Novel codons in rat Pdx-1 complementary DNA

OBJECTIVES: Pancreatic and duodenal homeobox-1 (Pdx-1) is a homeodomain-containing transcription factor essential for pancreatic development, beta-cell differentiation and the maintenance of mature beta cell function. To transfect the expression vectors of Pdx-1 in the mammalian cells, the complemen...

Descripción completa

Detalles Bibliográficos
Autor principal: Kiba, Takayoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6186082/
https://www.ncbi.nlm.nih.gov/pubmed/30314444
http://dx.doi.org/10.1186/s13104-018-3837-0
_version_ 1783362806057271296
author Kiba, Takayoshi
author_facet Kiba, Takayoshi
author_sort Kiba, Takayoshi
collection PubMed
description OBJECTIVES: Pancreatic and duodenal homeobox-1 (Pdx-1) is a homeodomain-containing transcription factor essential for pancreatic development, beta-cell differentiation and the maintenance of mature beta cell function. To transfect the expression vectors of Pdx-1 in the mammalian cells, the complementary DNA (cDNA) of Pdx-1 was conducted. RESULTS: Novel codons and amino acids sequences were detected in rat Pdx-1 cDNA. Comparing the previous reports regarding rat Pdx-1 cDNA, 3 novel codons (ACA141CCA, AAG720CCG, GTT742GCT) were detected. The amino acids sequences based on the detected cDNA sequences confirmed those, which were already available in public databases. The present study described novel codons in rat Pdx-1 cDNA. The results may be useful for an effective research against pancreatic development, regeneration or carcinogenesis regarding Pdx-1 expressions.
format Online
Article
Text
id pubmed-6186082
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-61860822018-10-19 Novel codons in rat Pdx-1 complementary DNA Kiba, Takayoshi BMC Res Notes Research Note OBJECTIVES: Pancreatic and duodenal homeobox-1 (Pdx-1) is a homeodomain-containing transcription factor essential for pancreatic development, beta-cell differentiation and the maintenance of mature beta cell function. To transfect the expression vectors of Pdx-1 in the mammalian cells, the complementary DNA (cDNA) of Pdx-1 was conducted. RESULTS: Novel codons and amino acids sequences were detected in rat Pdx-1 cDNA. Comparing the previous reports regarding rat Pdx-1 cDNA, 3 novel codons (ACA141CCA, AAG720CCG, GTT742GCT) were detected. The amino acids sequences based on the detected cDNA sequences confirmed those, which were already available in public databases. The present study described novel codons in rat Pdx-1 cDNA. The results may be useful for an effective research against pancreatic development, regeneration or carcinogenesis regarding Pdx-1 expressions. BioMed Central 2018-10-12 /pmc/articles/PMC6186082/ /pubmed/30314444 http://dx.doi.org/10.1186/s13104-018-3837-0 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Kiba, Takayoshi
Novel codons in rat Pdx-1 complementary DNA
title Novel codons in rat Pdx-1 complementary DNA
title_full Novel codons in rat Pdx-1 complementary DNA
title_fullStr Novel codons in rat Pdx-1 complementary DNA
title_full_unstemmed Novel codons in rat Pdx-1 complementary DNA
title_short Novel codons in rat Pdx-1 complementary DNA
title_sort novel codons in rat pdx-1 complementary dna
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6186082/
https://www.ncbi.nlm.nih.gov/pubmed/30314444
http://dx.doi.org/10.1186/s13104-018-3837-0
work_keys_str_mv AT kibatakayoshi novelcodonsinratpdx1complementarydna

Ejemplares similares