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Draft genome sequences of Cylindrospermopsis raciborskii strains CS-508 and MVCC14, isolated from freshwater bloom events in Australia and Uruguay

Members of the genus Cylindrospermopsis represent an important environmental and health concern. Strains CS-508 and MVCC14 of C. raciborskii were isolated from freshwater reservoirs located in Australia and Uruguay, respectively. While CS-508 has been reported as non-toxic, MVCC14 is a saxitoxin (ST...

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Autores principales: Fuentes-Valdés, Juan J, Soto-Liebe, Katia, Pérez-Pantoja, Danilo, Tamames, Javier, Belmar, Lucy, Pedrós-Alió, Carlos, Garrido, Daniel, Vásquez, Mónica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6186047/
https://www.ncbi.nlm.nih.gov/pubmed/30344889
http://dx.doi.org/10.1186/s40793-018-0323-1
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author Fuentes-Valdés, Juan J
Soto-Liebe, Katia
Pérez-Pantoja, Danilo
Tamames, Javier
Belmar, Lucy
Pedrós-Alió, Carlos
Garrido, Daniel
Vásquez, Mónica
author_facet Fuentes-Valdés, Juan J
Soto-Liebe, Katia
Pérez-Pantoja, Danilo
Tamames, Javier
Belmar, Lucy
Pedrós-Alió, Carlos
Garrido, Daniel
Vásquez, Mónica
author_sort Fuentes-Valdés, Juan J
collection PubMed
description Members of the genus Cylindrospermopsis represent an important environmental and health concern. Strains CS-508 and MVCC14 of C. raciborskii were isolated from freshwater reservoirs located in Australia and Uruguay, respectively. While CS-508 has been reported as non-toxic, MVCC14 is a saxitoxin (STX) producer. We annotated the draft genomes of these C. raciborskii strains using the assembly of reads obtained from Illumina MiSeq sequencing. The final assemblies resulted in genome sizes close to 3.6 Mbp for both strains and included 3202 ORFs for CS-508 (in 163 contigs) and 3560 ORFs for MVCC14 (in 99 contigs). Finally, both the average nucleotide identity (ANI) and the similarity of gene content indicate that these two genomes should be considered as strains of the C. raciborskii species. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s40793-018-0323-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-61860472018-10-19 Draft genome sequences of Cylindrospermopsis raciborskii strains CS-508 and MVCC14, isolated from freshwater bloom events in Australia and Uruguay Fuentes-Valdés, Juan J Soto-Liebe, Katia Pérez-Pantoja, Danilo Tamames, Javier Belmar, Lucy Pedrós-Alió, Carlos Garrido, Daniel Vásquez, Mónica Stand Genomic Sci Short Genome Report Members of the genus Cylindrospermopsis represent an important environmental and health concern. Strains CS-508 and MVCC14 of C. raciborskii were isolated from freshwater reservoirs located in Australia and Uruguay, respectively. While CS-508 has been reported as non-toxic, MVCC14 is a saxitoxin (STX) producer. We annotated the draft genomes of these C. raciborskii strains using the assembly of reads obtained from Illumina MiSeq sequencing. The final assemblies resulted in genome sizes close to 3.6 Mbp for both strains and included 3202 ORFs for CS-508 (in 163 contigs) and 3560 ORFs for MVCC14 (in 99 contigs). Finally, both the average nucleotide identity (ANI) and the similarity of gene content indicate that these two genomes should be considered as strains of the C. raciborskii species. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s40793-018-0323-1) contains supplementary material, which is available to authorized users. BioMed Central 2018-10-12 /pmc/articles/PMC6186047/ /pubmed/30344889 http://dx.doi.org/10.1186/s40793-018-0323-1 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Genome Report
Fuentes-Valdés, Juan J
Soto-Liebe, Katia
Pérez-Pantoja, Danilo
Tamames, Javier
Belmar, Lucy
Pedrós-Alió, Carlos
Garrido, Daniel
Vásquez, Mónica
Draft genome sequences of Cylindrospermopsis raciborskii strains CS-508 and MVCC14, isolated from freshwater bloom events in Australia and Uruguay
title Draft genome sequences of Cylindrospermopsis raciborskii strains CS-508 and MVCC14, isolated from freshwater bloom events in Australia and Uruguay
title_full Draft genome sequences of Cylindrospermopsis raciborskii strains CS-508 and MVCC14, isolated from freshwater bloom events in Australia and Uruguay
title_fullStr Draft genome sequences of Cylindrospermopsis raciborskii strains CS-508 and MVCC14, isolated from freshwater bloom events in Australia and Uruguay
title_full_unstemmed Draft genome sequences of Cylindrospermopsis raciborskii strains CS-508 and MVCC14, isolated from freshwater bloom events in Australia and Uruguay
title_short Draft genome sequences of Cylindrospermopsis raciborskii strains CS-508 and MVCC14, isolated from freshwater bloom events in Australia and Uruguay
title_sort draft genome sequences of cylindrospermopsis raciborskii strains cs-508 and mvcc14, isolated from freshwater bloom events in australia and uruguay
topic Short Genome Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6186047/
https://www.ncbi.nlm.nih.gov/pubmed/30344889
http://dx.doi.org/10.1186/s40793-018-0323-1
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