Development and Characterization of a New Cell Line from Olive Flounder Paralichthys olivaceus

A new embryonic cell line (OFEC-17FEN) derived from olive flounder Paralichthys olivaceus was developed. OFEC-17FEN cells were subcultured for <30 passages over ~200 days. OFEC-17FEN cells had a doubling time of 114.34 h and modal diploid chromosome number was 48. The pluripotency genes POU5f1 an...

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Detalles Bibliográficos
Autores principales: Kim, Ju-Won, Oh, Bang Geun, Kim, Julan, Kim, Dong-Gyun, Nam, Bo-Hye, Kim, Young-Ok, Park, Jung Youn, Cheong, JaeHun, Kong, Hee Jeong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Developmental Biology 2018
Materias:
Original Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182235/
https://www.ncbi.nlm.nih.gov/pubmed/30324159
http://dx.doi.org/10.12717/DR.2018.22.3.225
Descripción
Sumario:A new embryonic cell line (OFEC-17FEN) derived from olive flounder Paralichthys olivaceus was developed. OFEC-17FEN cells were subcultured for <30 passages over ~200 days. OFEC-17FEN cells had a doubling time of 114.34 h and modal diploid chromosome number was 48. The pluripotency genes POU5f1 and NANOG were expressed in OFEC-17FEN cells. However, the lack of several pluripotency-related genes expression indicates that OFEC-17FEN cells are not stem cells. OFEC-17FEN cells transfected with plasmid pEGFP-c1 exhibited a strong green fluorescent signal at 48 h after transfection. Accordingly, OFEC-17FEN cells may be useful for both basic research and biotechnological application.

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