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A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport
Transport of signaling molecules is of major importance for regulating plant growth, development, and responses to the environment. A prime example is the spatial-distribution of auxin, which is regulated via transporters to govern developmental patterning. A critical limitation in our ability to id...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182007/ https://www.ncbi.nlm.nih.gov/pubmed/30310073 http://dx.doi.org/10.1038/s41467-018-06410-y |
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author | Zhang, Yuqin Nasser, Victoria Pisanty, Odelia Omary, Moutasem Wulff, Nikolai Di Donato, Martin Tal, Iris Hauser, Felix Hao, Pengchao Roth, Ohad Fromm, Hillel Schroeder, Julian I. Geisler, Markus Nour-Eldin, Hussam Hassan Shani, Eilon |
author_facet | Zhang, Yuqin Nasser, Victoria Pisanty, Odelia Omary, Moutasem Wulff, Nikolai Di Donato, Martin Tal, Iris Hauser, Felix Hao, Pengchao Roth, Ohad Fromm, Hillel Schroeder, Julian I. Geisler, Markus Nour-Eldin, Hussam Hassan Shani, Eilon |
author_sort | Zhang, Yuqin |
collection | PubMed |
description | Transport of signaling molecules is of major importance for regulating plant growth, development, and responses to the environment. A prime example is the spatial-distribution of auxin, which is regulated via transporters to govern developmental patterning. A critical limitation in our ability to identify transporters by forward genetic screens is their potential functional redundancy. Here, we overcome part of this functional redundancy via a transportome, multi-targeted forward-genetic screen using artificial-microRNAs (amiRNAs). We generate a library of 3000 plant lines expressing 1777 amiRNAs, designed to target closely homologous genes within subclades of transporter families and identify, genotype and quantitatively phenotype, 80 lines showing reproducible shoot growth phenotypes. Within this population, we discover and characterize a strong redundant role for the unstudied ABCB6 and ABCB20 genes in auxin transport and response. The unique multi-targeted lines generated in this study could serve as a genetic resource that is expected to reveal additional transporters. |
format | Online Article Text |
id | pubmed-6182007 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-61820072018-10-15 A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport Zhang, Yuqin Nasser, Victoria Pisanty, Odelia Omary, Moutasem Wulff, Nikolai Di Donato, Martin Tal, Iris Hauser, Felix Hao, Pengchao Roth, Ohad Fromm, Hillel Schroeder, Julian I. Geisler, Markus Nour-Eldin, Hussam Hassan Shani, Eilon Nat Commun Article Transport of signaling molecules is of major importance for regulating plant growth, development, and responses to the environment. A prime example is the spatial-distribution of auxin, which is regulated via transporters to govern developmental patterning. A critical limitation in our ability to identify transporters by forward genetic screens is their potential functional redundancy. Here, we overcome part of this functional redundancy via a transportome, multi-targeted forward-genetic screen using artificial-microRNAs (amiRNAs). We generate a library of 3000 plant lines expressing 1777 amiRNAs, designed to target closely homologous genes within subclades of transporter families and identify, genotype and quantitatively phenotype, 80 lines showing reproducible shoot growth phenotypes. Within this population, we discover and characterize a strong redundant role for the unstudied ABCB6 and ABCB20 genes in auxin transport and response. The unique multi-targeted lines generated in this study could serve as a genetic resource that is expected to reveal additional transporters. Nature Publishing Group UK 2018-10-11 /pmc/articles/PMC6182007/ /pubmed/30310073 http://dx.doi.org/10.1038/s41467-018-06410-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Zhang, Yuqin Nasser, Victoria Pisanty, Odelia Omary, Moutasem Wulff, Nikolai Di Donato, Martin Tal, Iris Hauser, Felix Hao, Pengchao Roth, Ohad Fromm, Hillel Schroeder, Julian I. Geisler, Markus Nour-Eldin, Hussam Hassan Shani, Eilon A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport |
title | A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport |
title_full | A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport |
title_fullStr | A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport |
title_full_unstemmed | A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport |
title_short | A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport |
title_sort | transportome-scale amirna-based screen identifies redundant roles of arabidopsis abcb6 and abcb20 in auxin transport |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182007/ https://www.ncbi.nlm.nih.gov/pubmed/30310073 http://dx.doi.org/10.1038/s41467-018-06410-y |
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