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Dicranopteris linearis extract inhibits the proliferation of human breast cancer cell line (MDA-MB-231) via induction of S-phase arrest and apoptosis

Context:Dicranopteris linearis (Burm.f.) Underw. (Gleicheniaceae) has been scientifically proven to exert various pharmacological activities. Nevertheless, its anti-proliferative potential has not been extensively investigated. Objective: To investigate the anti-proliferative potential of D. lineari...

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Autores principales: Baharuddin, Aifaa Akmal, Roosli, Rushduddin Al Jufri, Zakaria, Zainul Amiruddin, Md. Tohid, Siti Farah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6179048/
https://www.ncbi.nlm.nih.gov/pubmed/30301390
http://dx.doi.org/10.1080/13880209.2018.1495748
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author Baharuddin, Aifaa Akmal
Roosli, Rushduddin Al Jufri
Zakaria, Zainul Amiruddin
Md. Tohid, Siti Farah
author_facet Baharuddin, Aifaa Akmal
Roosli, Rushduddin Al Jufri
Zakaria, Zainul Amiruddin
Md. Tohid, Siti Farah
author_sort Baharuddin, Aifaa Akmal
collection PubMed
description Context:Dicranopteris linearis (Burm.f.) Underw. (Gleicheniaceae) has been scientifically proven to exert various pharmacological activities. Nevertheless, its anti-proliferative potential has not been extensively investigated. Objective: To investigate the anti-proliferative potential of D. linearis leaves and determine possible mechanistic pathways. Materials and methods: MTT assay was used to determine the cytotoxic effects of D. linearis methanol (MEDL) and petroleum ether (PEEDL) extracts at concentrations of 100, 50, 25, 12.5, 6.25 and 3.125 µg/mL against a panel of cancer cell lines (breast [MCF-7 and MDA-MB-231], cervical [HeLa], colon [HT-29], hepatocellular [HepG2] and lung [A549]), as compared to negative (untreated) and positive [5-fluorouracil (5-FU)-treated] control groups. Mouse fibroblast cells (3T3) were used as normal cells. The mode of cell death was examined using morphological analysis via acridine orange (AO) and propidium iodide (PI) double staining. Cell cycle arrest was determined using flow cytometer, followed by annexin V-PI apoptosis detection kit. Results: MEDL demonstrated the most significant growth inhibition against MDA-MB-231 cells (IC(50) 22.4 µg/mL). PEEDL showed no cytotoxic effect. Induction of apoptosis by MEDL was evidenced via morphological analysis and acridine orange propidium iodide staining. MEDL could induce S phase cell cycle arrest after 72 h of incubation. Early apoptosis induction in MDA-MB-231 cells was confirmed by annexin V-FITC and PI staining. Significant increase in apoptotic cells were detected after 24 h of treatment with 15.07% cells underwent apoptosis, and the amount escalated to 18.24% with prolonged 48 h incubation. Conclusions: MEDL has potential as a potent cytotoxic agent against MDA-MB-231 adenocarcinoma.
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spelling pubmed-61790482018-10-12 Dicranopteris linearis extract inhibits the proliferation of human breast cancer cell line (MDA-MB-231) via induction of S-phase arrest and apoptosis Baharuddin, Aifaa Akmal Roosli, Rushduddin Al Jufri Zakaria, Zainul Amiruddin Md. Tohid, Siti Farah Pharm Biol Research Article Context:Dicranopteris linearis (Burm.f.) Underw. (Gleicheniaceae) has been scientifically proven to exert various pharmacological activities. Nevertheless, its anti-proliferative potential has not been extensively investigated. Objective: To investigate the anti-proliferative potential of D. linearis leaves and determine possible mechanistic pathways. Materials and methods: MTT assay was used to determine the cytotoxic effects of D. linearis methanol (MEDL) and petroleum ether (PEEDL) extracts at concentrations of 100, 50, 25, 12.5, 6.25 and 3.125 µg/mL against a panel of cancer cell lines (breast [MCF-7 and MDA-MB-231], cervical [HeLa], colon [HT-29], hepatocellular [HepG2] and lung [A549]), as compared to negative (untreated) and positive [5-fluorouracil (5-FU)-treated] control groups. Mouse fibroblast cells (3T3) were used as normal cells. The mode of cell death was examined using morphological analysis via acridine orange (AO) and propidium iodide (PI) double staining. Cell cycle arrest was determined using flow cytometer, followed by annexin V-PI apoptosis detection kit. Results: MEDL demonstrated the most significant growth inhibition against MDA-MB-231 cells (IC(50) 22.4 µg/mL). PEEDL showed no cytotoxic effect. Induction of apoptosis by MEDL was evidenced via morphological analysis and acridine orange propidium iodide staining. MEDL could induce S phase cell cycle arrest after 72 h of incubation. Early apoptosis induction in MDA-MB-231 cells was confirmed by annexin V-FITC and PI staining. Significant increase in apoptotic cells were detected after 24 h of treatment with 15.07% cells underwent apoptosis, and the amount escalated to 18.24% with prolonged 48 h incubation. Conclusions: MEDL has potential as a potent cytotoxic agent against MDA-MB-231 adenocarcinoma. Taylor & Francis 2018-10-10 /pmc/articles/PMC6179048/ /pubmed/30301390 http://dx.doi.org/10.1080/13880209.2018.1495748 Text en © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Baharuddin, Aifaa Akmal
Roosli, Rushduddin Al Jufri
Zakaria, Zainul Amiruddin
Md. Tohid, Siti Farah
Dicranopteris linearis extract inhibits the proliferation of human breast cancer cell line (MDA-MB-231) via induction of S-phase arrest and apoptosis
title Dicranopteris linearis extract inhibits the proliferation of human breast cancer cell line (MDA-MB-231) via induction of S-phase arrest and apoptosis
title_full Dicranopteris linearis extract inhibits the proliferation of human breast cancer cell line (MDA-MB-231) via induction of S-phase arrest and apoptosis
title_fullStr Dicranopteris linearis extract inhibits the proliferation of human breast cancer cell line (MDA-MB-231) via induction of S-phase arrest and apoptosis
title_full_unstemmed Dicranopteris linearis extract inhibits the proliferation of human breast cancer cell line (MDA-MB-231) via induction of S-phase arrest and apoptosis
title_short Dicranopteris linearis extract inhibits the proliferation of human breast cancer cell line (MDA-MB-231) via induction of S-phase arrest and apoptosis
title_sort dicranopteris linearis extract inhibits the proliferation of human breast cancer cell line (mda-mb-231) via induction of s-phase arrest and apoptosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6179048/
https://www.ncbi.nlm.nih.gov/pubmed/30301390
http://dx.doi.org/10.1080/13880209.2018.1495748
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