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Blood cytokine expression correlates with early multi-organ damage in a mouse model of moderate hypothermia with circulatory arrest using cardiopulmonary bypass

Cardiopulmonary bypass (CPB) with moderate hypothermic cardiac arrest (MHCA) is essential for prolonged complex procedures in cardiac surgery and is associated with postoperative complications. Although cytokine release provoked through MHCA under CPB plays a pivotal role in postoperative organ dama...

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Autores principales: Natanov, Ruslan, Gueler, Faikah, Falk, Christine S., Kühn, Christian, Maus, Ulrich, Boyle, Erin C., Siemeni, Thierry, Knoefel, Ann-Katrin, Cebotari, Serghei, Haverich, Axel, Madrahimov, Nodir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6181365/
https://www.ncbi.nlm.nih.gov/pubmed/30308065
http://dx.doi.org/10.1371/journal.pone.0205437
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author Natanov, Ruslan
Gueler, Faikah
Falk, Christine S.
Kühn, Christian
Maus, Ulrich
Boyle, Erin C.
Siemeni, Thierry
Knoefel, Ann-Katrin
Cebotari, Serghei
Haverich, Axel
Madrahimov, Nodir
author_facet Natanov, Ruslan
Gueler, Faikah
Falk, Christine S.
Kühn, Christian
Maus, Ulrich
Boyle, Erin C.
Siemeni, Thierry
Knoefel, Ann-Katrin
Cebotari, Serghei
Haverich, Axel
Madrahimov, Nodir
author_sort Natanov, Ruslan
collection PubMed
description Cardiopulmonary bypass (CPB) with moderate hypothermic cardiac arrest (MHCA) is essential for prolonged complex procedures in cardiac surgery and is associated with postoperative complications. Although cytokine release provoked through MHCA under CPB plays a pivotal role in postoperative organ damage, the pathomechanisms are unclear. Here, we investigated the cytokine release pattern and histological organ damage after MHCA using a recently described mouse CPB model. Eight BALB/c mice underwent 60 minutes of circulatory arrest under CPB, were successively rewarmed and reperfused. Blood cytokine concentrations and liver and kidney function parameters were measured and histological changes to these organs were compared to control animals. Our results showed a marked increase in proinflammatory cytokines and histological changes in the kidney, lung, and liver after CPB. Furthermore, clinical chemistry showed signs of hemolysis and acute kidney injury. These results suggest early onset of solid organ injury which correlates with increased leukocyte infiltration. A better understanding of the interplay between pro-inflammatory cytokine activation and solid organ injury in this model of CBP with MHCA will inform strategies to reduce organ damage during cardiac surgeries in the clinic.
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spelling pubmed-61813652018-10-26 Blood cytokine expression correlates with early multi-organ damage in a mouse model of moderate hypothermia with circulatory arrest using cardiopulmonary bypass Natanov, Ruslan Gueler, Faikah Falk, Christine S. Kühn, Christian Maus, Ulrich Boyle, Erin C. Siemeni, Thierry Knoefel, Ann-Katrin Cebotari, Serghei Haverich, Axel Madrahimov, Nodir PLoS One Research Article Cardiopulmonary bypass (CPB) with moderate hypothermic cardiac arrest (MHCA) is essential for prolonged complex procedures in cardiac surgery and is associated with postoperative complications. Although cytokine release provoked through MHCA under CPB plays a pivotal role in postoperative organ damage, the pathomechanisms are unclear. Here, we investigated the cytokine release pattern and histological organ damage after MHCA using a recently described mouse CPB model. Eight BALB/c mice underwent 60 minutes of circulatory arrest under CPB, were successively rewarmed and reperfused. Blood cytokine concentrations and liver and kidney function parameters were measured and histological changes to these organs were compared to control animals. Our results showed a marked increase in proinflammatory cytokines and histological changes in the kidney, lung, and liver after CPB. Furthermore, clinical chemistry showed signs of hemolysis and acute kidney injury. These results suggest early onset of solid organ injury which correlates with increased leukocyte infiltration. A better understanding of the interplay between pro-inflammatory cytokine activation and solid organ injury in this model of CBP with MHCA will inform strategies to reduce organ damage during cardiac surgeries in the clinic. Public Library of Science 2018-10-11 /pmc/articles/PMC6181365/ /pubmed/30308065 http://dx.doi.org/10.1371/journal.pone.0205437 Text en © 2018 Natanov et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Natanov, Ruslan
Gueler, Faikah
Falk, Christine S.
Kühn, Christian
Maus, Ulrich
Boyle, Erin C.
Siemeni, Thierry
Knoefel, Ann-Katrin
Cebotari, Serghei
Haverich, Axel
Madrahimov, Nodir
Blood cytokine expression correlates with early multi-organ damage in a mouse model of moderate hypothermia with circulatory arrest using cardiopulmonary bypass
title Blood cytokine expression correlates with early multi-organ damage in a mouse model of moderate hypothermia with circulatory arrest using cardiopulmonary bypass
title_full Blood cytokine expression correlates with early multi-organ damage in a mouse model of moderate hypothermia with circulatory arrest using cardiopulmonary bypass
title_fullStr Blood cytokine expression correlates with early multi-organ damage in a mouse model of moderate hypothermia with circulatory arrest using cardiopulmonary bypass
title_full_unstemmed Blood cytokine expression correlates with early multi-organ damage in a mouse model of moderate hypothermia with circulatory arrest using cardiopulmonary bypass
title_short Blood cytokine expression correlates with early multi-organ damage in a mouse model of moderate hypothermia with circulatory arrest using cardiopulmonary bypass
title_sort blood cytokine expression correlates with early multi-organ damage in a mouse model of moderate hypothermia with circulatory arrest using cardiopulmonary bypass
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6181365/
https://www.ncbi.nlm.nih.gov/pubmed/30308065
http://dx.doi.org/10.1371/journal.pone.0205437
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