Cargando…

Irisin Exerts Neuroprotective Effects on Cultured Neurons by Regulating Astrocytes

Neurons suffer detrimental effects from β-amyloid toxicity in Alzheimer's disease. The exercise hormone, irisin, is found to induce a neuroprotective gene program and facilitates the beneficial effects on cognitive function. But no effort is made to test its direct protective effects on neurons...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Kexin, Li, Hongyan, Wang, Hongxing, Wang, Jun-hui, Song, Feng, Sun, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6178172/
https://www.ncbi.nlm.nih.gov/pubmed/30356412
http://dx.doi.org/10.1155/2018/9070341
_version_ 1783361919188467712
author Wang, Kexin
Li, Hongyan
Wang, Hongxing
Wang, Jun-hui
Song, Feng
Sun, Yu
author_facet Wang, Kexin
Li, Hongyan
Wang, Hongxing
Wang, Jun-hui
Song, Feng
Sun, Yu
author_sort Wang, Kexin
collection PubMed
description Neurons suffer detrimental effects from β-amyloid toxicity in Alzheimer's disease. The exercise hormone, irisin, is found to induce a neuroprotective gene program and facilitates the beneficial effects on cognitive function. But no effort is made to test its direct protective effects on neurons against the Aβ-induced cell toxicity so far. In the present study, we investigated whether irisin could protect neurons against Aβ- (25–35) induced cell damage and explored the possible underlying mechanisms. Primary cell cultures of astrocytes and neurons were established. Conditioned medium from astrocyte was collected for the treatment and biochemistry assay study. To explore the protein expression changes, Western blot and ELISA assays were used in these in vitro cell culture models. Exposure of hippocampal neurons to 10 μM Aβ (25–35) caused significant reduction on cell viability, and the toxic effect was not significantly reduced by the coadministration of irisin. However, pretreated astrocyte-conditioned medium with irisin for 12 hours notably protected the neurons from the toxicity of Aβ. Also, we found that irisin could attenuate the release of IL-6 and IL-1β from cultured astrocytes and decrease the expression level of COX-2 and phosphorylation of AKT. Last, we found that irisin could reduce NFκB activation in astrocyte exposed to Aβ by preventing the phosphorylation and the loss of IκBα. Our finding may provide novel evidence for the future application of irisin in the treatment of Alzheimer's disease and the memory dysfunction in diabetes mellitus.
format Online
Article
Text
id pubmed-6178172
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-61781722018-10-23 Irisin Exerts Neuroprotective Effects on Cultured Neurons by Regulating Astrocytes Wang, Kexin Li, Hongyan Wang, Hongxing Wang, Jun-hui Song, Feng Sun, Yu Mediators Inflamm Research Article Neurons suffer detrimental effects from β-amyloid toxicity in Alzheimer's disease. The exercise hormone, irisin, is found to induce a neuroprotective gene program and facilitates the beneficial effects on cognitive function. But no effort is made to test its direct protective effects on neurons against the Aβ-induced cell toxicity so far. In the present study, we investigated whether irisin could protect neurons against Aβ- (25–35) induced cell damage and explored the possible underlying mechanisms. Primary cell cultures of astrocytes and neurons were established. Conditioned medium from astrocyte was collected for the treatment and biochemistry assay study. To explore the protein expression changes, Western blot and ELISA assays were used in these in vitro cell culture models. Exposure of hippocampal neurons to 10 μM Aβ (25–35) caused significant reduction on cell viability, and the toxic effect was not significantly reduced by the coadministration of irisin. However, pretreated astrocyte-conditioned medium with irisin for 12 hours notably protected the neurons from the toxicity of Aβ. Also, we found that irisin could attenuate the release of IL-6 and IL-1β from cultured astrocytes and decrease the expression level of COX-2 and phosphorylation of AKT. Last, we found that irisin could reduce NFκB activation in astrocyte exposed to Aβ by preventing the phosphorylation and the loss of IκBα. Our finding may provide novel evidence for the future application of irisin in the treatment of Alzheimer's disease and the memory dysfunction in diabetes mellitus. Hindawi 2018-09-26 /pmc/articles/PMC6178172/ /pubmed/30356412 http://dx.doi.org/10.1155/2018/9070341 Text en Copyright © 2018 Kexin Wang et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wang, Kexin
Li, Hongyan
Wang, Hongxing
Wang, Jun-hui
Song, Feng
Sun, Yu
Irisin Exerts Neuroprotective Effects on Cultured Neurons by Regulating Astrocytes
title Irisin Exerts Neuroprotective Effects on Cultured Neurons by Regulating Astrocytes
title_full Irisin Exerts Neuroprotective Effects on Cultured Neurons by Regulating Astrocytes
title_fullStr Irisin Exerts Neuroprotective Effects on Cultured Neurons by Regulating Astrocytes
title_full_unstemmed Irisin Exerts Neuroprotective Effects on Cultured Neurons by Regulating Astrocytes
title_short Irisin Exerts Neuroprotective Effects on Cultured Neurons by Regulating Astrocytes
title_sort irisin exerts neuroprotective effects on cultured neurons by regulating astrocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6178172/
https://www.ncbi.nlm.nih.gov/pubmed/30356412
http://dx.doi.org/10.1155/2018/9070341
work_keys_str_mv AT wangkexin irisinexertsneuroprotectiveeffectsonculturedneuronsbyregulatingastrocytes
AT lihongyan irisinexertsneuroprotectiveeffectsonculturedneuronsbyregulatingastrocytes
AT wanghongxing irisinexertsneuroprotectiveeffectsonculturedneuronsbyregulatingastrocytes
AT wangjunhui irisinexertsneuroprotectiveeffectsonculturedneuronsbyregulatingastrocytes
AT songfeng irisinexertsneuroprotectiveeffectsonculturedneuronsbyregulatingastrocytes
AT sunyu irisinexertsneuroprotectiveeffectsonculturedneuronsbyregulatingastrocytes