Cargando…

SRB-2: a promiscuous rainbow aptamer for live-cell RNA imaging

The SRB-2 aptamer originally selected against sulforhodamine B is shown here to promiscuously bind to various dyes with different colors. Binding of SRB-2 to these dyes results in either fluorescence increase or decrease, making them attractive for fluorescence microscopy and biological assays. By s...

Descripción completa

Detalles Bibliográficos
Autores principales:	Sunbul, Murat, Jäschke, Andres
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2018
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182184/ https://www.ncbi.nlm.nih.gov/pubmed/29931157 http://dx.doi.org/10.1093/nar/gky543

Ejemplares similares

Dual-colour imaging of RNAs using quencher- and fluorophore-binding aptamers
por: Arora, Ankita, et al.
Publicado: (2015)

A Color‐Shifting Near‐Infrared Fluorescent Aptamer–Fluorophore Module for Live‐Cell RNA Imaging
por: Zhang, Jingye, et al.
Publicado: (2021)

Inert Pepper aptamer-mediated endogenous mRNA recognition and imaging in living cells
por: Wang, Qi, et al.
Publicado: (2022)

In silico selection of RNA aptamers
por: Chushak, Yaroslav, et al.
Publicado: (2009)

An on-bead tailing/ligation approach for sequencing resin-bound RNA libraries
por: Wiesmayr, Anna, et al.
Publicado: (2012)

Aptamer mediated siRNA delivery
por: Chu, Ted C., et al.
Publicado: (2006)

Composite RNA aptamers as functional mimics of proteins
por: Xu, Daiying, et al.
Publicado: (2009)

Site-specific terminal and internal labeling of RNA by poly(A) polymerase tailing and copper-catalyzed or copper-free strain-promoted click chemistry
por: Winz, Marie-Luise, et al.
Publicado: (2012)

In vivo expression and purification of aptamer-tagged small RNA regulators
por: Said, Nelly, et al.
Publicado: (2009)

RNA aptamer capture of macromolecular complexes for mass spectrometry analysis
por: Ray, Judhajeet, et al.
Publicado: (2020)

RNA aptamers that functionally interact with green fluorescent protein and its derivatives
por: Shui, Bo, et al.
Publicado: (2012)

Knocking down gene function with an RNA aptamer expressed as part of an intron
por: Wang, Shengchun, et al.
Publicado: (2010)

Aptamer-guided gene targeting in yeast and human cells
por: Ruff, Patrick, et al.
Publicado: (2014)

An optimized streptavidin-binding RNA aptamer for purification of ribonucleoprotein complexes identifies novel ARE-binding proteins
por: Leppek, Kathrin, et al.
Publicado: (2014)

Selection of aptamers for a protein target in cell lysate and their application to protein purification
por: Javaherian, Sahar, et al.
Publicado: (2009)

Live-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters
por: Shin, Ilchung, et al.
Publicado: (2014)

Selection of DNA aptamers using atomic force microscopy
por: Miyachi, Yusuke, et al.
Publicado: (2010)

Fast-exchanging spirocyclic rhodamine probes for aptamer-based super-resolution RNA imaging
por: Englert, Daniel, et al.
Publicado: (2023)

Photocaged amplified FRET nanoflares: spatiotemporal controllable of mRNA-powered nanomachines for precise and sensitive microRNA imaging in live cells
por: Li, Jing, et al.
Publicado: (2021)

Development of an automated in vitro selection protocol to obtain RNA-based aptamers: identification of a biostable substance P antagonist
por: Eulberg, Dirk, et al.
Publicado: (2005)

Reversible site-specific tagging of enzymatically synthesized RNAs using aldehyde–hydrazine chemistry and protease-cleavable linkers
por: Pfander, Stephanie, et al.
Publicado: (2007)

Nucleotidyl transferase assisted DNA labeling with different click chemistries
por: Winz, Marie-Luise, et al.
Publicado: (2015)

Co-expression of anti-NFκB RNA aptamers and siRNAs leads to maximal suppression of NFκB activity in mammalian cells
por: Chan, Robert, et al.
Publicado: (2006)

Visualization and characterization of RNA–protein interactions in living cells
por: Duan, Ningjun, et al.
Publicado: (2021)

Proximity extension of circular DNA aptamers with real-time protein detection
por: Di Giusto, Daniel A., et al.
Publicado: (2005)

Commandeering a biological pathway using aptamer-derived molecular adaptors
por: Mallik, Prabhat K., et al.
Publicado: (2010)

High-throughput quantitative binding analysis of DNA aptamers using exonucleases
por: Canoura, Juan, et al.
Publicado: (2022)

Quantitative assessment of ratiometric bimolecular beacons as a tool for imaging single engineered RNA transcripts and measuring gene expression in living cells
por: Zhang, Xuemei, et al.
Publicado: (2013)

In vitro isolation of small-molecule-binding aptamers with intrinsic dye-displacement functionality
por: Yu, Haixiang, et al.
Publicado: (2018)

Engineering a ribozyme cleavage-induced split fluorescent aptamer complementation assay
por: Ausländer, Simon, et al.
Publicado: (2016)

Robust gene expression control in human cells with a novel universal TetR aptamer splicing module
por: Mol, Adam A, et al.
Publicado: (2019)

Real time monitoring of endogenous cytoplasmic mRNA using linear antisense 2′-O-methyl RNA probes in living cells
por: Okabe, Kohki, et al.
Publicado: (2011)

Ratiometric bimolecular beacons for the sensitive detection of RNA in single living cells
por: Chen, Antony K., et al.
Publicado: (2010)

ECHO-liveFISH: in vivo RNA labeling reveals dynamic regulation of nuclear RNA foci in living tissues
por: Oomoto, Ikumi, et al.
Publicado: (2015)

Array-based evolution of DNA aptamers allows modelling of an explicit sequence-fitness landscape
por: Knight, Christopher G., et al.
Publicado: (2009)

Light-up properties of complexes between thiazole orange-small molecule conjugates and aptamers
por: Pei, Renjun, et al.
Publicado: (2009)

Characterisation of aptamer–target interactions by branched selection and high-throughput sequencing of SELEX pools
por: Dupont, Daniel M., et al.
Publicado: (2015)

Label-free profiling of DNA aptamer-small molecule binding using T5 exonuclease
por: Alkhamis, Obtin, et al.
Publicado: (2020)

A CRISPR/molecular beacon hybrid system for live-cell genomic imaging
por: Wu, Xiaotian, et al.
Publicado: (2018)

Introducing structure-switching functionality into small-molecule-binding aptamers via nuclease-directed truncation
por: Wang, Zongwen, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_6kar6t8mv2aqgj41gqr0e1q8qu