Cargando…
Surveillance of transmitted HIV-1 antiretroviral drug resistance in the context of decentralized HIV care in Senegal and the Ebola outbreak in Guinea
OBJECTIVES: Disruption in HIV care provision may enhance the development and spread of drug resistance due to inadequate antiretroviral therapy. This study thus determined the prevalence of HIV-1 transmitted drug resistance (TDR) in settings of decentralized therapy and care in Senegal and, the Ebol...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182815/ https://www.ncbi.nlm.nih.gov/pubmed/30309385 http://dx.doi.org/10.1186/s13104-018-3804-9 |
_version_ | 1783362652015165440 |
---|---|
author | Mbange, Aristid Ekollo Kaba, Djiba Diouara, Abou Abdallah Malick Diop-Ndiaye, Halimatou Ngom-Ngueye, Ndeye Fatou Dieng, Ahmed Lo, Seynabou Toure, Kine Ndiaye Fall, Mamadou Mbacham, Wilfred Fon Diallo, Mariama Sadjo Cisse, Mohamed Mboup, Souleymane Kane, Coumba Toure |
author_facet | Mbange, Aristid Ekollo Kaba, Djiba Diouara, Abou Abdallah Malick Diop-Ndiaye, Halimatou Ngom-Ngueye, Ndeye Fatou Dieng, Ahmed Lo, Seynabou Toure, Kine Ndiaye Fall, Mamadou Mbacham, Wilfred Fon Diallo, Mariama Sadjo Cisse, Mohamed Mboup, Souleymane Kane, Coumba Toure |
author_sort | Mbange, Aristid Ekollo |
collection | PubMed |
description | OBJECTIVES: Disruption in HIV care provision may enhance the development and spread of drug resistance due to inadequate antiretroviral therapy. This study thus determined the prevalence of HIV-1 transmitted drug resistance (TDR) in settings of decentralized therapy and care in Senegal and, the Ebola outbreak in Guinea. Antiretroviral-naïve patients were enrolled following a modified WHO TDR Threshold Survey method, implemented in Senegal (January–March 2015) and Guinea (August–September 2015). Plasma and dried blood spots specimens, respectively from Senegalese (n = 69) and Guinean (n = 50) patients, were collected for direct sequencing of HIV-1 pol genes. The Stanford Calibrated Population Resistance program v6.0 was used for Surveillance Drug Resistance Mutations (SDRMs). RESULTS: Genotyping was successful from 54/69 (78.2%) and 31/50 (62.0%) isolates. In Senegal, TDR prevalence was 0% (mean duration since HIV diagnosis 4.08 ± 3.53 years). In Guinea, two patients exhibited SDRMs M184V (NRTI), T215F (TAM) and, G190A (NNRTI), respectively. TDR prevalence at this second site, however, could not be ascertained because of low sample size. Phylogenetic inference confirmed CRF02_AG predominance in Senegal (62.96%) and Guinea (77.42%). TDR prevalence in Senegal remains extremely low suggesting improved control measures. Continuous surveillance in both settings is mandatory and, should be done closest to diagnosis/transmission time and with larger sample size. |
format | Online Article Text |
id | pubmed-6182815 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-61828152018-10-18 Surveillance of transmitted HIV-1 antiretroviral drug resistance in the context of decentralized HIV care in Senegal and the Ebola outbreak in Guinea Mbange, Aristid Ekollo Kaba, Djiba Diouara, Abou Abdallah Malick Diop-Ndiaye, Halimatou Ngom-Ngueye, Ndeye Fatou Dieng, Ahmed Lo, Seynabou Toure, Kine Ndiaye Fall, Mamadou Mbacham, Wilfred Fon Diallo, Mariama Sadjo Cisse, Mohamed Mboup, Souleymane Kane, Coumba Toure BMC Res Notes Research Note OBJECTIVES: Disruption in HIV care provision may enhance the development and spread of drug resistance due to inadequate antiretroviral therapy. This study thus determined the prevalence of HIV-1 transmitted drug resistance (TDR) in settings of decentralized therapy and care in Senegal and, the Ebola outbreak in Guinea. Antiretroviral-naïve patients were enrolled following a modified WHO TDR Threshold Survey method, implemented in Senegal (January–March 2015) and Guinea (August–September 2015). Plasma and dried blood spots specimens, respectively from Senegalese (n = 69) and Guinean (n = 50) patients, were collected for direct sequencing of HIV-1 pol genes. The Stanford Calibrated Population Resistance program v6.0 was used for Surveillance Drug Resistance Mutations (SDRMs). RESULTS: Genotyping was successful from 54/69 (78.2%) and 31/50 (62.0%) isolates. In Senegal, TDR prevalence was 0% (mean duration since HIV diagnosis 4.08 ± 3.53 years). In Guinea, two patients exhibited SDRMs M184V (NRTI), T215F (TAM) and, G190A (NNRTI), respectively. TDR prevalence at this second site, however, could not be ascertained because of low sample size. Phylogenetic inference confirmed CRF02_AG predominance in Senegal (62.96%) and Guinea (77.42%). TDR prevalence in Senegal remains extremely low suggesting improved control measures. Continuous surveillance in both settings is mandatory and, should be done closest to diagnosis/transmission time and with larger sample size. BioMed Central 2018-10-12 /pmc/articles/PMC6182815/ /pubmed/30309385 http://dx.doi.org/10.1186/s13104-018-3804-9 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Note Mbange, Aristid Ekollo Kaba, Djiba Diouara, Abou Abdallah Malick Diop-Ndiaye, Halimatou Ngom-Ngueye, Ndeye Fatou Dieng, Ahmed Lo, Seynabou Toure, Kine Ndiaye Fall, Mamadou Mbacham, Wilfred Fon Diallo, Mariama Sadjo Cisse, Mohamed Mboup, Souleymane Kane, Coumba Toure Surveillance of transmitted HIV-1 antiretroviral drug resistance in the context of decentralized HIV care in Senegal and the Ebola outbreak in Guinea |
title | Surveillance of transmitted HIV-1 antiretroviral drug resistance in the context of decentralized HIV care in Senegal and the Ebola outbreak in Guinea |
title_full | Surveillance of transmitted HIV-1 antiretroviral drug resistance in the context of decentralized HIV care in Senegal and the Ebola outbreak in Guinea |
title_fullStr | Surveillance of transmitted HIV-1 antiretroviral drug resistance in the context of decentralized HIV care in Senegal and the Ebola outbreak in Guinea |
title_full_unstemmed | Surveillance of transmitted HIV-1 antiretroviral drug resistance in the context of decentralized HIV care in Senegal and the Ebola outbreak in Guinea |
title_short | Surveillance of transmitted HIV-1 antiretroviral drug resistance in the context of decentralized HIV care in Senegal and the Ebola outbreak in Guinea |
title_sort | surveillance of transmitted hiv-1 antiretroviral drug resistance in the context of decentralized hiv care in senegal and the ebola outbreak in guinea |
topic | Research Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182815/ https://www.ncbi.nlm.nih.gov/pubmed/30309385 http://dx.doi.org/10.1186/s13104-018-3804-9 |
work_keys_str_mv | AT mbangearistidekollo surveillanceoftransmittedhiv1antiretroviraldrugresistanceinthecontextofdecentralizedhivcareinsenegalandtheebolaoutbreakinguinea AT kabadjiba surveillanceoftransmittedhiv1antiretroviraldrugresistanceinthecontextofdecentralizedhivcareinsenegalandtheebolaoutbreakinguinea AT diouaraabouabdallahmalick surveillanceoftransmittedhiv1antiretroviraldrugresistanceinthecontextofdecentralizedhivcareinsenegalandtheebolaoutbreakinguinea AT diopndiayehalimatou surveillanceoftransmittedhiv1antiretroviraldrugresistanceinthecontextofdecentralizedhivcareinsenegalandtheebolaoutbreakinguinea AT ngomngueyendeyefatou surveillanceoftransmittedhiv1antiretroviraldrugresistanceinthecontextofdecentralizedhivcareinsenegalandtheebolaoutbreakinguinea AT diengahmed surveillanceoftransmittedhiv1antiretroviraldrugresistanceinthecontextofdecentralizedhivcareinsenegalandtheebolaoutbreakinguinea AT loseynabou surveillanceoftransmittedhiv1antiretroviraldrugresistanceinthecontextofdecentralizedhivcareinsenegalandtheebolaoutbreakinguinea AT tourekinendiaye surveillanceoftransmittedhiv1antiretroviraldrugresistanceinthecontextofdecentralizedhivcareinsenegalandtheebolaoutbreakinguinea AT fallmamadou surveillanceoftransmittedhiv1antiretroviraldrugresistanceinthecontextofdecentralizedhivcareinsenegalandtheebolaoutbreakinguinea AT mbachamwilfredfon surveillanceoftransmittedhiv1antiretroviraldrugresistanceinthecontextofdecentralizedhivcareinsenegalandtheebolaoutbreakinguinea AT diallomariamasadjo surveillanceoftransmittedhiv1antiretroviraldrugresistanceinthecontextofdecentralizedhivcareinsenegalandtheebolaoutbreakinguinea AT cissemohamed surveillanceoftransmittedhiv1antiretroviraldrugresistanceinthecontextofdecentralizedhivcareinsenegalandtheebolaoutbreakinguinea AT mboupsouleymane surveillanceoftransmittedhiv1antiretroviraldrugresistanceinthecontextofdecentralizedhivcareinsenegalandtheebolaoutbreakinguinea AT kanecoumbatoure surveillanceoftransmittedhiv1antiretroviraldrugresistanceinthecontextofdecentralizedhivcareinsenegalandtheebolaoutbreakinguinea |