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Morphological evolution of various fungal species in the presence and absence of aluminum oxide microparticles: Comparative and quantitative insights into microparticle‐enhanced cultivation (MPEC)

The application of microparticle‐enhanced cultivation (MPEC) is an attractive method to control mycelial morphology, and thus enhance the production of metabolites and enzymes in the submerged cultivations of filamentous fungi. Unfortunately, most literature data deals with the spore‐agglomerating s...

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Autores principales: Kowalska, Anna, Boruta, Tomasz, Bizukojć, Marcin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182563/
https://www.ncbi.nlm.nih.gov/pubmed/29504287
http://dx.doi.org/10.1002/mbo3.603
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author Kowalska, Anna
Boruta, Tomasz
Bizukojć, Marcin
author_facet Kowalska, Anna
Boruta, Tomasz
Bizukojć, Marcin
author_sort Kowalska, Anna
collection PubMed
description The application of microparticle‐enhanced cultivation (MPEC) is an attractive method to control mycelial morphology, and thus enhance the production of metabolites and enzymes in the submerged cultivations of filamentous fungi. Unfortunately, most literature data deals with the spore‐agglomerating species like aspergilli. Therefore, the detailed quantitative study of the morphological evolution of four different fungal species (Aspergillus terreus, Penicillium rubens, Chaetomium globosum, and Mucor racemosus) based on the digital analysis of microscopic images was presented in this paper. In accordance with the current knowledge, these species exhibit different mechanisms of agglomerates formation. The standard submerged shake flask cultivations (as a reference) and MPEC involving 10 μm aluminum oxide microparticles (6 g·L(−1)) were performed. The morphological parameters, including mean projected area, elongation, roughness, and morphology number were determined for the mycelial objects within the first 24 hr of growth. It occurred that heretofore observed and widely discussed effect of microparticles on fungi, namely the decrease in pellet size, was not observed for the species whose pellet formation mechanism is different from spore agglomeration. In the MPEC, C. globosum developed core‐shell pellets, and M. racemosus, a nonagglomerative species, formed the relatively larger, compared to standard cultures, pellets with distinct cores.
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spelling pubmed-61825632018-10-19 Morphological evolution of various fungal species in the presence and absence of aluminum oxide microparticles: Comparative and quantitative insights into microparticle‐enhanced cultivation (MPEC) Kowalska, Anna Boruta, Tomasz Bizukojć, Marcin Microbiologyopen Original Articles The application of microparticle‐enhanced cultivation (MPEC) is an attractive method to control mycelial morphology, and thus enhance the production of metabolites and enzymes in the submerged cultivations of filamentous fungi. Unfortunately, most literature data deals with the spore‐agglomerating species like aspergilli. Therefore, the detailed quantitative study of the morphological evolution of four different fungal species (Aspergillus terreus, Penicillium rubens, Chaetomium globosum, and Mucor racemosus) based on the digital analysis of microscopic images was presented in this paper. In accordance with the current knowledge, these species exhibit different mechanisms of agglomerates formation. The standard submerged shake flask cultivations (as a reference) and MPEC involving 10 μm aluminum oxide microparticles (6 g·L(−1)) were performed. The morphological parameters, including mean projected area, elongation, roughness, and morphology number were determined for the mycelial objects within the first 24 hr of growth. It occurred that heretofore observed and widely discussed effect of microparticles on fungi, namely the decrease in pellet size, was not observed for the species whose pellet formation mechanism is different from spore agglomeration. In the MPEC, C. globosum developed core‐shell pellets, and M. racemosus, a nonagglomerative species, formed the relatively larger, compared to standard cultures, pellets with distinct cores. John Wiley and Sons Inc. 2018-03-05 /pmc/articles/PMC6182563/ /pubmed/29504287 http://dx.doi.org/10.1002/mbo3.603 Text en © 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Kowalska, Anna
Boruta, Tomasz
Bizukojć, Marcin
Morphological evolution of various fungal species in the presence and absence of aluminum oxide microparticles: Comparative and quantitative insights into microparticle‐enhanced cultivation (MPEC)
title Morphological evolution of various fungal species in the presence and absence of aluminum oxide microparticles: Comparative and quantitative insights into microparticle‐enhanced cultivation (MPEC)
title_full Morphological evolution of various fungal species in the presence and absence of aluminum oxide microparticles: Comparative and quantitative insights into microparticle‐enhanced cultivation (MPEC)
title_fullStr Morphological evolution of various fungal species in the presence and absence of aluminum oxide microparticles: Comparative and quantitative insights into microparticle‐enhanced cultivation (MPEC)
title_full_unstemmed Morphological evolution of various fungal species in the presence and absence of aluminum oxide microparticles: Comparative and quantitative insights into microparticle‐enhanced cultivation (MPEC)
title_short Morphological evolution of various fungal species in the presence and absence of aluminum oxide microparticles: Comparative and quantitative insights into microparticle‐enhanced cultivation (MPEC)
title_sort morphological evolution of various fungal species in the presence and absence of aluminum oxide microparticles: comparative and quantitative insights into microparticle‐enhanced cultivation (mpec)
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182563/
https://www.ncbi.nlm.nih.gov/pubmed/29504287
http://dx.doi.org/10.1002/mbo3.603
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