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In vitro evaluation of the antitumor effect of bismuth lipophilic nanoparticles (BisBAL NPs) on breast cancer cells
AIM: The objective of this study was to evaluate the antitumor activity of lipophilic bismuth nanoparticles (BisBAL NPs) on breast cancer cells. MATERIALS AND METHODS: The effect of varying concentrations of BisBAL NPs was evaluated on human MCF-7 breast cancer cells and on MCF-10A fibrocystic mamma...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6179729/ https://www.ncbi.nlm.nih.gov/pubmed/30323596 http://dx.doi.org/10.2147/IJN.S179095 |
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author | Hernandez-Delgadillo, Rene García-Cuéllar, Claudia María Sánchez-Pérez, Yesennia Pineda-Aguilar, Nayely Martínez-Martínez, Marco Antonio Rangel-Padilla, Eyra Elvyra Nakagoshi-Cepeda, Sergio Eduardo Solís-Soto, Juan Manuel Sánchez-Nájera, Rosa Isela Nakagoshi-Cepeda, María Argelia Akemi Chellam, Shankararaman Cabral-Romero, Claudio |
author_facet | Hernandez-Delgadillo, Rene García-Cuéllar, Claudia María Sánchez-Pérez, Yesennia Pineda-Aguilar, Nayely Martínez-Martínez, Marco Antonio Rangel-Padilla, Eyra Elvyra Nakagoshi-Cepeda, Sergio Eduardo Solís-Soto, Juan Manuel Sánchez-Nájera, Rosa Isela Nakagoshi-Cepeda, María Argelia Akemi Chellam, Shankararaman Cabral-Romero, Claudio |
author_sort | Hernandez-Delgadillo, Rene |
collection | PubMed |
description | AIM: The objective of this study was to evaluate the antitumor activity of lipophilic bismuth nanoparticles (BisBAL NPs) on breast cancer cells. MATERIALS AND METHODS: The effect of varying concentrations of BisBAL NPs was evaluated on human MCF-7 breast cancer cells and on MCF-10A fibrocystic mammary epitheliocytes as noncancer control cells. Cell viability was evaluated with the MTT assay, plasma membrane integrity was analyzed with the calcein AM assay, genotoxicity with the comet assay, and apoptosis with the Annexin V/7-AAD assay. RESULTS: BisBAL NPs were spherical in shape (average diameter, 28 nm) and agglomerated into dense electronic clusters. BisBAL NP induced a dose-dependent growth inhibition. Most importantly, growth inhibition was higher for MCF-7 cells than for MCF-10A cells. At 1 µM BisBAL NP, MCF-7 growth inhibition was 51%, while it was 11% for MCF-10A; at 25 µM BisBAL NP, the growth inhibition was 81% for MCF-7 and 24% for MCF-10A. With respect to mechanisms of action, a 24-hour exposure of 10 and 100 µM BisBAL NP caused loss of cell membrane integrity and fragmentation of tumor cell DNA. BisBAL NPs at 10 µM were genotoxic to and caused apoptosis of breast cancer cells. CONCLUSION: BisBAL NP-induced growth inhibition is dose dependent, and breast cancer cells are more vulnerable than noncancer breast cells. The mechanism of action of BisBAL NPs may include loss of plasma membrane integrity and a genotoxic effect on the genomic DNA of breast cancer cells. |
format | Online Article Text |
id | pubmed-6179729 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-61797292018-10-15 In vitro evaluation of the antitumor effect of bismuth lipophilic nanoparticles (BisBAL NPs) on breast cancer cells Hernandez-Delgadillo, Rene García-Cuéllar, Claudia María Sánchez-Pérez, Yesennia Pineda-Aguilar, Nayely Martínez-Martínez, Marco Antonio Rangel-Padilla, Eyra Elvyra Nakagoshi-Cepeda, Sergio Eduardo Solís-Soto, Juan Manuel Sánchez-Nájera, Rosa Isela Nakagoshi-Cepeda, María Argelia Akemi Chellam, Shankararaman Cabral-Romero, Claudio Int J Nanomedicine Original Research AIM: The objective of this study was to evaluate the antitumor activity of lipophilic bismuth nanoparticles (BisBAL NPs) on breast cancer cells. MATERIALS AND METHODS: The effect of varying concentrations of BisBAL NPs was evaluated on human MCF-7 breast cancer cells and on MCF-10A fibrocystic mammary epitheliocytes as noncancer control cells. Cell viability was evaluated with the MTT assay, plasma membrane integrity was analyzed with the calcein AM assay, genotoxicity with the comet assay, and apoptosis with the Annexin V/7-AAD assay. RESULTS: BisBAL NPs were spherical in shape (average diameter, 28 nm) and agglomerated into dense electronic clusters. BisBAL NP induced a dose-dependent growth inhibition. Most importantly, growth inhibition was higher for MCF-7 cells than for MCF-10A cells. At 1 µM BisBAL NP, MCF-7 growth inhibition was 51%, while it was 11% for MCF-10A; at 25 µM BisBAL NP, the growth inhibition was 81% for MCF-7 and 24% for MCF-10A. With respect to mechanisms of action, a 24-hour exposure of 10 and 100 µM BisBAL NP caused loss of cell membrane integrity and fragmentation of tumor cell DNA. BisBAL NPs at 10 µM were genotoxic to and caused apoptosis of breast cancer cells. CONCLUSION: BisBAL NP-induced growth inhibition is dose dependent, and breast cancer cells are more vulnerable than noncancer breast cells. The mechanism of action of BisBAL NPs may include loss of plasma membrane integrity and a genotoxic effect on the genomic DNA of breast cancer cells. Dove Medical Press 2018-10-05 /pmc/articles/PMC6179729/ /pubmed/30323596 http://dx.doi.org/10.2147/IJN.S179095 Text en © 2018 Hernandez-Delgadillo et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Hernandez-Delgadillo, Rene García-Cuéllar, Claudia María Sánchez-Pérez, Yesennia Pineda-Aguilar, Nayely Martínez-Martínez, Marco Antonio Rangel-Padilla, Eyra Elvyra Nakagoshi-Cepeda, Sergio Eduardo Solís-Soto, Juan Manuel Sánchez-Nájera, Rosa Isela Nakagoshi-Cepeda, María Argelia Akemi Chellam, Shankararaman Cabral-Romero, Claudio In vitro evaluation of the antitumor effect of bismuth lipophilic nanoparticles (BisBAL NPs) on breast cancer cells |
title | In vitro evaluation of the antitumor effect of bismuth lipophilic nanoparticles (BisBAL NPs) on breast cancer cells |
title_full | In vitro evaluation of the antitumor effect of bismuth lipophilic nanoparticles (BisBAL NPs) on breast cancer cells |
title_fullStr | In vitro evaluation of the antitumor effect of bismuth lipophilic nanoparticles (BisBAL NPs) on breast cancer cells |
title_full_unstemmed | In vitro evaluation of the antitumor effect of bismuth lipophilic nanoparticles (BisBAL NPs) on breast cancer cells |
title_short | In vitro evaluation of the antitumor effect of bismuth lipophilic nanoparticles (BisBAL NPs) on breast cancer cells |
title_sort | in vitro evaluation of the antitumor effect of bismuth lipophilic nanoparticles (bisbal nps) on breast cancer cells |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6179729/ https://www.ncbi.nlm.nih.gov/pubmed/30323596 http://dx.doi.org/10.2147/IJN.S179095 |
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