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Zika Virus Infection at Different Pregnancy Stages: Anatomopathological Findings, Target Cells and Viral Persistence in Placental Tissues

Zika virus (ZIKV) infection in humans has been associated with congenital malformations and other neurological disorders, such as Guillain-Barré syndrome. The mechanism(s) of ZIKV intrauterine transmission, the cell types involved, the most vulnerable period of pregnancy for severe outcomes from inf...

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Autores principales: de Noronha, Lucia, Zanluca, Camila, Burger, Marion, Suzukawa, Andreia Akemi, Azevedo, Marina, Rebutini, Patricia Z., Novadzki, Iolanda Maria, Tanabe, Laurina Setsuko, Presibella, Mayra Marinho, Duarte dos Santos, Claudia Nunes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6180237/
https://www.ncbi.nlm.nih.gov/pubmed/30337910
http://dx.doi.org/10.3389/fmicb.2018.02266
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author de Noronha, Lucia
Zanluca, Camila
Burger, Marion
Suzukawa, Andreia Akemi
Azevedo, Marina
Rebutini, Patricia Z.
Novadzki, Iolanda Maria
Tanabe, Laurina Setsuko
Presibella, Mayra Marinho
Duarte dos Santos, Claudia Nunes
author_facet de Noronha, Lucia
Zanluca, Camila
Burger, Marion
Suzukawa, Andreia Akemi
Azevedo, Marina
Rebutini, Patricia Z.
Novadzki, Iolanda Maria
Tanabe, Laurina Setsuko
Presibella, Mayra Marinho
Duarte dos Santos, Claudia Nunes
author_sort de Noronha, Lucia
collection PubMed
description Zika virus (ZIKV) infection in humans has been associated with congenital malformations and other neurological disorders, such as Guillain-Barré syndrome. The mechanism(s) of ZIKV intrauterine transmission, the cell types involved, the most vulnerable period of pregnancy for severe outcomes from infection and other physiopathological aspects are not completely elucidated. In this study, we analyzed placental samples obtained at the time of delivery from a group of 24 women diagnosed with ZIKV infection during the first, second or third trimesters of pregnancy. Villous immaturity was the main histological finding in the placental tissues, although placentas without alterations were also frequently observed. Significant enhancement of the number of syncytial sprouts was observed in the placentas of women infected during the third trimester, indicating the development of placental abnormalities after ZIKV infection. Hyperplasia of Hofbauer cells (HCs) was also observed in these third-trimester placental tissues, and remarkably, HCs were the only ZIKV-positive fetal cells found in the placentas studied that persisted until birth, as revealed by immunohistochemical (IHC) analysis. Thirty-three percent of women infected during pregnancy delivered infants with congenital abnormalities, although no pattern correlating the gestational stage at infection, the IHC positivity of HCs in placental tissues and the presence of congenital malformations at birth was observed. Placental tissue analysis enabled us to confirm maternal ZIKV infection in cases where serum from the acute infection phase was not available, which reinforces the importance of this technique in identifying possible causal factors of birth defects. The results we observed in the samples from naturally infected pregnant women may contribute to the understanding of some aspects of the pathophysiology of ZIKV.
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spelling pubmed-61802372018-10-18 Zika Virus Infection at Different Pregnancy Stages: Anatomopathological Findings, Target Cells and Viral Persistence in Placental Tissues de Noronha, Lucia Zanluca, Camila Burger, Marion Suzukawa, Andreia Akemi Azevedo, Marina Rebutini, Patricia Z. Novadzki, Iolanda Maria Tanabe, Laurina Setsuko Presibella, Mayra Marinho Duarte dos Santos, Claudia Nunes Front Microbiol Microbiology Zika virus (ZIKV) infection in humans has been associated with congenital malformations and other neurological disorders, such as Guillain-Barré syndrome. The mechanism(s) of ZIKV intrauterine transmission, the cell types involved, the most vulnerable period of pregnancy for severe outcomes from infection and other physiopathological aspects are not completely elucidated. In this study, we analyzed placental samples obtained at the time of delivery from a group of 24 women diagnosed with ZIKV infection during the first, second or third trimesters of pregnancy. Villous immaturity was the main histological finding in the placental tissues, although placentas without alterations were also frequently observed. Significant enhancement of the number of syncytial sprouts was observed in the placentas of women infected during the third trimester, indicating the development of placental abnormalities after ZIKV infection. Hyperplasia of Hofbauer cells (HCs) was also observed in these third-trimester placental tissues, and remarkably, HCs were the only ZIKV-positive fetal cells found in the placentas studied that persisted until birth, as revealed by immunohistochemical (IHC) analysis. Thirty-three percent of women infected during pregnancy delivered infants with congenital abnormalities, although no pattern correlating the gestational stage at infection, the IHC positivity of HCs in placental tissues and the presence of congenital malformations at birth was observed. Placental tissue analysis enabled us to confirm maternal ZIKV infection in cases where serum from the acute infection phase was not available, which reinforces the importance of this technique in identifying possible causal factors of birth defects. The results we observed in the samples from naturally infected pregnant women may contribute to the understanding of some aspects of the pathophysiology of ZIKV. Frontiers Media S.A. 2018-09-25 /pmc/articles/PMC6180237/ /pubmed/30337910 http://dx.doi.org/10.3389/fmicb.2018.02266 Text en Copyright © 2018 Noronha, Zanluca, Burger, Suzukawa, Azevedo, Rebutini, Novadzki, Tanabe, Presibella and Duarte dos Santos. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
de Noronha, Lucia
Zanluca, Camila
Burger, Marion
Suzukawa, Andreia Akemi
Azevedo, Marina
Rebutini, Patricia Z.
Novadzki, Iolanda Maria
Tanabe, Laurina Setsuko
Presibella, Mayra Marinho
Duarte dos Santos, Claudia Nunes
Zika Virus Infection at Different Pregnancy Stages: Anatomopathological Findings, Target Cells and Viral Persistence in Placental Tissues
title Zika Virus Infection at Different Pregnancy Stages: Anatomopathological Findings, Target Cells and Viral Persistence in Placental Tissues
title_full Zika Virus Infection at Different Pregnancy Stages: Anatomopathological Findings, Target Cells and Viral Persistence in Placental Tissues
title_fullStr Zika Virus Infection at Different Pregnancy Stages: Anatomopathological Findings, Target Cells and Viral Persistence in Placental Tissues
title_full_unstemmed Zika Virus Infection at Different Pregnancy Stages: Anatomopathological Findings, Target Cells and Viral Persistence in Placental Tissues
title_short Zika Virus Infection at Different Pregnancy Stages: Anatomopathological Findings, Target Cells and Viral Persistence in Placental Tissues
title_sort zika virus infection at different pregnancy stages: anatomopathological findings, target cells and viral persistence in placental tissues
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6180237/
https://www.ncbi.nlm.nih.gov/pubmed/30337910
http://dx.doi.org/10.3389/fmicb.2018.02266
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