Cargando…
Inert Coats of Magnetic Nanoparticles Prevent Formation of Occlusive Intravascular Co-aggregates With Neutrophil Extracellular Traps
If foreign particles enter the human body, the immune system offers several mechanisms of response. Neutrophils forming the first line of the immune defense either remove pathogens by phagocytosis, inactivate them by degranulation or release of reactive oxygen species or immobilize them by the relea...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6176021/ https://www.ncbi.nlm.nih.gov/pubmed/30333831 http://dx.doi.org/10.3389/fimmu.2018.02266 |
_version_ | 1783361621345697792 |
---|---|
author | Bilyy, Rostyslav Unterweger, Harald Weigel, Bianca Dumych, Tetiana Paryzhak, Solomiya Vovk, Volodymyr Liao, Ziyu Alexiou, Christoph Herrmann, Martin Janko, Christina |
author_facet | Bilyy, Rostyslav Unterweger, Harald Weigel, Bianca Dumych, Tetiana Paryzhak, Solomiya Vovk, Volodymyr Liao, Ziyu Alexiou, Christoph Herrmann, Martin Janko, Christina |
author_sort | Bilyy, Rostyslav |
collection | PubMed |
description | If foreign particles enter the human body, the immune system offers several mechanisms of response. Neutrophils forming the first line of the immune defense either remove pathogens by phagocytosis, inactivate them by degranulation or release of reactive oxygen species or immobilize them by the release of chromatin decorated with the granular proteins from cytoplasm as neutrophil extracellular traps (NETs). Besides viable microbes like fungi, bacteria or viruses, also several sterile inorganic particles including nanoparticles reportedly activate NET formation. The physicochemical nanoparticle characteristics fostering NET formation are still elusive. Here we show that agglomerations of non-stabilized superparamagnetic iron oxide nanoparticles (SPIONs) induce NET formation by isolated human neutrophils, in whole blood experiments under static and dynamic conditions as well as in vivo. Stabilization of nanoparticles with biocompatible layers of either human serum albumin or dextran reduced agglomeration and NET formation by neutrophils. Importantly, this passivation of the SPIONs prevented vascular occlusions in vivo even when magnetically accumulated. We conclude that higher order structures formed during nanoparticle agglomeration primarily trigger NET formation and the formation of SPION-aggregated NET-co-aggregates, whereas colloid-disperse nanoparticles behave inert and are alternatively cleared by phagocytosis. |
format | Online Article Text |
id | pubmed-6176021 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-61760212018-10-17 Inert Coats of Magnetic Nanoparticles Prevent Formation of Occlusive Intravascular Co-aggregates With Neutrophil Extracellular Traps Bilyy, Rostyslav Unterweger, Harald Weigel, Bianca Dumych, Tetiana Paryzhak, Solomiya Vovk, Volodymyr Liao, Ziyu Alexiou, Christoph Herrmann, Martin Janko, Christina Front Immunol Immunology If foreign particles enter the human body, the immune system offers several mechanisms of response. Neutrophils forming the first line of the immune defense either remove pathogens by phagocytosis, inactivate them by degranulation or release of reactive oxygen species or immobilize them by the release of chromatin decorated with the granular proteins from cytoplasm as neutrophil extracellular traps (NETs). Besides viable microbes like fungi, bacteria or viruses, also several sterile inorganic particles including nanoparticles reportedly activate NET formation. The physicochemical nanoparticle characteristics fostering NET formation are still elusive. Here we show that agglomerations of non-stabilized superparamagnetic iron oxide nanoparticles (SPIONs) induce NET formation by isolated human neutrophils, in whole blood experiments under static and dynamic conditions as well as in vivo. Stabilization of nanoparticles with biocompatible layers of either human serum albumin or dextran reduced agglomeration and NET formation by neutrophils. Importantly, this passivation of the SPIONs prevented vascular occlusions in vivo even when magnetically accumulated. We conclude that higher order structures formed during nanoparticle agglomeration primarily trigger NET formation and the formation of SPION-aggregated NET-co-aggregates, whereas colloid-disperse nanoparticles behave inert and are alternatively cleared by phagocytosis. Frontiers Media S.A. 2018-10-02 /pmc/articles/PMC6176021/ /pubmed/30333831 http://dx.doi.org/10.3389/fimmu.2018.02266 Text en Copyright © 2018 Bilyy, Unterweger, Weigel, Dumych, Paryzhak, Vovk, Liao, Alexiou, Herrmann and Janko. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Bilyy, Rostyslav Unterweger, Harald Weigel, Bianca Dumych, Tetiana Paryzhak, Solomiya Vovk, Volodymyr Liao, Ziyu Alexiou, Christoph Herrmann, Martin Janko, Christina Inert Coats of Magnetic Nanoparticles Prevent Formation of Occlusive Intravascular Co-aggregates With Neutrophil Extracellular Traps |
title | Inert Coats of Magnetic Nanoparticles Prevent Formation of Occlusive Intravascular Co-aggregates With Neutrophil Extracellular Traps |
title_full | Inert Coats of Magnetic Nanoparticles Prevent Formation of Occlusive Intravascular Co-aggregates With Neutrophil Extracellular Traps |
title_fullStr | Inert Coats of Magnetic Nanoparticles Prevent Formation of Occlusive Intravascular Co-aggregates With Neutrophil Extracellular Traps |
title_full_unstemmed | Inert Coats of Magnetic Nanoparticles Prevent Formation of Occlusive Intravascular Co-aggregates With Neutrophil Extracellular Traps |
title_short | Inert Coats of Magnetic Nanoparticles Prevent Formation of Occlusive Intravascular Co-aggregates With Neutrophil Extracellular Traps |
title_sort | inert coats of magnetic nanoparticles prevent formation of occlusive intravascular co-aggregates with neutrophil extracellular traps |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6176021/ https://www.ncbi.nlm.nih.gov/pubmed/30333831 http://dx.doi.org/10.3389/fimmu.2018.02266 |
work_keys_str_mv | AT bilyyrostyslav inertcoatsofmagneticnanoparticlespreventformationofocclusiveintravascularcoaggregateswithneutrophilextracellulartraps AT unterwegerharald inertcoatsofmagneticnanoparticlespreventformationofocclusiveintravascularcoaggregateswithneutrophilextracellulartraps AT weigelbianca inertcoatsofmagneticnanoparticlespreventformationofocclusiveintravascularcoaggregateswithneutrophilextracellulartraps AT dumychtetiana inertcoatsofmagneticnanoparticlespreventformationofocclusiveintravascularcoaggregateswithneutrophilextracellulartraps AT paryzhaksolomiya inertcoatsofmagneticnanoparticlespreventformationofocclusiveintravascularcoaggregateswithneutrophilextracellulartraps AT vovkvolodymyr inertcoatsofmagneticnanoparticlespreventformationofocclusiveintravascularcoaggregateswithneutrophilextracellulartraps AT liaoziyu inertcoatsofmagneticnanoparticlespreventformationofocclusiveintravascularcoaggregateswithneutrophilextracellulartraps AT alexiouchristoph inertcoatsofmagneticnanoparticlespreventformationofocclusiveintravascularcoaggregateswithneutrophilextracellulartraps AT herrmannmartin inertcoatsofmagneticnanoparticlespreventformationofocclusiveintravascularcoaggregateswithneutrophilextracellulartraps AT jankochristina inertcoatsofmagneticnanoparticlespreventformationofocclusiveintravascularcoaggregateswithneutrophilextracellulartraps |