalu Teixeira Aguiar Martinez-Rossi, Nilce Maria Silva-Rocha, Rafael Silva, Roberto Nascimento |
author_facet | de Paula, Renato Graciano Antoniêto, Amanda Cristina Campos Carraro, Cláudia Batista Lopes, Douglas Christian Borges Persinoti, Gabriela Felix Peres, Nalu Teixeira Aguiar Martinez-Rossi, Nilce Maria Silva-Rocha, Rafael Silva, Roberto Nascimento |
author_sort | de Paula, Renato Graciano |
collection | PubMed |
description | In this study, through global transcriptional analysis by RNA-Sequencing, we identified the main changes in gene expression that occurred in two functional mutants of the MAPK genes tmk1 and tmk2 in Trichoderma reesei during sugarcane bagasse degradation. We found that the proteins encoded by these genes regulated independent processes, sometimes in a cross-talk manner, to modulate gene expression in T. reesei. In the Δtmk2 strain, growth in sugarcane bagasse modulated the expression of genes involved in carbohydrate metabolism, cell growth and development, and G-protein-coupled receptor-mediated cell signaling. On the other hand, deletion of tmk1 led to decreased expression of the major genes for cellulases and xylanases. Furthermore, TMK1 found to be involved in the regulation of the expression of major facilitator superfamily transporters. Our results revealed that the MAPK signaling pathway in T. reesei regulates many important processes that allow the fungus to recognize, transport, and metabolize different carbon sources during plant cell wall degradation. |
format | Online Article Text |
id | pubmed-6175961 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-61759612018-10-12 The Duality of the MAPK Signaling Pathway in the Control of Metabolic Processes and Cellulase Production in Trichoderma reesei de Paula, Renato Graciano Antoniêto, Amanda Cristina Campos Carraro, Cláudia Batista Lopes, Douglas Christian Borges Persinoti, Gabriela Felix Peres, Nalu Teixeira Aguiar Martinez-Rossi, Nilce Maria Silva-Rocha, Rafael Silva, Roberto Nascimento Sci Rep Article In this study, through global transcriptional analysis by RNA-Sequencing, we identified the main changes in gene expression that occurred in two functional mutants of the MAPK genes tmk1 and tmk2 in Trichoderma reesei during sugarcane bagasse degradation. We found that the proteins encoded by these genes regulated independent processes, sometimes in a cross-talk manner, to modulate gene expression in T. reesei. In the Δtmk2 strain, growth in sugarcane bagasse modulated the expression of genes involved in carbohydrate metabolism, cell growth and development, and G-protein-coupled receptor-mediated cell signaling. On the other hand, deletion of tmk1 led to decreased expression of the major genes for cellulases and xylanases. Furthermore, TMK1 found to be involved in the regulation of the expression of major facilitator superfamily transporters. Our results revealed that the MAPK signaling pathway in T. reesei regulates many important processes that allow the fungus to recognize, transport, and metabolize different carbon sources during plant cell wall degradation. Nature Publishing Group UK 2018-10-08 /pmc/articles/PMC6175961/ /pubmed/30297963 http://dx.doi.org/10.1038/s41598-018-33383-1 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article de Paula, Renato Graciano Antoniêto, Amanda Cristina Campos Carraro, Cláudia Batista Lopes, Douglas Christian Borges Persinoti, Gabriela Felix Peres, Nalu Teixeira Aguiar Martinez-Rossi, Nilce Maria Silva-Rocha, Rafael Silva, Roberto Nascimento The Duality of the MAPK Signaling Pathway in the Control of Metabolic Processes and Cellulase Production in Trichoderma reesei |
title | The Duality of the MAPK Signaling Pathway in the Control of Metabolic Processes and Cellulase Production in Trichoderma reesei |
title_full | The Duality of the MAPK Signaling Pathway in the Control of Metabolic Processes and Cellulase Production in Trichoderma reesei |
title_fullStr | The Duality of the MAPK Signaling Pathway in the Control of Metabolic Processes and Cellulase Production in Trichoderma reesei |
title_full_unstemmed | The Duality of the MAPK Signaling Pathway in the Control of Metabolic Processes and Cellulase Production in Trichoderma reesei |
title_short | The Duality of the MAPK Signaling Pathway in the Control of Metabolic Processes and Cellulase Production in Trichoderma reesei |
title_sort | duality of the mapk signaling pathway in the control of metabolic processes and cellulase production in trichoderma reesei |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175961/ https://www.ncbi.nlm.nih.gov/pubmed/30297963 http://dx.doi.org/10.1038/s41598-018-33383-1 |
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