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CD47-dependent immunomodulatory and angiogenic activities of extracellular vesicles produced by T cells

Intercellular communication is critical for integrating complex signals in multicellular eukaryotes. Vascular endothelial cells and T lymphocytes closely interact during the recirculation and trans-endothelial migration of T cells. In addition to direct cell–cell contact, we show that T cell derived...

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Autores principales: Kaur, Sukhbir, Singh, Satya P., Elkahloun, Abdel G., Wu, Weiwei, Abu-Asab, Mones S., Roberts, David D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6176487/
https://www.ncbi.nlm.nih.gov/pubmed/24887393
http://dx.doi.org/10.1016/j.matbio.2014.05.007
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author Kaur, Sukhbir
Singh, Satya P.
Elkahloun, Abdel G.
Wu, Weiwei
Abu-Asab, Mones S.
Roberts, David D.
author_facet Kaur, Sukhbir
Singh, Satya P.
Elkahloun, Abdel G.
Wu, Weiwei
Abu-Asab, Mones S.
Roberts, David D.
author_sort Kaur, Sukhbir
collection PubMed
description Intercellular communication is critical for integrating complex signals in multicellular eukaryotes. Vascular endothelial cells and T lymphocytes closely interact during the recirculation and trans-endothelial migration of T cells. In addition to direct cell–cell contact, we show that T cell derived extracellular vesicles can interact with endothelial cells and modulate their cellular functions. Thrombospondin-1 and its receptor CD47 are expressed on exosomes/ectosomes derived from T cells, and these extracellular vesicles are internalized and modulate signaling in both T cells and endothelial cells. Extracellular vesicles released from cells expressing or lacking CD47 differentially regulate activation of T cells induced by engaging the T cell receptor. Similarly, T cell-derived extracellular vesicles modulate endothelial cell responses to vascular endothelial growth factor and tube formation in a CD47-dependent manner. Uptake of T cell derived extracellular vesicles by recipient endothelial cells globally alters gene expression in a CD47-dependent manner. CD47 also regulates the mRNA content of extracellular vesicles in a manner consistent with some of the resulting alterations in target endothelial cell gene expression. Therefore, the thrombospondin-1 receptor CD47 directly or indirectly regulates intercellular communication mediated by the transfer of extracellular vesicles between vascular cells.
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spelling pubmed-61764872018-10-09 CD47-dependent immunomodulatory and angiogenic activities of extracellular vesicles produced by T cells Kaur, Sukhbir Singh, Satya P. Elkahloun, Abdel G. Wu, Weiwei Abu-Asab, Mones S. Roberts, David D. Matrix Biol Article Intercellular communication is critical for integrating complex signals in multicellular eukaryotes. Vascular endothelial cells and T lymphocytes closely interact during the recirculation and trans-endothelial migration of T cells. In addition to direct cell–cell contact, we show that T cell derived extracellular vesicles can interact with endothelial cells and modulate their cellular functions. Thrombospondin-1 and its receptor CD47 are expressed on exosomes/ectosomes derived from T cells, and these extracellular vesicles are internalized and modulate signaling in both T cells and endothelial cells. Extracellular vesicles released from cells expressing or lacking CD47 differentially regulate activation of T cells induced by engaging the T cell receptor. Similarly, T cell-derived extracellular vesicles modulate endothelial cell responses to vascular endothelial growth factor and tube formation in a CD47-dependent manner. Uptake of T cell derived extracellular vesicles by recipient endothelial cells globally alters gene expression in a CD47-dependent manner. CD47 also regulates the mRNA content of extracellular vesicles in a manner consistent with some of the resulting alterations in target endothelial cell gene expression. Therefore, the thrombospondin-1 receptor CD47 directly or indirectly regulates intercellular communication mediated by the transfer of extracellular vesicles between vascular cells. 2014-06-02 2014-07 /pmc/articles/PMC6176487/ /pubmed/24887393 http://dx.doi.org/10.1016/j.matbio.2014.05.007 Text en This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Article
Kaur, Sukhbir
Singh, Satya P.
Elkahloun, Abdel G.
Wu, Weiwei
Abu-Asab, Mones S.
Roberts, David D.
CD47-dependent immunomodulatory and angiogenic activities of extracellular vesicles produced by T cells
title CD47-dependent immunomodulatory and angiogenic activities of extracellular vesicles produced by T cells
title_full CD47-dependent immunomodulatory and angiogenic activities of extracellular vesicles produced by T cells
title_fullStr CD47-dependent immunomodulatory and angiogenic activities of extracellular vesicles produced by T cells
title_full_unstemmed CD47-dependent immunomodulatory and angiogenic activities of extracellular vesicles produced by T cells
title_short CD47-dependent immunomodulatory and angiogenic activities of extracellular vesicles produced by T cells
title_sort cd47-dependent immunomodulatory and angiogenic activities of extracellular vesicles produced by t cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6176487/
https://www.ncbi.nlm.nih.gov/pubmed/24887393
http://dx.doi.org/10.1016/j.matbio.2014.05.007
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