MLST and RAPD molecular analysis of Staphylococcus aureus subsp. anaerobius isolated from goats in Poland

Staphylococcus aureus subsp. anaerobius is an etiological agent of Morel’s disease in small ruminants. The infection results in superficial abscesses located near lymph nodes. In the study, molecular analysis based on multilocus sequence typing (MLST) of seven housekeeping genes (arcC, aroE, glp, gm...

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Autores principales: Szaluś-Jordanow, Olga, Krysztopa-Grzybowska, Katarzyna, Czopowicz, Michał, Moroz, Agata, Mickiewicz, Marcin, Lutyńska, Anna, Kaba, Jarosław, Nalbert, Tomasz, Frymus, Tadeusz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2018
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Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182609/
https://www.ncbi.nlm.nih.gov/pubmed/30182255
http://dx.doi.org/10.1007/s00203-018-1568-1
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author Szaluś-Jordanow, Olga
Krysztopa-Grzybowska, Katarzyna
Czopowicz, Michał
Moroz, Agata
Mickiewicz, Marcin
Lutyńska, Anna
Kaba, Jarosław
Nalbert, Tomasz
Frymus, Tadeusz
author_facet Szaluś-Jordanow, Olga
Krysztopa-Grzybowska, Katarzyna
Czopowicz, Michał
Moroz, Agata
Mickiewicz, Marcin
Lutyńska, Anna
Kaba, Jarosław
Nalbert, Tomasz
Frymus, Tadeusz
author_sort Szaluś-Jordanow, Olga
collection PubMed
description Staphylococcus aureus subsp. anaerobius is an etiological agent of Morel’s disease in small ruminants. The infection results in superficial abscesses located near lymph nodes. In the study, molecular analysis based on multilocus sequence typing (MLST) of seven housekeeping genes (arcC, aroE, glp, gmk, pta, tpi, yqiL) and random amplified polymorphic DNA (RAPD) was carried out on 19 S. aureus subsp. anaerobius strains isolated from two different goat herds from Poland. All of the 19 S. aureus subsp. anaerobius strains were found to belong to single MLST and RAPD types which support the high clonality level of this agent. However, the results obtained show clearly that the S. aureus subsp. anaerobius clone found in goats in Poland is different from those previously described. However, it is identical to the ATCC 38844 strain isolated from sheep in Spain, which has not been so far genotyped using MLST.
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spelling pubmed-61826092018-10-22 MLST and RAPD molecular analysis of Staphylococcus aureus subsp. anaerobius isolated from goats in Poland Szaluś-Jordanow, Olga Krysztopa-Grzybowska, Katarzyna Czopowicz, Michał Moroz, Agata Mickiewicz, Marcin Lutyńska, Anna Kaba, Jarosław Nalbert, Tomasz Frymus, Tadeusz Arch Microbiol Short Communication Staphylococcus aureus subsp. anaerobius is an etiological agent of Morel’s disease in small ruminants. The infection results in superficial abscesses located near lymph nodes. In the study, molecular analysis based on multilocus sequence typing (MLST) of seven housekeeping genes (arcC, aroE, glp, gmk, pta, tpi, yqiL) and random amplified polymorphic DNA (RAPD) was carried out on 19 S. aureus subsp. anaerobius strains isolated from two different goat herds from Poland. All of the 19 S. aureus subsp. anaerobius strains were found to belong to single MLST and RAPD types which support the high clonality level of this agent. However, the results obtained show clearly that the S. aureus subsp. anaerobius clone found in goats in Poland is different from those previously described. However, it is identical to the ATCC 38844 strain isolated from sheep in Spain, which has not been so far genotyped using MLST. Springer Berlin Heidelberg 2018-09-04 2018 /pmc/articles/PMC6182609/ /pubmed/30182255 http://dx.doi.org/10.1007/s00203-018-1568-1 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Short Communication
Szaluś-Jordanow, Olga
Krysztopa-Grzybowska, Katarzyna
Czopowicz, Michał
Moroz, Agata
Mickiewicz, Marcin
Lutyńska, Anna
Kaba, Jarosław
Nalbert, Tomasz
Frymus, Tadeusz
MLST and RAPD molecular analysis of Staphylococcus aureus subsp. anaerobius isolated from goats in Poland
title MLST and RAPD molecular analysis of Staphylococcus aureus subsp. anaerobius isolated from goats in Poland
title_full MLST and RAPD molecular analysis of Staphylococcus aureus subsp. anaerobius isolated from goats in Poland
title_fullStr MLST and RAPD molecular analysis of Staphylococcus aureus subsp. anaerobius isolated from goats in Poland
title_full_unstemmed MLST and RAPD molecular analysis of Staphylococcus aureus subsp. anaerobius isolated from goats in Poland
title_short MLST and RAPD molecular analysis of Staphylococcus aureus subsp. anaerobius isolated from goats in Poland
title_sort mlst and rapd molecular analysis of staphylococcus aureus subsp. anaerobius isolated from goats in poland
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182609/
https://www.ncbi.nlm.nih.gov/pubmed/30182255
http://dx.doi.org/10.1007/s00203-018-1568-1
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