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A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport

Transport of signaling molecules is of major importance for regulating plant growth, development, and responses to the environment. A prime example is the spatial-distribution of auxin, which is regulated via transporters to govern developmental patterning. A critical limitation in our ability to id...

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Autores principales: Zhang, Yuqin, Nasser, Victoria, Pisanty, Odelia, Omary, Moutasem, Wulff, Nikolai, Di Donato, Martin, Tal, Iris, Hauser, Felix, Hao, Pengchao, Roth, Ohad, Fromm, Hillel, Schroeder, Julian I., Geisler, Markus, Nour-Eldin, Hussam Hassan, Shani, Eilon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182007/
https://www.ncbi.nlm.nih.gov/pubmed/30310073
http://dx.doi.org/10.1038/s41467-018-06410-y
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author Zhang, Yuqin
Nasser, Victoria
Pisanty, Odelia
Omary, Moutasem
Wulff, Nikolai
Di Donato, Martin
Tal, Iris
Hauser, Felix
Hao, Pengchao
Roth, Ohad
Fromm, Hillel
Schroeder, Julian I.
Geisler, Markus
Nour-Eldin, Hussam Hassan
Shani, Eilon
author_facet Zhang, Yuqin
Nasser, Victoria
Pisanty, Odelia
Omary, Moutasem
Wulff, Nikolai
Di Donato, Martin
Tal, Iris
Hauser, Felix
Hao, Pengchao
Roth, Ohad
Fromm, Hillel
Schroeder, Julian I.
Geisler, Markus
Nour-Eldin, Hussam Hassan
Shani, Eilon
author_sort Zhang, Yuqin
collection PubMed
description Transport of signaling molecules is of major importance for regulating plant growth, development, and responses to the environment. A prime example is the spatial-distribution of auxin, which is regulated via transporters to govern developmental patterning. A critical limitation in our ability to identify transporters by forward genetic screens is their potential functional redundancy. Here, we overcome part of this functional redundancy via a transportome, multi-targeted forward-genetic screen using artificial-microRNAs (amiRNAs). We generate a library of 3000 plant lines expressing 1777 amiRNAs, designed to target closely homologous genes within subclades of transporter families and identify, genotype and quantitatively phenotype, 80 lines showing reproducible shoot growth phenotypes. Within this population, we discover and characterize a strong redundant role for the unstudied ABCB6 and ABCB20 genes in auxin transport and response. The unique multi-targeted lines generated in this study could serve as a genetic resource that is expected to reveal additional transporters.
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spelling pubmed-61820072018-10-15 A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport Zhang, Yuqin Nasser, Victoria Pisanty, Odelia Omary, Moutasem Wulff, Nikolai Di Donato, Martin Tal, Iris Hauser, Felix Hao, Pengchao Roth, Ohad Fromm, Hillel Schroeder, Julian I. Geisler, Markus Nour-Eldin, Hussam Hassan Shani, Eilon Nat Commun Article Transport of signaling molecules is of major importance for regulating plant growth, development, and responses to the environment. A prime example is the spatial-distribution of auxin, which is regulated via transporters to govern developmental patterning. A critical limitation in our ability to identify transporters by forward genetic screens is their potential functional redundancy. Here, we overcome part of this functional redundancy via a transportome, multi-targeted forward-genetic screen using artificial-microRNAs (amiRNAs). We generate a library of 3000 plant lines expressing 1777 amiRNAs, designed to target closely homologous genes within subclades of transporter families and identify, genotype and quantitatively phenotype, 80 lines showing reproducible shoot growth phenotypes. Within this population, we discover and characterize a strong redundant role for the unstudied ABCB6 and ABCB20 genes in auxin transport and response. The unique multi-targeted lines generated in this study could serve as a genetic resource that is expected to reveal additional transporters. Nature Publishing Group UK 2018-10-11 /pmc/articles/PMC6182007/ /pubmed/30310073 http://dx.doi.org/10.1038/s41467-018-06410-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhang, Yuqin
Nasser, Victoria
Pisanty, Odelia
Omary, Moutasem
Wulff, Nikolai
Di Donato, Martin
Tal, Iris
Hauser, Felix
Hao, Pengchao
Roth, Ohad
Fromm, Hillel
Schroeder, Julian I.
Geisler, Markus
Nour-Eldin, Hussam Hassan
Shani, Eilon
A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport
title A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport
title_full A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport
title_fullStr A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport
title_full_unstemmed A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport
title_short A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport
title_sort transportome-scale amirna-based screen identifies redundant roles of arabidopsis abcb6 and abcb20 in auxin transport
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182007/
https://www.ncbi.nlm.nih.gov/pubmed/30310073
http://dx.doi.org/10.1038/s41467-018-06410-y
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