Cargando…

Phenotypic and genotypic characterization of macrolide, lincosamide and streptogramin B resistance among clinical isolates of staphylococci in southwest of Iran

OBJECTIVE: The present study aimed to determine the phenotypic and genotypic profile of macrolide, lincosamide and streptogramin B (MLS(B)) resistance in clinical isolates of staphylococci. RESULTS: This cross-sectional study was conducted on 164 non-duplicated staphylococci isolates collected durin...

Descripción completa

Detalles Bibliográficos
Autores principales: Khashei, Reza, Malekzadegan, Yalda, Sedigh Ebrahim-Saraie, Hadi, Razavi, Zahra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6180372/
https://www.ncbi.nlm.nih.gov/pubmed/30305181
http://dx.doi.org/10.1186/s13104-018-3817-4
_version_ 1783362186975903744
author Khashei, Reza
Malekzadegan, Yalda
Sedigh Ebrahim-Saraie, Hadi
Razavi, Zahra
author_facet Khashei, Reza
Malekzadegan, Yalda
Sedigh Ebrahim-Saraie, Hadi
Razavi, Zahra
author_sort Khashei, Reza
collection PubMed
description OBJECTIVE: The present study aimed to determine the phenotypic and genotypic profile of macrolide, lincosamide and streptogramin B (MLS(B)) resistance in clinical isolates of staphylococci. RESULTS: This cross-sectional study was conducted on 164 non-duplicated staphylococci isolates collected during August 2015 to February 2016 from two tertiary care hospitals in Shiraz, southwest of Iran. Of the 164 isolates, 86 erythromycin-resistant isolates consist of 35 Staphylococcus aureus and 51 coagulase negative staphylococci (CoNS) were included in the study. Of the 35 S. aureus, the prevalence of cMLS (constitutive), iMLS (inducible), and MS phenotypes were found 82.9%, 8.6% and 8.6%, respectively. Among 51 CoNS, the frequencies of cMLS, iMLS, and MS phenotypes were detected 66.7%, 11.8% and 21.6%, respectively. Among S. aureus isolates, the predominant genes were ermC in 82.9% isolates, followed by ermA in 57.1% and msrA in 28.6% of isolates. Among CoNS isolates, the most frequent genes were diagnosed ermC in 70.6% isolates followed by msrA in 68.6% and ermA in 11.8% of isolates. In conclusion, regarding the presence of MLS(B) resistance in our region, diagnosis of this resistance type on a routine basis in staphylococcal clinical isolates is of particular importance.
format Online
Article
Text
id pubmed-6180372
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-61803722018-10-18 Phenotypic and genotypic characterization of macrolide, lincosamide and streptogramin B resistance among clinical isolates of staphylococci in southwest of Iran Khashei, Reza Malekzadegan, Yalda Sedigh Ebrahim-Saraie, Hadi Razavi, Zahra BMC Res Notes Research Note OBJECTIVE: The present study aimed to determine the phenotypic and genotypic profile of macrolide, lincosamide and streptogramin B (MLS(B)) resistance in clinical isolates of staphylococci. RESULTS: This cross-sectional study was conducted on 164 non-duplicated staphylococci isolates collected during August 2015 to February 2016 from two tertiary care hospitals in Shiraz, southwest of Iran. Of the 164 isolates, 86 erythromycin-resistant isolates consist of 35 Staphylococcus aureus and 51 coagulase negative staphylococci (CoNS) were included in the study. Of the 35 S. aureus, the prevalence of cMLS (constitutive), iMLS (inducible), and MS phenotypes were found 82.9%, 8.6% and 8.6%, respectively. Among 51 CoNS, the frequencies of cMLS, iMLS, and MS phenotypes were detected 66.7%, 11.8% and 21.6%, respectively. Among S. aureus isolates, the predominant genes were ermC in 82.9% isolates, followed by ermA in 57.1% and msrA in 28.6% of isolates. Among CoNS isolates, the most frequent genes were diagnosed ermC in 70.6% isolates followed by msrA in 68.6% and ermA in 11.8% of isolates. In conclusion, regarding the presence of MLS(B) resistance in our region, diagnosis of this resistance type on a routine basis in staphylococcal clinical isolates is of particular importance. BioMed Central 2018-10-10 /pmc/articles/PMC6180372/ /pubmed/30305181 http://dx.doi.org/10.1186/s13104-018-3817-4 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Khashei, Reza
Malekzadegan, Yalda
Sedigh Ebrahim-Saraie, Hadi
Razavi, Zahra
Phenotypic and genotypic characterization of macrolide, lincosamide and streptogramin B resistance among clinical isolates of staphylococci in southwest of Iran
title Phenotypic and genotypic characterization of macrolide, lincosamide and streptogramin B resistance among clinical isolates of staphylococci in southwest of Iran
title_full Phenotypic and genotypic characterization of macrolide, lincosamide and streptogramin B resistance among clinical isolates of staphylococci in southwest of Iran
title_fullStr Phenotypic and genotypic characterization of macrolide, lincosamide and streptogramin B resistance among clinical isolates of staphylococci in southwest of Iran
title_full_unstemmed Phenotypic and genotypic characterization of macrolide, lincosamide and streptogramin B resistance among clinical isolates of staphylococci in southwest of Iran
title_short Phenotypic and genotypic characterization of macrolide, lincosamide and streptogramin B resistance among clinical isolates of staphylococci in southwest of Iran
title_sort phenotypic and genotypic characterization of macrolide, lincosamide and streptogramin b resistance among clinical isolates of staphylococci in southwest of iran
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6180372/
https://www.ncbi.nlm.nih.gov/pubmed/30305181
http://dx.doi.org/10.1186/s13104-018-3817-4
work_keys_str_mv AT khasheireza phenotypicandgenotypiccharacterizationofmacrolidelincosamideandstreptograminbresistanceamongclinicalisolatesofstaphylococciinsouthwestofiran
AT malekzadeganyalda phenotypicandgenotypiccharacterizationofmacrolidelincosamideandstreptograminbresistanceamongclinicalisolatesofstaphylococciinsouthwestofiran
AT sedighebrahimsaraiehadi phenotypicandgenotypiccharacterizationofmacrolidelincosamideandstreptograminbresistanceamongclinicalisolatesofstaphylococciinsouthwestofiran
AT razavizahra phenotypicandgenotypiccharacterizationofmacrolidelincosamideandstreptograminbresistanceamongclinicalisolatesofstaphylococciinsouthwestofiran