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Eupatilin inhibits microglia activation and attenuates brain injury in intracerebral hemorrhage

Inflammation serves a critical role in the pathophysiology of intracerebral hemorrhage (ICH)-induced brain injury. Eupatilin, a pharmacologically active flavone derived from Artemisia sp., has been reported to have antioxidant, anti-inflammatory, anti-allergic and antitumor activities. However, the...

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Autores principales: Qiao, Hai-Bo, Li, Jia, Lv, Lian-Jie, Nie, Ben-Jin, Lu, Peng, Xue, Feng, Zhang, Zhi-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6176204/
https://www.ncbi.nlm.nih.gov/pubmed/30344678
http://dx.doi.org/10.3892/etm.2018.6699
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author Qiao, Hai-Bo
Li, Jia
Lv, Lian-Jie
Nie, Ben-Jin
Lu, Peng
Xue, Feng
Zhang, Zhi-Ming
author_facet Qiao, Hai-Bo
Li, Jia
Lv, Lian-Jie
Nie, Ben-Jin
Lu, Peng
Xue, Feng
Zhang, Zhi-Ming
author_sort Qiao, Hai-Bo
collection PubMed
description Inflammation serves a critical role in the pathophysiology of intracerebral hemorrhage (ICH)-induced brain injury. Eupatilin, a pharmacologically active flavone derived from Artemisia sp., has been reported to have antioxidant, anti-inflammatory, anti-allergic and antitumor activities. However, the effect of eupatilin in ICH has not been well studied. The aim of the present study was to investigate the effect of eupatilin on ICH-induced microglial inflammation. The MTT and Transwell migration assay results revealed that eupatilin significantly inhibited microglial migration. It also decreased the production of inflammatory cytokines in erythrocyte lysis-induced BV2 cells, as well as the level of intracellular reactive oxygen species. The anti-inflammatory mechanism of eupatilin was also investigated using ELISAs and western blotting and the results demonstrated that eupatilin was able to inhibit erythrocyte lysis-induced NF-κB activation in BV2 cells. Taken together, the results of the present study suggest that eupatilin serves neurological protective effects via inhibiting microglial inflammation, providing an experimental basis for the use of eupatilin as a therapeutic target for ICH.
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spelling pubmed-61762042018-10-21 Eupatilin inhibits microglia activation and attenuates brain injury in intracerebral hemorrhage Qiao, Hai-Bo Li, Jia Lv, Lian-Jie Nie, Ben-Jin Lu, Peng Xue, Feng Zhang, Zhi-Ming Exp Ther Med Articles Inflammation serves a critical role in the pathophysiology of intracerebral hemorrhage (ICH)-induced brain injury. Eupatilin, a pharmacologically active flavone derived from Artemisia sp., has been reported to have antioxidant, anti-inflammatory, anti-allergic and antitumor activities. However, the effect of eupatilin in ICH has not been well studied. The aim of the present study was to investigate the effect of eupatilin on ICH-induced microglial inflammation. The MTT and Transwell migration assay results revealed that eupatilin significantly inhibited microglial migration. It also decreased the production of inflammatory cytokines in erythrocyte lysis-induced BV2 cells, as well as the level of intracellular reactive oxygen species. The anti-inflammatory mechanism of eupatilin was also investigated using ELISAs and western blotting and the results demonstrated that eupatilin was able to inhibit erythrocyte lysis-induced NF-κB activation in BV2 cells. Taken together, the results of the present study suggest that eupatilin serves neurological protective effects via inhibiting microglial inflammation, providing an experimental basis for the use of eupatilin as a therapeutic target for ICH. D.A. Spandidos 2018-11 2018-09-05 /pmc/articles/PMC6176204/ /pubmed/30344678 http://dx.doi.org/10.3892/etm.2018.6699 Text en Copyright: © Qiao et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Qiao, Hai-Bo
Li, Jia
Lv, Lian-Jie
Nie, Ben-Jin
Lu, Peng
Xue, Feng
Zhang, Zhi-Ming
Eupatilin inhibits microglia activation and attenuates brain injury in intracerebral hemorrhage
title Eupatilin inhibits microglia activation and attenuates brain injury in intracerebral hemorrhage
title_full Eupatilin inhibits microglia activation and attenuates brain injury in intracerebral hemorrhage
title_fullStr Eupatilin inhibits microglia activation and attenuates brain injury in intracerebral hemorrhage
title_full_unstemmed Eupatilin inhibits microglia activation and attenuates brain injury in intracerebral hemorrhage
title_short Eupatilin inhibits microglia activation and attenuates brain injury in intracerebral hemorrhage
title_sort eupatilin inhibits microglia activation and attenuates brain injury in intracerebral hemorrhage
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6176204/
https://www.ncbi.nlm.nih.gov/pubmed/30344678
http://dx.doi.org/10.3892/etm.2018.6699
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