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Antimicrobial nano-zinc oxide-2S albumin protein formulation significantly inhibits growth of “Candidatus Liberibacter asiaticus” in planta

Huanglongbing (HLB, also known as citrus greening) is considered to be the most devastating disease that has significantly damaged the citrus industry globally. HLB is caused by the Candidatus Liberibacter asiaticus (CLas), the fastidious phloem-restricted gram-negative bacterium, vectored by the as...

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Autores principales: Ghosh, Dilip Kumar, Kokane, Sunil, Kumar, Pranav, Ozcan, Ali, Warghane, Ashish, Motghare, Manali, Santra, Swadeshmukul, Sharma, Ashwani Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6179220/
https://www.ncbi.nlm.nih.gov/pubmed/30304000
http://dx.doi.org/10.1371/journal.pone.0204702
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author Ghosh, Dilip Kumar
Kokane, Sunil
Kumar, Pranav
Ozcan, Ali
Warghane, Ashish
Motghare, Manali
Santra, Swadeshmukul
Sharma, Ashwani Kumar
author_facet Ghosh, Dilip Kumar
Kokane, Sunil
Kumar, Pranav
Ozcan, Ali
Warghane, Ashish
Motghare, Manali
Santra, Swadeshmukul
Sharma, Ashwani Kumar
author_sort Ghosh, Dilip Kumar
collection PubMed
description Huanglongbing (HLB, also known as citrus greening) is considered to be the most devastating disease that has significantly damaged the citrus industry globally. HLB is caused by the Candidatus Liberibacter asiaticus (CLas), the fastidious phloem-restricted gram-negative bacterium, vectored by the asian citrus psyllid. To date, there is no effective control available against CLas. To alleviate the effects of HLB on the industry and protect citrus farmers, there is an urgent need to identify or develop inhibitor molecules to suppress or eradicate CLas from infected citrus plant. In this paper, we demonstrate for the first time an in planta efficacy of two antimicrobial compounds against CLas viz. 2S albumin (a plant based protein; ~12.5 kDa), Nano-Zinc Oxide (Nano-ZnO; ~ 4.0 nm diameter) and their combinations. Aqueous formulations of these compounds were trunk-injected to HLB affected Mosambi plants (Citrus sinensis) grafted on 3-year old rough lemon (C. jambhiri) rootstock with known CLas titer maintained inside an insect-free screen house. The effective concentration of 2S albumin (330 ppm) coupled with the Nano-ZnO (330 ppm) at 1:1 ratio was used. The dynamics of CLas pathogen load of treated Mosambi plants was assessed using TaqMan-qPCR assay every 30 days after treatment (DAT) and monitored till 120 days. We observed that 2S albumin-Nano-ZnO formulation performed the best among all the treatments decreasing CLas population by 96.2%, 97.6%, 95.6%, and 97% of the initial bacterial load (per 12.5 ng of genomic DNA) at 30, 60, 90, and 120 DAT, respectively. Our studies demonstrated the potency of 2S albumin-Nano-ZnO formulation as an antimicrobial treatment for suppressing CLas in planta and could potentially be developed as a novel anti CLas therapeutics to mitigate the HLB severity affecting the citrus industry worldwide.
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spelling pubmed-61792202018-10-19 Antimicrobial nano-zinc oxide-2S albumin protein formulation significantly inhibits growth of “Candidatus Liberibacter asiaticus” in planta Ghosh, Dilip Kumar Kokane, Sunil Kumar, Pranav Ozcan, Ali Warghane, Ashish Motghare, Manali Santra, Swadeshmukul Sharma, Ashwani Kumar PLoS One Research Article Huanglongbing (HLB, also known as citrus greening) is considered to be the most devastating disease that has significantly damaged the citrus industry globally. HLB is caused by the Candidatus Liberibacter asiaticus (CLas), the fastidious phloem-restricted gram-negative bacterium, vectored by the asian citrus psyllid. To date, there is no effective control available against CLas. To alleviate the effects of HLB on the industry and protect citrus farmers, there is an urgent need to identify or develop inhibitor molecules to suppress or eradicate CLas from infected citrus plant. In this paper, we demonstrate for the first time an in planta efficacy of two antimicrobial compounds against CLas viz. 2S albumin (a plant based protein; ~12.5 kDa), Nano-Zinc Oxide (Nano-ZnO; ~ 4.0 nm diameter) and their combinations. Aqueous formulations of these compounds were trunk-injected to HLB affected Mosambi plants (Citrus sinensis) grafted on 3-year old rough lemon (C. jambhiri) rootstock with known CLas titer maintained inside an insect-free screen house. The effective concentration of 2S albumin (330 ppm) coupled with the Nano-ZnO (330 ppm) at 1:1 ratio was used. The dynamics of CLas pathogen load of treated Mosambi plants was assessed using TaqMan-qPCR assay every 30 days after treatment (DAT) and monitored till 120 days. We observed that 2S albumin-Nano-ZnO formulation performed the best among all the treatments decreasing CLas population by 96.2%, 97.6%, 95.6%, and 97% of the initial bacterial load (per 12.5 ng of genomic DNA) at 30, 60, 90, and 120 DAT, respectively. Our studies demonstrated the potency of 2S albumin-Nano-ZnO formulation as an antimicrobial treatment for suppressing CLas in planta and could potentially be developed as a novel anti CLas therapeutics to mitigate the HLB severity affecting the citrus industry worldwide. Public Library of Science 2018-10-10 /pmc/articles/PMC6179220/ /pubmed/30304000 http://dx.doi.org/10.1371/journal.pone.0204702 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Ghosh, Dilip Kumar
Kokane, Sunil
Kumar, Pranav
Ozcan, Ali
Warghane, Ashish
Motghare, Manali
Santra, Swadeshmukul
Sharma, Ashwani Kumar
Antimicrobial nano-zinc oxide-2S albumin protein formulation significantly inhibits growth of “Candidatus Liberibacter asiaticus” in planta
title Antimicrobial nano-zinc oxide-2S albumin protein formulation significantly inhibits growth of “Candidatus Liberibacter asiaticus” in planta
title_full Antimicrobial nano-zinc oxide-2S albumin protein formulation significantly inhibits growth of “Candidatus Liberibacter asiaticus” in planta
title_fullStr Antimicrobial nano-zinc oxide-2S albumin protein formulation significantly inhibits growth of “Candidatus Liberibacter asiaticus” in planta
title_full_unstemmed Antimicrobial nano-zinc oxide-2S albumin protein formulation significantly inhibits growth of “Candidatus Liberibacter asiaticus” in planta
title_short Antimicrobial nano-zinc oxide-2S albumin protein formulation significantly inhibits growth of “Candidatus Liberibacter asiaticus” in planta
title_sort antimicrobial nano-zinc oxide-2s albumin protein formulation significantly inhibits growth of “candidatus liberibacter asiaticus” in planta
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6179220/
https://www.ncbi.nlm.nih.gov/pubmed/30304000
http://dx.doi.org/10.1371/journal.pone.0204702
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