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Toxoplasma gondii Manipulates Expression of Host Long Noncoding RNA during Intracellular Infection
Long noncoding RNA (lncRNA) are non-protein-coding transcripts greater than 200 nucleotides that regulate gene expression. The field of transcriptomics is only beginning to understand the role of lncRNA in host defense. Little is known about the role of lncRNA in the response to infection by intrace...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6177471/ https://www.ncbi.nlm.nih.gov/pubmed/30301916 http://dx.doi.org/10.1038/s41598-018-33274-5 |
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author | Menard, Kayla L. Haskins, Breanne E. Colombo, Anthony P. Denkers, Eric Y. |
author_facet | Menard, Kayla L. Haskins, Breanne E. Colombo, Anthony P. Denkers, Eric Y. |
author_sort | Menard, Kayla L. |
collection | PubMed |
description | Long noncoding RNA (lncRNA) are non-protein-coding transcripts greater than 200 nucleotides that regulate gene expression. The field of transcriptomics is only beginning to understand the role of lncRNA in host defense. Little is known about the role of lncRNA in the response to infection by intracellular pathogens such as Toxoplasma gondii. Using a microarray, we examined the differential expression of 35,923 lncRNAs and 24,881 mRNAs in mouse bone-marrow-derived macrophages during infection with high- and low-virulence T. gondii strains. We found that 1,522 lncRNA molecules were differentially regulated during infection with the high-virulence Type I strain, versus 528 with the less-virulent Type II strain. Of these lncRNAs, 282 were co-regulated with a nearby or overlapping mRNA–including approximately 60 mRNAs with immune-related functions. We validated the microarray for 4 lncRNAs and 4 mRNAs using qRT-PCR. Using deletion strains of T. gondii, we found that the secretory kinase ROP16 controls upregulation of lncRNAs Csf1-lnc and Socs2-lnc, demonstrating that the parasite directly manipulates host lncRNA expression. Given the number of regulated lncRNAs and the magnitude of the expression changes, we hypothesize that these molecules constitute both an additional regulatory layer in the host response to infection and a target for manipulation by T. gondii. |
format | Online Article Text |
id | pubmed-6177471 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-61774712018-10-12 Toxoplasma gondii Manipulates Expression of Host Long Noncoding RNA during Intracellular Infection Menard, Kayla L. Haskins, Breanne E. Colombo, Anthony P. Denkers, Eric Y. Sci Rep Article Long noncoding RNA (lncRNA) are non-protein-coding transcripts greater than 200 nucleotides that regulate gene expression. The field of transcriptomics is only beginning to understand the role of lncRNA in host defense. Little is known about the role of lncRNA in the response to infection by intracellular pathogens such as Toxoplasma gondii. Using a microarray, we examined the differential expression of 35,923 lncRNAs and 24,881 mRNAs in mouse bone-marrow-derived macrophages during infection with high- and low-virulence T. gondii strains. We found that 1,522 lncRNA molecules were differentially regulated during infection with the high-virulence Type I strain, versus 528 with the less-virulent Type II strain. Of these lncRNAs, 282 were co-regulated with a nearby or overlapping mRNA–including approximately 60 mRNAs with immune-related functions. We validated the microarray for 4 lncRNAs and 4 mRNAs using qRT-PCR. Using deletion strains of T. gondii, we found that the secretory kinase ROP16 controls upregulation of lncRNAs Csf1-lnc and Socs2-lnc, demonstrating that the parasite directly manipulates host lncRNA expression. Given the number of regulated lncRNAs and the magnitude of the expression changes, we hypothesize that these molecules constitute both an additional regulatory layer in the host response to infection and a target for manipulation by T. gondii. Nature Publishing Group UK 2018-10-09 /pmc/articles/PMC6177471/ /pubmed/30301916 http://dx.doi.org/10.1038/s41598-018-33274-5 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Menard, Kayla L. Haskins, Breanne E. Colombo, Anthony P. Denkers, Eric Y. Toxoplasma gondii Manipulates Expression of Host Long Noncoding RNA during Intracellular Infection |
title | Toxoplasma gondii Manipulates Expression of Host Long Noncoding RNA during Intracellular Infection |
title_full | Toxoplasma gondii Manipulates Expression of Host Long Noncoding RNA during Intracellular Infection |
title_fullStr | Toxoplasma gondii Manipulates Expression of Host Long Noncoding RNA during Intracellular Infection |
title_full_unstemmed | Toxoplasma gondii Manipulates Expression of Host Long Noncoding RNA during Intracellular Infection |
title_short | Toxoplasma gondii Manipulates Expression of Host Long Noncoding RNA during Intracellular Infection |
title_sort | toxoplasma gondii manipulates expression of host long noncoding rna during intracellular infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6177471/ https://www.ncbi.nlm.nih.gov/pubmed/30301916 http://dx.doi.org/10.1038/s41598-018-33274-5 |
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