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Apatinib suppresses cell growth and metastasis and promotes antitumor activity of temozolomide in glioma
Apatinib, a small-molecule multi-targeted tyrosine kinase inhibitor, is widely used to treat various types of solid tumors. In the present study, it was investigated whether apatinib has therapeutic potential for glioma. Cell Counting Kit-8 and colony formation assays were utilized to determine the...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6176256/ https://www.ncbi.nlm.nih.gov/pubmed/30344715 http://dx.doi.org/10.3892/ol.2018.9355 |
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author | Wang, Chao Jiang, Man Hou, Helei Lin, Qiang Yan, Zhiyong Zhang, Xiaochun |
author_facet | Wang, Chao Jiang, Man Hou, Helei Lin, Qiang Yan, Zhiyong Zhang, Xiaochun |
author_sort | Wang, Chao |
collection | PubMed |
description | Apatinib, a small-molecule multi-targeted tyrosine kinase inhibitor, is widely used to treat various types of solid tumors. In the present study, it was investigated whether apatinib has therapeutic potential for glioma. Cell Counting Kit-8 and colony formation assays were utilized to determine the cell viability of p53- and EGFR-mutated U251MG glioma cells, and wild-type U-87MG ATCC glioma cells. Furthermore, apoptosis, and the invasion and migration abilities of glioma cells were investigated by flow cytometry, invasion assays and wound-healing assays. The potential of the combination of apatinib with temozolomide (TMZ) for glioma therapy was also investigated. The results demonstrate that apatinib significantly inhibited cell proliferation and colony formation through promoting cell apoptosis in p53- and EGFR-mutated and wild-type glioma cells. Cell invasion and migration abilities were notably decreased following treatment with apatinib. Overall, the present study indicates a synergistic antitumor effect of apatinib and TMZ in glioma, and presents a basis for the use of apatinib in glioma treatment. |
format | Online Article Text |
id | pubmed-6176256 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-61762562018-10-21 Apatinib suppresses cell growth and metastasis and promotes antitumor activity of temozolomide in glioma Wang, Chao Jiang, Man Hou, Helei Lin, Qiang Yan, Zhiyong Zhang, Xiaochun Oncol Lett Articles Apatinib, a small-molecule multi-targeted tyrosine kinase inhibitor, is widely used to treat various types of solid tumors. In the present study, it was investigated whether apatinib has therapeutic potential for glioma. Cell Counting Kit-8 and colony formation assays were utilized to determine the cell viability of p53- and EGFR-mutated U251MG glioma cells, and wild-type U-87MG ATCC glioma cells. Furthermore, apoptosis, and the invasion and migration abilities of glioma cells were investigated by flow cytometry, invasion assays and wound-healing assays. The potential of the combination of apatinib with temozolomide (TMZ) for glioma therapy was also investigated. The results demonstrate that apatinib significantly inhibited cell proliferation and colony formation through promoting cell apoptosis in p53- and EGFR-mutated and wild-type glioma cells. Cell invasion and migration abilities were notably decreased following treatment with apatinib. Overall, the present study indicates a synergistic antitumor effect of apatinib and TMZ in glioma, and presents a basis for the use of apatinib in glioma treatment. D.A. Spandidos 2018-11 2018-08-23 /pmc/articles/PMC6176256/ /pubmed/30344715 http://dx.doi.org/10.3892/ol.2018.9355 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Wang, Chao Jiang, Man Hou, Helei Lin, Qiang Yan, Zhiyong Zhang, Xiaochun Apatinib suppresses cell growth and metastasis and promotes antitumor activity of temozolomide in glioma |
title | Apatinib suppresses cell growth and metastasis and promotes antitumor activity of temozolomide in glioma |
title_full | Apatinib suppresses cell growth and metastasis and promotes antitumor activity of temozolomide in glioma |
title_fullStr | Apatinib suppresses cell growth and metastasis and promotes antitumor activity of temozolomide in glioma |
title_full_unstemmed | Apatinib suppresses cell growth and metastasis and promotes antitumor activity of temozolomide in glioma |
title_short | Apatinib suppresses cell growth and metastasis and promotes antitumor activity of temozolomide in glioma |
title_sort | apatinib suppresses cell growth and metastasis and promotes antitumor activity of temozolomide in glioma |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6176256/ https://www.ncbi.nlm.nih.gov/pubmed/30344715 http://dx.doi.org/10.3892/ol.2018.9355 |
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