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A Highly Pathogenic Strain of Porcine Deltacoronavirus Caused Watery Diarrhea in Newborn Piglets

Porcine deltacoronavirus (PDCoV) is a newly identified virus that causes watery diarrhea in newborn piglets and results in significant economic losses to the pig industry. Since first reported in Hong Kong in 2012, PDCoV has been subsequently detected in USA, South Korea, Thailand, and mainland Chin...

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Autores principales: Xu, Zhichao, Zhong, Huiling, Zhou, Qingfeng, Du, Yunping, Chen, Li, Zhang, Yun, Xue, Chunyi, Cao, Yongchang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Singapore 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6178105/
https://www.ncbi.nlm.nih.gov/pubmed/29569144
http://dx.doi.org/10.1007/s12250-018-0003-8
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author Xu, Zhichao
Zhong, Huiling
Zhou, Qingfeng
Du, Yunping
Chen, Li
Zhang, Yun
Xue, Chunyi
Cao, Yongchang
author_facet Xu, Zhichao
Zhong, Huiling
Zhou, Qingfeng
Du, Yunping
Chen, Li
Zhang, Yun
Xue, Chunyi
Cao, Yongchang
author_sort Xu, Zhichao
collection PubMed
description Porcine deltacoronavirus (PDCoV) is a newly identified virus that causes watery diarrhea in newborn piglets and results in significant economic losses to the pig industry. Since first reported in Hong Kong in 2012, PDCoV has been subsequently detected in USA, South Korea, Thailand, and mainland China. Here we isolated a strain of PDCoV, named CHN-GD-2016, from the intestinal content of a diseased newborn piglet with severe diarrhea in a pig farm in Guangdong, China. PDCoV CHN-GD-2016 could be identified by immunofluorescence with PDCoV specific rabbit antisera, and typical crown-shaped particles with spiky surface projections of this PDCoV were observed with electron microscopy. Genomic analysis showed that the PDCoV CHN-GD-2016 was closely related to other Chinese PDCoV strains, with the highest sequence similarity with the strain CHN/Tianjin/2016. Importantly, inoculation of newborn piglets with 1 × 10(5) TCID(50) of CHN-GD-2016 by oral feeding successfully reproduced clear clinical symptoms, including vomiting, dehydration, and severe diarrhea in piglets. In addition, the virus RNA in rectal swabs from 1 to 7 days post inoculation was detected, macroscopic and microscopic lesions in small intestine were observed, and viral antigen was also detected in the small intestines with immunohistochemical staining. Collectively, the data show in this study confirms that PDCoV is present in Guangdong, China and is highly pathogenic in newborn piglets.
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spelling pubmed-61781052019-03-22 A Highly Pathogenic Strain of Porcine Deltacoronavirus Caused Watery Diarrhea in Newborn Piglets Xu, Zhichao Zhong, Huiling Zhou, Qingfeng Du, Yunping Chen, Li Zhang, Yun Xue, Chunyi Cao, Yongchang Virol Sin Research Article Porcine deltacoronavirus (PDCoV) is a newly identified virus that causes watery diarrhea in newborn piglets and results in significant economic losses to the pig industry. Since first reported in Hong Kong in 2012, PDCoV has been subsequently detected in USA, South Korea, Thailand, and mainland China. Here we isolated a strain of PDCoV, named CHN-GD-2016, from the intestinal content of a diseased newborn piglet with severe diarrhea in a pig farm in Guangdong, China. PDCoV CHN-GD-2016 could be identified by immunofluorescence with PDCoV specific rabbit antisera, and typical crown-shaped particles with spiky surface projections of this PDCoV were observed with electron microscopy. Genomic analysis showed that the PDCoV CHN-GD-2016 was closely related to other Chinese PDCoV strains, with the highest sequence similarity with the strain CHN/Tianjin/2016. Importantly, inoculation of newborn piglets with 1 × 10(5) TCID(50) of CHN-GD-2016 by oral feeding successfully reproduced clear clinical symptoms, including vomiting, dehydration, and severe diarrhea in piglets. In addition, the virus RNA in rectal swabs from 1 to 7 days post inoculation was detected, macroscopic and microscopic lesions in small intestine were observed, and viral antigen was also detected in the small intestines with immunohistochemical staining. Collectively, the data show in this study confirms that PDCoV is present in Guangdong, China and is highly pathogenic in newborn piglets. Springer Singapore 2018-03-22 /pmc/articles/PMC6178105/ /pubmed/29569144 http://dx.doi.org/10.1007/s12250-018-0003-8 Text en © Wuhan Institute of Virology, CAS and Springer Nature Singapore Pte Ltd. 2018
spellingShingle Research Article
Xu, Zhichao
Zhong, Huiling
Zhou, Qingfeng
Du, Yunping
Chen, Li
Zhang, Yun
Xue, Chunyi
Cao, Yongchang
A Highly Pathogenic Strain of Porcine Deltacoronavirus Caused Watery Diarrhea in Newborn Piglets
title A Highly Pathogenic Strain of Porcine Deltacoronavirus Caused Watery Diarrhea in Newborn Piglets
title_full A Highly Pathogenic Strain of Porcine Deltacoronavirus Caused Watery Diarrhea in Newborn Piglets
title_fullStr A Highly Pathogenic Strain of Porcine Deltacoronavirus Caused Watery Diarrhea in Newborn Piglets
title_full_unstemmed A Highly Pathogenic Strain of Porcine Deltacoronavirus Caused Watery Diarrhea in Newborn Piglets
title_short A Highly Pathogenic Strain of Porcine Deltacoronavirus Caused Watery Diarrhea in Newborn Piglets
title_sort highly pathogenic strain of porcine deltacoronavirus caused watery diarrhea in newborn piglets
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6178105/
https://www.ncbi.nlm.nih.gov/pubmed/29569144
http://dx.doi.org/10.1007/s12250-018-0003-8
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