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Quantitative dissection of variations in root growth rate: a matter of cell proliferation or of cell expansion?
Plant organ growth results from cell production and cell expansion. Deciphering the contribution of each of these processes to growth rate is an important issue in developmental biology. Here, we investigated the cellular processes governing root elongation rate, considering two sources of variation...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6184812/ https://www.ncbi.nlm.nih.gov/pubmed/30053124 http://dx.doi.org/10.1093/jxb/ery272 |
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author | Youssef, Chvan Bizet, François Bastien, Renaud Legland, David Bogeat-Triboulot, Marie-Béatrice Hummel, Irène |
author_facet | Youssef, Chvan Bizet, François Bastien, Renaud Legland, David Bogeat-Triboulot, Marie-Béatrice Hummel, Irène |
author_sort | Youssef, Chvan |
collection | PubMed |
description | Plant organ growth results from cell production and cell expansion. Deciphering the contribution of each of these processes to growth rate is an important issue in developmental biology. Here, we investigated the cellular processes governing root elongation rate, considering two sources of variation: genotype and disturbance by chemicals (NaCl, polyethylene glycol, H(2)O(2), abscisic acid). Exploiting the adventitious rooting capacity of the Populus genus, and using time-lapse imaging under infrared-light, particle image velocimetry, histological analysis, and kinematics, we quantified the cellular processes involved in root growth variation, and analysed the covariation patterns between growth parameters. The rate of cell production by the root apical meristem and the number of dividing cells were estimated in vivo without destructive measurement. We found that the rate of cell division contributed more to the variation in cell production rate than the number of dividing cells. Regardless of the source of variation, the length of the elongation zone was the best proxy for growth rate, summarizing rates of cell production and cell elongation into a single parameter. Our results demonstrate that cell production rate is the main driver of growth rate, whereas elemental elongation rate is a key driver of short-term growth adjustments. |
format | Online Article Text |
id | pubmed-6184812 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-61848122018-10-18 Quantitative dissection of variations in root growth rate: a matter of cell proliferation or of cell expansion? Youssef, Chvan Bizet, François Bastien, Renaud Legland, David Bogeat-Triboulot, Marie-Béatrice Hummel, Irène J Exp Bot Research Papers Plant organ growth results from cell production and cell expansion. Deciphering the contribution of each of these processes to growth rate is an important issue in developmental biology. Here, we investigated the cellular processes governing root elongation rate, considering two sources of variation: genotype and disturbance by chemicals (NaCl, polyethylene glycol, H(2)O(2), abscisic acid). Exploiting the adventitious rooting capacity of the Populus genus, and using time-lapse imaging under infrared-light, particle image velocimetry, histological analysis, and kinematics, we quantified the cellular processes involved in root growth variation, and analysed the covariation patterns between growth parameters. The rate of cell production by the root apical meristem and the number of dividing cells were estimated in vivo without destructive measurement. We found that the rate of cell division contributed more to the variation in cell production rate than the number of dividing cells. Regardless of the source of variation, the length of the elongation zone was the best proxy for growth rate, summarizing rates of cell production and cell elongation into a single parameter. Our results demonstrate that cell production rate is the main driver of growth rate, whereas elemental elongation rate is a key driver of short-term growth adjustments. Oxford University Press 2018-10-12 2018-07-25 /pmc/articles/PMC6184812/ /pubmed/30053124 http://dx.doi.org/10.1093/jxb/ery272 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Papers Youssef, Chvan Bizet, François Bastien, Renaud Legland, David Bogeat-Triboulot, Marie-Béatrice Hummel, Irène Quantitative dissection of variations in root growth rate: a matter of cell proliferation or of cell expansion? |
title | Quantitative dissection of variations in root growth rate: a matter of cell proliferation or of cell expansion? |
title_full | Quantitative dissection of variations in root growth rate: a matter of cell proliferation or of cell expansion? |
title_fullStr | Quantitative dissection of variations in root growth rate: a matter of cell proliferation or of cell expansion? |
title_full_unstemmed | Quantitative dissection of variations in root growth rate: a matter of cell proliferation or of cell expansion? |
title_short | Quantitative dissection of variations in root growth rate: a matter of cell proliferation or of cell expansion? |
title_sort | quantitative dissection of variations in root growth rate: a matter of cell proliferation or of cell expansion? |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6184812/ https://www.ncbi.nlm.nih.gov/pubmed/30053124 http://dx.doi.org/10.1093/jxb/ery272 |
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