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Genomic and phenotypic description of the newly isolated human species Collinsella bouchesdurhonensis sp. nov.

Using culturomics, a recently developed strategy based on diversified culture conditions for the isolation of previously uncultured bacteria, we isolated strain Marseille‐P3296(T) from a fecal sample of a healthy pygmy female. A multiphasic approach, taxono‐genomics, was used to describe the major c...

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Autores principales: Bilen, Melhem, Beye, Mamadou, Mbogning Fonkou, Maxime Descartes, Khelaifia, Saber, Cadoret, Frédéric, Armstrong, Nicholas, Nguyen, Thi Tien, Delerce, Jérémy, Daoud, Ziad, Raoult, Didier, Fournier, Pierre‐Edouard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182551/
https://www.ncbi.nlm.nih.gov/pubmed/29900684
http://dx.doi.org/10.1002/mbo3.580
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author Bilen, Melhem
Beye, Mamadou
Mbogning Fonkou, Maxime Descartes
Khelaifia, Saber
Cadoret, Frédéric
Armstrong, Nicholas
Nguyen, Thi Tien
Delerce, Jérémy
Daoud, Ziad
Raoult, Didier
Fournier, Pierre‐Edouard
author_facet Bilen, Melhem
Beye, Mamadou
Mbogning Fonkou, Maxime Descartes
Khelaifia, Saber
Cadoret, Frédéric
Armstrong, Nicholas
Nguyen, Thi Tien
Delerce, Jérémy
Daoud, Ziad
Raoult, Didier
Fournier, Pierre‐Edouard
author_sort Bilen, Melhem
collection PubMed
description Using culturomics, a recently developed strategy based on diversified culture conditions for the isolation of previously uncultured bacteria, we isolated strain Marseille‐P3296(T) from a fecal sample of a healthy pygmy female. A multiphasic approach, taxono‐genomics, was used to describe the major characteristics of this anaerobic and gram‐positive bacillus that is unable to sporulate and is not motile. The genome of this bacterium is 1,878,572 bp‐long with a 57.94 mol% G + C content. On the basis of these characteristics and after comparison with its closest phylogenetic neighbors, we are confident that strain Marseille‐P3296(T) (=CCUG 70328 =  CSUR P3296) is the type strain of a novel species for which we propose the name Collinsella bouchesdurhonensis sp. nov.
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spelling pubmed-61825512018-10-19 Genomic and phenotypic description of the newly isolated human species Collinsella bouchesdurhonensis sp. nov. Bilen, Melhem Beye, Mamadou Mbogning Fonkou, Maxime Descartes Khelaifia, Saber Cadoret, Frédéric Armstrong, Nicholas Nguyen, Thi Tien Delerce, Jérémy Daoud, Ziad Raoult, Didier Fournier, Pierre‐Edouard Microbiologyopen Original Articles Using culturomics, a recently developed strategy based on diversified culture conditions for the isolation of previously uncultured bacteria, we isolated strain Marseille‐P3296(T) from a fecal sample of a healthy pygmy female. A multiphasic approach, taxono‐genomics, was used to describe the major characteristics of this anaerobic and gram‐positive bacillus that is unable to sporulate and is not motile. The genome of this bacterium is 1,878,572 bp‐long with a 57.94 mol% G + C content. On the basis of these characteristics and after comparison with its closest phylogenetic neighbors, we are confident that strain Marseille‐P3296(T) (=CCUG 70328 =  CSUR P3296) is the type strain of a novel species for which we propose the name Collinsella bouchesdurhonensis sp. nov. John Wiley and Sons Inc. 2018-06-13 /pmc/articles/PMC6182551/ /pubmed/29900684 http://dx.doi.org/10.1002/mbo3.580 Text en © 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Bilen, Melhem
Beye, Mamadou
Mbogning Fonkou, Maxime Descartes
Khelaifia, Saber
Cadoret, Frédéric
Armstrong, Nicholas
Nguyen, Thi Tien
Delerce, Jérémy
Daoud, Ziad
Raoult, Didier
Fournier, Pierre‐Edouard
Genomic and phenotypic description of the newly isolated human species Collinsella bouchesdurhonensis sp. nov.
title Genomic and phenotypic description of the newly isolated human species Collinsella bouchesdurhonensis sp. nov.
title_full Genomic and phenotypic description of the newly isolated human species Collinsella bouchesdurhonensis sp. nov.
title_fullStr Genomic and phenotypic description of the newly isolated human species Collinsella bouchesdurhonensis sp. nov.
title_full_unstemmed Genomic and phenotypic description of the newly isolated human species Collinsella bouchesdurhonensis sp. nov.
title_short Genomic and phenotypic description of the newly isolated human species Collinsella bouchesdurhonensis sp. nov.
title_sort genomic and phenotypic description of the newly isolated human species collinsella bouchesdurhonensis sp. nov.
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182551/
https://www.ncbi.nlm.nih.gov/pubmed/29900684
http://dx.doi.org/10.1002/mbo3.580
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