The class I myosin MYO1D binds to lipid and protects against colitis

Myosin ID (MYO1D) is a member of the class I myosin family. We screened 48,649 third generation (G3) germline mutant mice derived from N-ethyl-N-nitrosourea-mutagenized grandsires for intestinal homeostasis abnormalities after oral administration of dextran sodium sulfate (DSS). We found and validat...

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Detalles Bibliográficos
Autores principales: McAlpine, William, Wang, Kuan-wen, Choi, Jin Huk, San Miguel, Miguel, McAlpine, Sarah Grace, Russell, Jamie, Ludwig, Sara, Li, Xiaohong, Tang, Miao, Zhan, Xiaoming, Choi, Mihwa, Wang, Tao, Bu, Chun Hui, Murray, Anne R., Moresco, Eva Marie Y., Turer, Emre E., Beutler, Bruce
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6176994/
https://www.ncbi.nlm.nih.gov/pubmed/30279225
http://dx.doi.org/10.1242/dmm.035923
Descripción
Sumario:Myosin ID (MYO1D) is a member of the class I myosin family. We screened 48,649 third generation (G3) germline mutant mice derived from N-ethyl-N-nitrosourea-mutagenized grandsires for intestinal homeostasis abnormalities after oral administration of dextran sodium sulfate (DSS). We found and validated mutations in Myo1d as a cause of increased susceptibility to DSS-induced colitis. MYO1D is produced in the intestinal epithelium, and the colitis phenotype is dependent on the nonhematopoietic compartment of the mouse. Moreover, MYO1D appears to couple cytoskeletal elements to lipid in an ATP-dependent manner. These findings demonstrate that MYO1D is needed to maintain epithelial integrity and protect against DSS-induced colitis.

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