Cargando…

96-well plate-based aggregometry

While there are many bench and bedside tests to assess platelet reactivity, ex vivo light transmission aggregometry (LTA) remains the gold standard. LTA, however, is expensive, time-consuming and requires dedicated equipment and staff, making it impractical in many situations. In addition, there is...

Descripción completa

Detalles Bibliográficos
Autores principales: Chan, Melissa V., Armstrong, Paul C., Warner, Timothy D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6178088/
https://www.ncbi.nlm.nih.gov/pubmed/29543546
http://dx.doi.org/10.1080/09537104.2018.1445838
_version_ 1783361905445830656
author Chan, Melissa V.
Armstrong, Paul C.
Warner, Timothy D.
author_facet Chan, Melissa V.
Armstrong, Paul C.
Warner, Timothy D.
author_sort Chan, Melissa V.
collection PubMed
description While there are many bench and bedside tests to assess platelet reactivity, ex vivo light transmission aggregometry (LTA) remains the gold standard. LTA, however, is expensive, time-consuming and requires dedicated equipment and staff, making it impractical in many situations. In addition, there is significant variability between data generated at different testing sites meaning that tests often need to be repeated if a patient is transferred to the care of a different hospital. As such, there is clearly an unmet need for standardization of platelet testing. Using the principles of LTA, aggregometry can be conducted in 96-well plates with readings being made in a standard plate reader. This approach allows for the assessment of multiple concentrations of agonists, since the volume of platelets required for each test is significantly lower than for LTA. Furthermore, the lyophilization of a set panel of agonists to a 96-well plate to produce a stable assay substrate allows the production of portable, standardized plates that can be used to generate reproducible tests at multiple sites. In this review, we will discuss the methods and uses of 96-well plate aggregometry for both research and the clinic.
format Online
Article
Text
id pubmed-6178088
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-61780882018-10-22 96-well plate-based aggregometry Chan, Melissa V. Armstrong, Paul C. Warner, Timothy D. Platelets Special Review: Platelets On Aggregometry While there are many bench and bedside tests to assess platelet reactivity, ex vivo light transmission aggregometry (LTA) remains the gold standard. LTA, however, is expensive, time-consuming and requires dedicated equipment and staff, making it impractical in many situations. In addition, there is significant variability between data generated at different testing sites meaning that tests often need to be repeated if a patient is transferred to the care of a different hospital. As such, there is clearly an unmet need for standardization of platelet testing. Using the principles of LTA, aggregometry can be conducted in 96-well plates with readings being made in a standard plate reader. This approach allows for the assessment of multiple concentrations of agonists, since the volume of platelets required for each test is significantly lower than for LTA. Furthermore, the lyophilization of a set panel of agonists to a 96-well plate to produce a stable assay substrate allows the production of portable, standardized plates that can be used to generate reproducible tests at multiple sites. In this review, we will discuss the methods and uses of 96-well plate aggregometry for both research and the clinic. Taylor & Francis 2018-03-15 /pmc/articles/PMC6178088/ /pubmed/29543546 http://dx.doi.org/10.1080/09537104.2018.1445838 Text en © 2018 The Author(s). Published by Taylor & Francis http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Special Review: Platelets On Aggregometry
Chan, Melissa V.
Armstrong, Paul C.
Warner, Timothy D.
96-well plate-based aggregometry
title 96-well plate-based aggregometry
title_full 96-well plate-based aggregometry
title_fullStr 96-well plate-based aggregometry
title_full_unstemmed 96-well plate-based aggregometry
title_short 96-well plate-based aggregometry
title_sort 96-well plate-based aggregometry
topic Special Review: Platelets On Aggregometry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6178088/
https://www.ncbi.nlm.nih.gov/pubmed/29543546
http://dx.doi.org/10.1080/09537104.2018.1445838
work_keys_str_mv AT chanmelissav 96wellplatebasedaggregometry
AT armstrongpaulc 96wellplatebasedaggregometry
AT warnertimothyd 96wellplatebasedaggregometry