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Inert Coats of Magnetic Nanoparticles Prevent Formation of Occlusive Intravascular Co-aggregates With Neutrophil Extracellular Traps

If foreign particles enter the human body, the immune system offers several mechanisms of response. Neutrophils forming the first line of the immune defense either remove pathogens by phagocytosis, inactivate them by degranulation or release of reactive oxygen species or immobilize them by the relea...

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Autores principales: Bilyy, Rostyslav, Unterweger, Harald, Weigel, Bianca, Dumych, Tetiana, Paryzhak, Solomiya, Vovk, Volodymyr, Liao, Ziyu, Alexiou, Christoph, Herrmann, Martin, Janko, Christina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6176021/
https://www.ncbi.nlm.nih.gov/pubmed/30333831
http://dx.doi.org/10.3389/fimmu.2018.02266
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author Bilyy, Rostyslav
Unterweger, Harald
Weigel, Bianca
Dumych, Tetiana
Paryzhak, Solomiya
Vovk, Volodymyr
Liao, Ziyu
Alexiou, Christoph
Herrmann, Martin
Janko, Christina
author_facet Bilyy, Rostyslav
Unterweger, Harald
Weigel, Bianca
Dumych, Tetiana
Paryzhak, Solomiya
Vovk, Volodymyr
Liao, Ziyu
Alexiou, Christoph
Herrmann, Martin
Janko, Christina
author_sort Bilyy, Rostyslav
collection PubMed
description If foreign particles enter the human body, the immune system offers several mechanisms of response. Neutrophils forming the first line of the immune defense either remove pathogens by phagocytosis, inactivate them by degranulation or release of reactive oxygen species or immobilize them by the release of chromatin decorated with the granular proteins from cytoplasm as neutrophil extracellular traps (NETs). Besides viable microbes like fungi, bacteria or viruses, also several sterile inorganic particles including nanoparticles reportedly activate NET formation. The physicochemical nanoparticle characteristics fostering NET formation are still elusive. Here we show that agglomerations of non-stabilized superparamagnetic iron oxide nanoparticles (SPIONs) induce NET formation by isolated human neutrophils, in whole blood experiments under static and dynamic conditions as well as in vivo. Stabilization of nanoparticles with biocompatible layers of either human serum albumin or dextran reduced agglomeration and NET formation by neutrophils. Importantly, this passivation of the SPIONs prevented vascular occlusions in vivo even when magnetically accumulated. We conclude that higher order structures formed during nanoparticle agglomeration primarily trigger NET formation and the formation of SPION-aggregated NET-co-aggregates, whereas colloid-disperse nanoparticles behave inert and are alternatively cleared by phagocytosis.
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spelling pubmed-61760212018-10-17 Inert Coats of Magnetic Nanoparticles Prevent Formation of Occlusive Intravascular Co-aggregates With Neutrophil Extracellular Traps Bilyy, Rostyslav Unterweger, Harald Weigel, Bianca Dumych, Tetiana Paryzhak, Solomiya Vovk, Volodymyr Liao, Ziyu Alexiou, Christoph Herrmann, Martin Janko, Christina Front Immunol Immunology If foreign particles enter the human body, the immune system offers several mechanisms of response. Neutrophils forming the first line of the immune defense either remove pathogens by phagocytosis, inactivate them by degranulation or release of reactive oxygen species or immobilize them by the release of chromatin decorated with the granular proteins from cytoplasm as neutrophil extracellular traps (NETs). Besides viable microbes like fungi, bacteria or viruses, also several sterile inorganic particles including nanoparticles reportedly activate NET formation. The physicochemical nanoparticle characteristics fostering NET formation are still elusive. Here we show that agglomerations of non-stabilized superparamagnetic iron oxide nanoparticles (SPIONs) induce NET formation by isolated human neutrophils, in whole blood experiments under static and dynamic conditions as well as in vivo. Stabilization of nanoparticles with biocompatible layers of either human serum albumin or dextran reduced agglomeration and NET formation by neutrophils. Importantly, this passivation of the SPIONs prevented vascular occlusions in vivo even when magnetically accumulated. We conclude that higher order structures formed during nanoparticle agglomeration primarily trigger NET formation and the formation of SPION-aggregated NET-co-aggregates, whereas colloid-disperse nanoparticles behave inert and are alternatively cleared by phagocytosis. Frontiers Media S.A. 2018-10-02 /pmc/articles/PMC6176021/ /pubmed/30333831 http://dx.doi.org/10.3389/fimmu.2018.02266 Text en Copyright © 2018 Bilyy, Unterweger, Weigel, Dumych, Paryzhak, Vovk, Liao, Alexiou, Herrmann and Janko. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Bilyy, Rostyslav
Unterweger, Harald
Weigel, Bianca
Dumych, Tetiana
Paryzhak, Solomiya
Vovk, Volodymyr
Liao, Ziyu
Alexiou, Christoph
Herrmann, Martin
Janko, Christina
Inert Coats of Magnetic Nanoparticles Prevent Formation of Occlusive Intravascular Co-aggregates With Neutrophil Extracellular Traps
title Inert Coats of Magnetic Nanoparticles Prevent Formation of Occlusive Intravascular Co-aggregates With Neutrophil Extracellular Traps
title_full Inert Coats of Magnetic Nanoparticles Prevent Formation of Occlusive Intravascular Co-aggregates With Neutrophil Extracellular Traps
title_fullStr Inert Coats of Magnetic Nanoparticles Prevent Formation of Occlusive Intravascular Co-aggregates With Neutrophil Extracellular Traps
title_full_unstemmed Inert Coats of Magnetic Nanoparticles Prevent Formation of Occlusive Intravascular Co-aggregates With Neutrophil Extracellular Traps
title_short Inert Coats of Magnetic Nanoparticles Prevent Formation of Occlusive Intravascular Co-aggregates With Neutrophil Extracellular Traps
title_sort inert coats of magnetic nanoparticles prevent formation of occlusive intravascular co-aggregates with neutrophil extracellular traps
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6176021/
https://www.ncbi.nlm.nih.gov/pubmed/30333831
http://dx.doi.org/10.3389/fimmu.2018.02266
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