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Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice

Foot-and-mouth disease (FMD) remains one of the most feared viral diseases affecting cloven-hoofed animals, and results in severe economic losses. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of recombinant FMDV-like particles (VLPs) as subunit vaccines has gained...

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Autores principales: Ruiz, Vanesa, Baztarrica, Josefina, Rybicki, Edward P., Meyers, Ann E., Wigdorovitz, Andrés
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6180338/
https://www.ncbi.nlm.nih.gov/pubmed/30319941
http://dx.doi.org/10.1016/j.btre.2018.e00283
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author Ruiz, Vanesa
Baztarrica, Josefina
Rybicki, Edward P.
Meyers, Ann E.
Wigdorovitz, Andrés
author_facet Ruiz, Vanesa
Baztarrica, Josefina
Rybicki, Edward P.
Meyers, Ann E.
Wigdorovitz, Andrés
author_sort Ruiz, Vanesa
collection PubMed
description Foot-and-mouth disease (FMD) remains one of the most feared viral diseases affecting cloven-hoofed animals, and results in severe economic losses. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of recombinant FMDV-like particles (VLPs) as subunit vaccines has gained importance because of their immunogenic properties and safety. We evaluated the production of FMD VLPs, via Agrobacterium-mediated transient expression, and the immunogenicity of these structures in mice. Leaves were infiltrated with pEAQ-HT and pRIC 3.0 vectors encoding the capsid precursor P1-2A and the protease 3C. The recombinant protein yield was 3–4 mg/kg of fresh leaf tissue. Both groups of mice immunized with purified VLPs and mice immunized with the crude leaf extract elicited a specific humoral response with similar antibody titers. Thus, minimally processed plant material containing transiently expressed FMD VLPs could be a scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV.
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spelling pubmed-61803382018-10-12 Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice Ruiz, Vanesa Baztarrica, Josefina Rybicki, Edward P. Meyers, Ann E. Wigdorovitz, Andrés Biotechnol Rep (Amst) Article Foot-and-mouth disease (FMD) remains one of the most feared viral diseases affecting cloven-hoofed animals, and results in severe economic losses. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of recombinant FMDV-like particles (VLPs) as subunit vaccines has gained importance because of their immunogenic properties and safety. We evaluated the production of FMD VLPs, via Agrobacterium-mediated transient expression, and the immunogenicity of these structures in mice. Leaves were infiltrated with pEAQ-HT and pRIC 3.0 vectors encoding the capsid precursor P1-2A and the protease 3C. The recombinant protein yield was 3–4 mg/kg of fresh leaf tissue. Both groups of mice immunized with purified VLPs and mice immunized with the crude leaf extract elicited a specific humoral response with similar antibody titers. Thus, minimally processed plant material containing transiently expressed FMD VLPs could be a scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV. Elsevier 2018-09-28 /pmc/articles/PMC6180338/ /pubmed/30319941 http://dx.doi.org/10.1016/j.btre.2018.e00283 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Ruiz, Vanesa
Baztarrica, Josefina
Rybicki, Edward P.
Meyers, Ann E.
Wigdorovitz, Andrés
Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice
title Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice
title_full Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice
title_fullStr Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice
title_full_unstemmed Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice
title_short Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice
title_sort minimally processed crude leaf extracts of nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6180338/
https://www.ncbi.nlm.nih.gov/pubmed/30319941
http://dx.doi.org/10.1016/j.btre.2018.e00283
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