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Cell Surface N-Glycans Influence Electrophysiological Properties and Fate Potential of Neural Stem Cells

Understanding the cellular properties controlling neural stem and progenitor cell (NSPC) fate choice will improve their therapeutic potential. The electrophysiological measure whole-cell membrane capacitance reflects fate bias in the neural lineage but the cellular properties underlying membrane cap...

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Autores principales: Yale, Andrew R., Nourse, Jamison L., Lee, Kayla R., Ahmed, Syed N., Arulmoli, Janahan, Jiang, Alan Y.L., McDonnell, Lisa P., Botten, Giovanni A., Lee, Abraham P., Monuki, Edwin S., Demetriou, Michael, Flanagan, Lisa A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6178213/
https://www.ncbi.nlm.nih.gov/pubmed/30197120
http://dx.doi.org/10.1016/j.stemcr.2018.08.011
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author Yale, Andrew R.
Nourse, Jamison L.
Lee, Kayla R.
Ahmed, Syed N.
Arulmoli, Janahan
Jiang, Alan Y.L.
McDonnell, Lisa P.
Botten, Giovanni A.
Lee, Abraham P.
Monuki, Edwin S.
Demetriou, Michael
Flanagan, Lisa A.
author_facet Yale, Andrew R.
Nourse, Jamison L.
Lee, Kayla R.
Ahmed, Syed N.
Arulmoli, Janahan
Jiang, Alan Y.L.
McDonnell, Lisa P.
Botten, Giovanni A.
Lee, Abraham P.
Monuki, Edwin S.
Demetriou, Michael
Flanagan, Lisa A.
author_sort Yale, Andrew R.
collection PubMed
description Understanding the cellular properties controlling neural stem and progenitor cell (NSPC) fate choice will improve their therapeutic potential. The electrophysiological measure whole-cell membrane capacitance reflects fate bias in the neural lineage but the cellular properties underlying membrane capacitance are poorly understood. We tested the hypothesis that cell surface carbohydrates contribute to NSPC membrane capacitance and fate. We found NSPCs differing in fate potential express distinct patterns of glycosylation enzymes. Screening several glycosylation pathways revealed that the one forming highly branched N-glycans differs between neurogenic and astrogenic populations of cells in vitro and in vivo. Enhancing highly branched N-glycans on NSPCs significantly increases membrane capacitance and leads to the generation of more astrocytes at the expense of neurons with no effect on cell size, viability, or proliferation. These data identify the N-glycan branching pathway as a significant regulator of membrane capacitance and fate choice in the neural lineage.
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spelling pubmed-61782132018-10-11 Cell Surface N-Glycans Influence Electrophysiological Properties and Fate Potential of Neural Stem Cells Yale, Andrew R. Nourse, Jamison L. Lee, Kayla R. Ahmed, Syed N. Arulmoli, Janahan Jiang, Alan Y.L. McDonnell, Lisa P. Botten, Giovanni A. Lee, Abraham P. Monuki, Edwin S. Demetriou, Michael Flanagan, Lisa A. Stem Cell Reports Article Understanding the cellular properties controlling neural stem and progenitor cell (NSPC) fate choice will improve their therapeutic potential. The electrophysiological measure whole-cell membrane capacitance reflects fate bias in the neural lineage but the cellular properties underlying membrane capacitance are poorly understood. We tested the hypothesis that cell surface carbohydrates contribute to NSPC membrane capacitance and fate. We found NSPCs differing in fate potential express distinct patterns of glycosylation enzymes. Screening several glycosylation pathways revealed that the one forming highly branched N-glycans differs between neurogenic and astrogenic populations of cells in vitro and in vivo. Enhancing highly branched N-glycans on NSPCs significantly increases membrane capacitance and leads to the generation of more astrocytes at the expense of neurons with no effect on cell size, viability, or proliferation. These data identify the N-glycan branching pathway as a significant regulator of membrane capacitance and fate choice in the neural lineage. Elsevier 2018-09-06 /pmc/articles/PMC6178213/ /pubmed/30197120 http://dx.doi.org/10.1016/j.stemcr.2018.08.011 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Yale, Andrew R.
Nourse, Jamison L.
Lee, Kayla R.
Ahmed, Syed N.
Arulmoli, Janahan
Jiang, Alan Y.L.
McDonnell, Lisa P.
Botten, Giovanni A.
Lee, Abraham P.
Monuki, Edwin S.
Demetriou, Michael
Flanagan, Lisa A.
Cell Surface N-Glycans Influence Electrophysiological Properties and Fate Potential of Neural Stem Cells
title Cell Surface N-Glycans Influence Electrophysiological Properties and Fate Potential of Neural Stem Cells
title_full Cell Surface N-Glycans Influence Electrophysiological Properties and Fate Potential of Neural Stem Cells
title_fullStr Cell Surface N-Glycans Influence Electrophysiological Properties and Fate Potential of Neural Stem Cells
title_full_unstemmed Cell Surface N-Glycans Influence Electrophysiological Properties and Fate Potential of Neural Stem Cells
title_short Cell Surface N-Glycans Influence Electrophysiological Properties and Fate Potential of Neural Stem Cells
title_sort cell surface n-glycans influence electrophysiological properties and fate potential of neural stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6178213/
https://www.ncbi.nlm.nih.gov/pubmed/30197120
http://dx.doi.org/10.1016/j.stemcr.2018.08.011
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