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96-well plate-based aggregometry
While there are many bench and bedside tests to assess platelet reactivity, ex vivo light transmission aggregometry (LTA) remains the gold standard. LTA, however, is expensive, time-consuming and requires dedicated equipment and staff, making it impractical in many situations. In addition, there is...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6178088/ https://www.ncbi.nlm.nih.gov/pubmed/29543546 http://dx.doi.org/10.1080/09537104.2018.1445838 |
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author | Chan, Melissa V. Armstrong, Paul C. Warner, Timothy D. |
author_facet | Chan, Melissa V. Armstrong, Paul C. Warner, Timothy D. |
author_sort | Chan, Melissa V. |
collection | PubMed |
description | While there are many bench and bedside tests to assess platelet reactivity, ex vivo light transmission aggregometry (LTA) remains the gold standard. LTA, however, is expensive, time-consuming and requires dedicated equipment and staff, making it impractical in many situations. In addition, there is significant variability between data generated at different testing sites meaning that tests often need to be repeated if a patient is transferred to the care of a different hospital. As such, there is clearly an unmet need for standardization of platelet testing. Using the principles of LTA, aggregometry can be conducted in 96-well plates with readings being made in a standard plate reader. This approach allows for the assessment of multiple concentrations of agonists, since the volume of platelets required for each test is significantly lower than for LTA. Furthermore, the lyophilization of a set panel of agonists to a 96-well plate to produce a stable assay substrate allows the production of portable, standardized plates that can be used to generate reproducible tests at multiple sites. In this review, we will discuss the methods and uses of 96-well plate aggregometry for both research and the clinic. |
format | Online Article Text |
id | pubmed-6178088 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-61780882018-10-22 96-well plate-based aggregometry Chan, Melissa V. Armstrong, Paul C. Warner, Timothy D. Platelets Special Review: Platelets On Aggregometry While there are many bench and bedside tests to assess platelet reactivity, ex vivo light transmission aggregometry (LTA) remains the gold standard. LTA, however, is expensive, time-consuming and requires dedicated equipment and staff, making it impractical in many situations. In addition, there is significant variability between data generated at different testing sites meaning that tests often need to be repeated if a patient is transferred to the care of a different hospital. As such, there is clearly an unmet need for standardization of platelet testing. Using the principles of LTA, aggregometry can be conducted in 96-well plates with readings being made in a standard plate reader. This approach allows for the assessment of multiple concentrations of agonists, since the volume of platelets required for each test is significantly lower than for LTA. Furthermore, the lyophilization of a set panel of agonists to a 96-well plate to produce a stable assay substrate allows the production of portable, standardized plates that can be used to generate reproducible tests at multiple sites. In this review, we will discuss the methods and uses of 96-well plate aggregometry for both research and the clinic. Taylor & Francis 2018-03-15 /pmc/articles/PMC6178088/ /pubmed/29543546 http://dx.doi.org/10.1080/09537104.2018.1445838 Text en © 2018 The Author(s). Published by Taylor & Francis http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Special Review: Platelets On Aggregometry Chan, Melissa V. Armstrong, Paul C. Warner, Timothy D. 96-well plate-based aggregometry |
title | 96-well plate-based aggregometry |
title_full | 96-well plate-based aggregometry |
title_fullStr | 96-well plate-based aggregometry |
title_full_unstemmed | 96-well plate-based aggregometry |
title_short | 96-well plate-based aggregometry |
title_sort | 96-well plate-based aggregometry |
topic | Special Review: Platelets On Aggregometry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6178088/ https://www.ncbi.nlm.nih.gov/pubmed/29543546 http://dx.doi.org/10.1080/09537104.2018.1445838 |
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