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Bactericidal Property of Oregano Oil Against Multidrug-Resistant Clinical Isolates

Development of non-antibiotic alternatives to treat infections caused by multidrug-resistant (MDR) microbes represents one of the top priorities in healthcare and community settings, especially in the care of combat trauma-associated wound infections. Here, we investigate efficacy of oregano oil aga...

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Detalles Bibliográficos
Autores principales: Lu, Min, Dai, Tianhong, Murray, Clinton K., Wu, Mei X.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182053/
https://www.ncbi.nlm.nih.gov/pubmed/30344513
http://dx.doi.org/10.3389/fmicb.2018.02329
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author Lu, Min
Dai, Tianhong
Murray, Clinton K.
Wu, Mei X.
author_facet Lu, Min
Dai, Tianhong
Murray, Clinton K.
Wu, Mei X.
author_sort Lu, Min
collection PubMed
description Development of non-antibiotic alternatives to treat infections caused by multidrug-resistant (MDR) microbes represents one of the top priorities in healthcare and community settings, especially in the care of combat trauma-associated wound infections. Here, we investigate efficacy of oregano oil against pathogenic bacteria including MDR isolates from the combat casualties in vitro and in a mouse burn model. Oregano oil showed a significant anti-bacterial activity against 11 MDR clinical isolates including four Acinetobacter baumannii, three Pseudomonas aeruginosa, and four methicillin-resistant Staphylococcus aureus (MRSA) obtained from combat casualties and two luminescent strains of PA01 and MRSA USA300, with a MIC ranging from 0.08 mg/ml to 0.64 mg/ml. Oregano oil also effectively eradicated biofilms formed by each of the 13 pathogens above at similar MICs. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) revealed that oregano oil damaged bacterial cells and altered the morphology of their biofilms. While efficiently inactivating bacteria, there was no evidence of resistance development after up to 20 consecutive passages of representative bacterial strains in the presence of sublethal doses of oregano oil. In vivo study using the third-degree burn wounds infected with PA01 or USA300 demonstrated that oregano oil, topically applied 24 h after bacterial inoculation, sufficiently reduced the bacterial load in the wounds by 3 log(10) in 1 h, as measured by drastic reduction of bacterial bioluminescence. This bactericidal activity of oregano oil concurred with no significant side effect on the skin histologically or genotoxicity after three topical applications of oregano oil at 10 mg/ml for three consecutive days. The investigation suggests potentials of oregano oil as an alternative to antibiotics for the treatment of wound-associated infections regardless of antibiotic susceptibility.
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spelling pubmed-61820532018-10-19 Bactericidal Property of Oregano Oil Against Multidrug-Resistant Clinical Isolates Lu, Min Dai, Tianhong Murray, Clinton K. Wu, Mei X. Front Microbiol Microbiology Development of non-antibiotic alternatives to treat infections caused by multidrug-resistant (MDR) microbes represents one of the top priorities in healthcare and community settings, especially in the care of combat trauma-associated wound infections. Here, we investigate efficacy of oregano oil against pathogenic bacteria including MDR isolates from the combat casualties in vitro and in a mouse burn model. Oregano oil showed a significant anti-bacterial activity against 11 MDR clinical isolates including four Acinetobacter baumannii, three Pseudomonas aeruginosa, and four methicillin-resistant Staphylococcus aureus (MRSA) obtained from combat casualties and two luminescent strains of PA01 and MRSA USA300, with a MIC ranging from 0.08 mg/ml to 0.64 mg/ml. Oregano oil also effectively eradicated biofilms formed by each of the 13 pathogens above at similar MICs. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) revealed that oregano oil damaged bacterial cells and altered the morphology of their biofilms. While efficiently inactivating bacteria, there was no evidence of resistance development after up to 20 consecutive passages of representative bacterial strains in the presence of sublethal doses of oregano oil. In vivo study using the third-degree burn wounds infected with PA01 or USA300 demonstrated that oregano oil, topically applied 24 h after bacterial inoculation, sufficiently reduced the bacterial load in the wounds by 3 log(10) in 1 h, as measured by drastic reduction of bacterial bioluminescence. This bactericidal activity of oregano oil concurred with no significant side effect on the skin histologically or genotoxicity after three topical applications of oregano oil at 10 mg/ml for three consecutive days. The investigation suggests potentials of oregano oil as an alternative to antibiotics for the treatment of wound-associated infections regardless of antibiotic susceptibility. Frontiers Media S.A. 2018-10-05 /pmc/articles/PMC6182053/ /pubmed/30344513 http://dx.doi.org/10.3389/fmicb.2018.02329 Text en Copyright © 2018 Lu, Dai, Murray and Wu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Lu, Min
Dai, Tianhong
Murray, Clinton K.
Wu, Mei X.
Bactericidal Property of Oregano Oil Against Multidrug-Resistant Clinical Isolates
title Bactericidal Property of Oregano Oil Against Multidrug-Resistant Clinical Isolates
title_full Bactericidal Property of Oregano Oil Against Multidrug-Resistant Clinical Isolates
title_fullStr Bactericidal Property of Oregano Oil Against Multidrug-Resistant Clinical Isolates
title_full_unstemmed Bactericidal Property of Oregano Oil Against Multidrug-Resistant Clinical Isolates
title_short Bactericidal Property of Oregano Oil Against Multidrug-Resistant Clinical Isolates
title_sort bactericidal property of oregano oil against multidrug-resistant clinical isolates
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182053/
https://www.ncbi.nlm.nih.gov/pubmed/30344513
http://dx.doi.org/10.3389/fmicb.2018.02329
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