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Systems and Synthetic Biology Approaches to Engineer Fungi for Fine Chemical Production

Since the advent of systems and synthetic biology, many studies have sought to harness microbes as cell factories through genetic and metabolic engineering approaches. Yeast and filamentous fungi have been successfully harnessed to produce fine and high value-added chemical products. In this review,...

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Autores principales: Martins-Santana, Leonardo, Nora, Luisa C., Sanches-Medeiros, Ananda, Lovate, Gabriel L., Cassiano, Murilo H. A., Silva-Rocha, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6178918/
https://www.ncbi.nlm.nih.gov/pubmed/30338257
http://dx.doi.org/10.3389/fbioe.2018.00117
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author Martins-Santana, Leonardo
Nora, Luisa C.
Sanches-Medeiros, Ananda
Lovate, Gabriel L.
Cassiano, Murilo H. A.
Silva-Rocha, Rafael
author_facet Martins-Santana, Leonardo
Nora, Luisa C.
Sanches-Medeiros, Ananda
Lovate, Gabriel L.
Cassiano, Murilo H. A.
Silva-Rocha, Rafael
author_sort Martins-Santana, Leonardo
collection PubMed
description Since the advent of systems and synthetic biology, many studies have sought to harness microbes as cell factories through genetic and metabolic engineering approaches. Yeast and filamentous fungi have been successfully harnessed to produce fine and high value-added chemical products. In this review, we present some of the most promising advances from recent years in the use of fungi for this purpose, focusing on the manipulation of fungal strains using systems and synthetic biology tools to improve metabolic flow and the flow of secondary metabolites by pathway redesign. We also review the roles of bioinformatics analysis and predictions in synthetic circuits, highlighting in silico systemic approaches to improve the efficiency of synthetic modules.
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spelling pubmed-61789182018-10-18 Systems and Synthetic Biology Approaches to Engineer Fungi for Fine Chemical Production Martins-Santana, Leonardo Nora, Luisa C. Sanches-Medeiros, Ananda Lovate, Gabriel L. Cassiano, Murilo H. A. Silva-Rocha, Rafael Front Bioeng Biotechnol Bioengineering and Biotechnology Since the advent of systems and synthetic biology, many studies have sought to harness microbes as cell factories through genetic and metabolic engineering approaches. Yeast and filamentous fungi have been successfully harnessed to produce fine and high value-added chemical products. In this review, we present some of the most promising advances from recent years in the use of fungi for this purpose, focusing on the manipulation of fungal strains using systems and synthetic biology tools to improve metabolic flow and the flow of secondary metabolites by pathway redesign. We also review the roles of bioinformatics analysis and predictions in synthetic circuits, highlighting in silico systemic approaches to improve the efficiency of synthetic modules. Frontiers Media S.A. 2018-10-03 /pmc/articles/PMC6178918/ /pubmed/30338257 http://dx.doi.org/10.3389/fbioe.2018.00117 Text en Copyright © 2018 Martins-Santana, Nora, Sanches-Medeiros, Lovate, Cassiano and Silva-Rocha. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Martins-Santana, Leonardo
Nora, Luisa C.
Sanches-Medeiros, Ananda
Lovate, Gabriel L.
Cassiano, Murilo H. A.
Silva-Rocha, Rafael
Systems and Synthetic Biology Approaches to Engineer Fungi for Fine Chemical Production
title Systems and Synthetic Biology Approaches to Engineer Fungi for Fine Chemical Production
title_full Systems and Synthetic Biology Approaches to Engineer Fungi for Fine Chemical Production
title_fullStr Systems and Synthetic Biology Approaches to Engineer Fungi for Fine Chemical Production
title_full_unstemmed Systems and Synthetic Biology Approaches to Engineer Fungi for Fine Chemical Production
title_short Systems and Synthetic Biology Approaches to Engineer Fungi for Fine Chemical Production
title_sort systems and synthetic biology approaches to engineer fungi for fine chemical production
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6178918/
https://www.ncbi.nlm.nih.gov/pubmed/30338257
http://dx.doi.org/10.3389/fbioe.2018.00117
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