Cargando…
PAR4 overexpression promotes colorectal cancer cell proliferation and migration
Protease-activated receptor 4 (PAR4), a member of the G-protein-coupled receptor family, was previously identified to be involved in the progression of cancer. Previous study revealed that the expression of PAR4 was increased in colorectal cancer tissues compared with the associated normal tissues,...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6176407/ https://www.ncbi.nlm.nih.gov/pubmed/30333860 http://dx.doi.org/10.3892/ol.2018.9407 |
_version_ | 1783361696210878464 |
---|---|
author | Zhang, Hongshan Jiang, Ping Zhang, Chuanrao Lee, Siman Wang, Wei Zou, Hao |
author_facet | Zhang, Hongshan Jiang, Ping Zhang, Chuanrao Lee, Siman Wang, Wei Zou, Hao |
author_sort | Zhang, Hongshan |
collection | PubMed |
description | Protease-activated receptor 4 (PAR4), a member of the G-protein-coupled receptor family, was previously identified to be involved in the progression of cancer. Previous study revealed that the expression of PAR4 was increased in colorectal cancer tissues compared with the associated normal tissues, particularly in positive lymph node and poorly differentiated types of cancer. We hypothesized that PAR4 serves a function in the progression of colorectal cancer. In the present study, overexpression of PAR4 in colorectal cancer LoVo cells promoted proliferation, anchorage-independent growth and migration. In vivo, PAR4 increased LoVo cell tumorgenicity. In contrast, knockdown of PAR4 in HT-29 cells decreased proliferation, anchorage-independent growth and migration. Mechanistic studies revealed that PAR4 increased the phosphorylation of extracellular-signal-regulated kinase 1/2 in colorectal cancer cells, which is the potential molecular mechanism that promotes cellular proliferation and migration. Taken together, the results of the present study indicated that overexpression of PAR4 promoted colorectal cancer cell proliferation, survival and metastasis, indicating that PAR4 is a promising therapeutic target for preventing colon cancer progression. |
format | Online Article Text |
id | pubmed-6176407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-61764072018-10-17 PAR4 overexpression promotes colorectal cancer cell proliferation and migration Zhang, Hongshan Jiang, Ping Zhang, Chuanrao Lee, Siman Wang, Wei Zou, Hao Oncol Lett Articles Protease-activated receptor 4 (PAR4), a member of the G-protein-coupled receptor family, was previously identified to be involved in the progression of cancer. Previous study revealed that the expression of PAR4 was increased in colorectal cancer tissues compared with the associated normal tissues, particularly in positive lymph node and poorly differentiated types of cancer. We hypothesized that PAR4 serves a function in the progression of colorectal cancer. In the present study, overexpression of PAR4 in colorectal cancer LoVo cells promoted proliferation, anchorage-independent growth and migration. In vivo, PAR4 increased LoVo cell tumorgenicity. In contrast, knockdown of PAR4 in HT-29 cells decreased proliferation, anchorage-independent growth and migration. Mechanistic studies revealed that PAR4 increased the phosphorylation of extracellular-signal-regulated kinase 1/2 in colorectal cancer cells, which is the potential molecular mechanism that promotes cellular proliferation and migration. Taken together, the results of the present study indicated that overexpression of PAR4 promoted colorectal cancer cell proliferation, survival and metastasis, indicating that PAR4 is a promising therapeutic target for preventing colon cancer progression. D.A. Spandidos 2018-11 2018-09-05 /pmc/articles/PMC6176407/ /pubmed/30333860 http://dx.doi.org/10.3892/ol.2018.9407 Text en Copyright: © Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zhang, Hongshan Jiang, Ping Zhang, Chuanrao Lee, Siman Wang, Wei Zou, Hao PAR4 overexpression promotes colorectal cancer cell proliferation and migration |
title | PAR4 overexpression promotes colorectal cancer cell proliferation and migration |
title_full | PAR4 overexpression promotes colorectal cancer cell proliferation and migration |
title_fullStr | PAR4 overexpression promotes colorectal cancer cell proliferation and migration |
title_full_unstemmed | PAR4 overexpression promotes colorectal cancer cell proliferation and migration |
title_short | PAR4 overexpression promotes colorectal cancer cell proliferation and migration |
title_sort | par4 overexpression promotes colorectal cancer cell proliferation and migration |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6176407/ https://www.ncbi.nlm.nih.gov/pubmed/30333860 http://dx.doi.org/10.3892/ol.2018.9407 |
work_keys_str_mv | AT zhanghongshan par4overexpressionpromotescolorectalcancercellproliferationandmigration AT jiangping par4overexpressionpromotescolorectalcancercellproliferationandmigration AT zhangchuanrao par4overexpressionpromotescolorectalcancercellproliferationandmigration AT leesiman par4overexpressionpromotescolorectalcancercellproliferationandmigration AT wangwei par4overexpressionpromotescolorectalcancercellproliferationandmigration AT zouhao par4overexpressionpromotescolorectalcancercellproliferationandmigration |