Development and Characterization of a New Cell Line from Olive Flounder Paralichthys olivaceus

A new embryonic cell line (OFEC-17FEN) derived from olive flounder Paralichthys olivaceus was developed. OFEC-17FEN cells were subcultured for <30 passages over ~200 days. OFEC-17FEN cells had a doubling time of 114.34 h and modal diploid chromosome number was 48. The pluripotency genes POU5f1 an...

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Autores principales: Kim, Ju-Won, Oh, Bang Geun, Kim, Julan, Kim, Dong-Gyun, Nam, Bo-Hye, Kim, Young-Ok, Park, Jung Youn, Cheong, JaeHun, Kong, Hee Jeong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Developmental Biology 2018
Materias:
Original Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182235/
https://www.ncbi.nlm.nih.gov/pubmed/30324159
http://dx.doi.org/10.12717/DR.2018.22.3.225
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author Kim, Ju-Won
Oh, Bang Geun
Kim, Julan
Kim, Dong-Gyun
Nam, Bo-Hye
Kim, Young-Ok
Park, Jung Youn
Cheong, JaeHun
Kong, Hee Jeong
author_facet Kim, Ju-Won
Oh, Bang Geun
Kim, Julan
Kim, Dong-Gyun
Nam, Bo-Hye
Kim, Young-Ok
Park, Jung Youn
Cheong, JaeHun
Kong, Hee Jeong
author_sort Kim, Ju-Won
collection PubMed
description A new embryonic cell line (OFEC-17FEN) derived from olive flounder Paralichthys olivaceus was developed. OFEC-17FEN cells were subcultured for <30 passages over ~200 days. OFEC-17FEN cells had a doubling time of 114.34 h and modal diploid chromosome number was 48. The pluripotency genes POU5f1 and NANOG were expressed in OFEC-17FEN cells. However, the lack of several pluripotency-related genes expression indicates that OFEC-17FEN cells are not stem cells. OFEC-17FEN cells transfected with plasmid pEGFP-c1 exhibited a strong green fluorescent signal at 48 h after transfection. Accordingly, OFEC-17FEN cells may be useful for both basic research and biotechnological application.
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spelling pubmed-61822352018-10-15 Development and Characterization of a New Cell Line from Olive Flounder Paralichthys olivaceus Kim, Ju-Won Oh, Bang Geun Kim, Julan Kim, Dong-Gyun Nam, Bo-Hye Kim, Young-Ok Park, Jung Youn Cheong, JaeHun Kong, Hee Jeong Dev Reprod Original Research Paper A new embryonic cell line (OFEC-17FEN) derived from olive flounder Paralichthys olivaceus was developed. OFEC-17FEN cells were subcultured for <30 passages over ~200 days. OFEC-17FEN cells had a doubling time of 114.34 h and modal diploid chromosome number was 48. The pluripotency genes POU5f1 and NANOG were expressed in OFEC-17FEN cells. However, the lack of several pluripotency-related genes expression indicates that OFEC-17FEN cells are not stem cells. OFEC-17FEN cells transfected with plasmid pEGFP-c1 exhibited a strong green fluorescent signal at 48 h after transfection. Accordingly, OFEC-17FEN cells may be useful for both basic research and biotechnological application. Korean Society of Developmental Biology 2018-09 2018-09-30 /pmc/articles/PMC6182235/ /pubmed/30324159 http://dx.doi.org/10.12717/DR.2018.22.3.225 Text en © Copyright 2018 The Korean Society of Developmental Biology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research Paper
Kim, Ju-Won
Oh, Bang Geun
Kim, Julan
Kim, Dong-Gyun
Nam, Bo-Hye
Kim, Young-Ok
Park, Jung Youn
Cheong, JaeHun
Kong, Hee Jeong
Development and Characterization of a New Cell Line from Olive Flounder Paralichthys olivaceus
title Development and Characterization of a New Cell Line from Olive Flounder Paralichthys olivaceus
title_full Development and Characterization of a New Cell Line from Olive Flounder Paralichthys olivaceus
title_fullStr Development and Characterization of a New Cell Line from Olive Flounder Paralichthys olivaceus
title_full_unstemmed Development and Characterization of a New Cell Line from Olive Flounder Paralichthys olivaceus
title_short Development and Characterization of a New Cell Line from Olive Flounder Paralichthys olivaceus
title_sort development and characterization of a new cell line from olive flounder paralichthys olivaceus
topic Original Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182235/
https://www.ncbi.nlm.nih.gov/pubmed/30324159
http://dx.doi.org/10.12717/DR.2018.22.3.225
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