Cargando…
A Langendorff-like system to quantify cardiac pump function in adult zebrafish
Zebrafish are increasingly used as a vertebrate model to study human cardiovascular disorders. Although heart structure and function are readily visualized in zebrafish embryos because of their optical transparency, the lack of effective tools for evaluating the hearts of older, nontransparent fish...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6177000/ https://www.ncbi.nlm.nih.gov/pubmed/30012855 http://dx.doi.org/10.1242/dmm.034819 |
_version_ | 1783361784888950784 |
---|---|
author | Zhang, Hong Dvornikov, Alexey V. Huttner, Inken G. Ma, Xiao Santiago, Celine F. Fatkin, Diane Xu, Xiaolei |
author_facet | Zhang, Hong Dvornikov, Alexey V. Huttner, Inken G. Ma, Xiao Santiago, Celine F. Fatkin, Diane Xu, Xiaolei |
author_sort | Zhang, Hong |
collection | PubMed |
description | Zebrafish are increasingly used as a vertebrate model to study human cardiovascular disorders. Although heart structure and function are readily visualized in zebrafish embryos because of their optical transparency, the lack of effective tools for evaluating the hearts of older, nontransparent fish has been a major limiting factor. The recent development of high-frequency echocardiography has been an important advance for in vivo cardiac assessment, but it necessitates anesthesia and has limited ability to study acute interventions. We report the development of an alternative experimental ex vivo technique for quantifying heart size and function that resembles the Langendorff heart preparations that have been widely used in mammalian models. Dissected adult zebrafish hearts were perfused with a calcium-containing buffer, and a beat frequency was maintained with electrical stimulation. The impact of pacing frequency, flow rate and perfusate calcium concentration on ventricular performance (including end-diastolic and end-systolic volumes, ejection fraction, radial strain, and maximal velocities of shortening and relaxation) were evaluated and optimal conditions defined. We determined the effects of age on heart function in wild-type male and female zebrafish, and successfully detected hypercontractile and hypocontractile responses after adrenergic stimulation or doxorubicin treatment, respectively. Good correlations were found between indices of cardiac contractility obtained with high-frequency echocardiography and with the ex vivo technique in a subset of fish studied with both methods. The ex vivo beating heart preparation is a valuable addition to the cardiac function tool kit that will expand the use of adult zebrafish for cardiovascular research. |
format | Online Article Text |
id | pubmed-6177000 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-61770002018-10-16 A Langendorff-like system to quantify cardiac pump function in adult zebrafish Zhang, Hong Dvornikov, Alexey V. Huttner, Inken G. Ma, Xiao Santiago, Celine F. Fatkin, Diane Xu, Xiaolei Dis Model Mech Resource Article Zebrafish are increasingly used as a vertebrate model to study human cardiovascular disorders. Although heart structure and function are readily visualized in zebrafish embryos because of their optical transparency, the lack of effective tools for evaluating the hearts of older, nontransparent fish has been a major limiting factor. The recent development of high-frequency echocardiography has been an important advance for in vivo cardiac assessment, but it necessitates anesthesia and has limited ability to study acute interventions. We report the development of an alternative experimental ex vivo technique for quantifying heart size and function that resembles the Langendorff heart preparations that have been widely used in mammalian models. Dissected adult zebrafish hearts were perfused with a calcium-containing buffer, and a beat frequency was maintained with electrical stimulation. The impact of pacing frequency, flow rate and perfusate calcium concentration on ventricular performance (including end-diastolic and end-systolic volumes, ejection fraction, radial strain, and maximal velocities of shortening and relaxation) were evaluated and optimal conditions defined. We determined the effects of age on heart function in wild-type male and female zebrafish, and successfully detected hypercontractile and hypocontractile responses after adrenergic stimulation or doxorubicin treatment, respectively. Good correlations were found between indices of cardiac contractility obtained with high-frequency echocardiography and with the ex vivo technique in a subset of fish studied with both methods. The ex vivo beating heart preparation is a valuable addition to the cardiac function tool kit that will expand the use of adult zebrafish for cardiovascular research. The Company of Biologists Ltd 2018-09-01 2018-09-10 /pmc/articles/PMC6177000/ /pubmed/30012855 http://dx.doi.org/10.1242/dmm.034819 Text en © 2018. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Resource Article Zhang, Hong Dvornikov, Alexey V. Huttner, Inken G. Ma, Xiao Santiago, Celine F. Fatkin, Diane Xu, Xiaolei A Langendorff-like system to quantify cardiac pump function in adult zebrafish |
title | A Langendorff-like system to quantify cardiac pump function in adult zebrafish |
title_full | A Langendorff-like system to quantify cardiac pump function in adult zebrafish |
title_fullStr | A Langendorff-like system to quantify cardiac pump function in adult zebrafish |
title_full_unstemmed | A Langendorff-like system to quantify cardiac pump function in adult zebrafish |
title_short | A Langendorff-like system to quantify cardiac pump function in adult zebrafish |
title_sort | langendorff-like system to quantify cardiac pump function in adult zebrafish |
topic | Resource Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6177000/ https://www.ncbi.nlm.nih.gov/pubmed/30012855 http://dx.doi.org/10.1242/dmm.034819 |
work_keys_str_mv | AT zhanghong alangendorfflikesystemtoquantifycardiacpumpfunctioninadultzebrafish AT dvornikovalexeyv alangendorfflikesystemtoquantifycardiacpumpfunctioninadultzebrafish AT huttnerinkeng alangendorfflikesystemtoquantifycardiacpumpfunctioninadultzebrafish AT maxiao alangendorfflikesystemtoquantifycardiacpumpfunctioninadultzebrafish AT santiagocelinef alangendorfflikesystemtoquantifycardiacpumpfunctioninadultzebrafish AT fatkindiane alangendorfflikesystemtoquantifycardiacpumpfunctioninadultzebrafish AT xuxiaolei alangendorfflikesystemtoquantifycardiacpumpfunctioninadultzebrafish AT zhanghong langendorfflikesystemtoquantifycardiacpumpfunctioninadultzebrafish AT dvornikovalexeyv langendorfflikesystemtoquantifycardiacpumpfunctioninadultzebrafish AT huttnerinkeng langendorfflikesystemtoquantifycardiacpumpfunctioninadultzebrafish AT maxiao langendorfflikesystemtoquantifycardiacpumpfunctioninadultzebrafish AT santiagocelinef langendorfflikesystemtoquantifycardiacpumpfunctioninadultzebrafish AT fatkindiane langendorfflikesystemtoquantifycardiacpumpfunctioninadultzebrafish AT xuxiaolei langendorfflikesystemtoquantifycardiacpumpfunctioninadultzebrafish |