Time- and frequency-resolved fluorescence with a single TCSPC detector via a Fourier-transform approach

We introduce a broadband single-pixel spectro-temporal fluorescence detector, combining time-correlated single photon counting (TCSPC) with Fourier transform (FT) spectroscopy. A birefringent common-path interferometer (CPI) generates two time-delayed replicas of the sample’s fluorescence. Via FT of...

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Detalles Bibliográficos
Autores principales: Perri, Antonio, Gaida, John H., Farina, Andrea, Preda, Fabrizio, Viola, Daniele, Ballottari, Matteo, Hauer, Jürgen, De Silvestri, Sandro, D’Andrea, Cosimo, Cerullo, Giulio, Polli, Dario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6186413/
https://www.ncbi.nlm.nih.gov/pubmed/29401767
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author Perri, Antonio
Gaida, John H.
Farina, Andrea
Preda, Fabrizio
Viola, Daniele
Ballottari, Matteo
Hauer, Jürgen
De Silvestri, Sandro
D’Andrea, Cosimo
Cerullo, Giulio
Polli, Dario
author_facet Perri, Antonio
Gaida, John H.
Farina, Andrea
Preda, Fabrizio
Viola, Daniele
Ballottari, Matteo
Hauer, Jürgen
De Silvestri, Sandro
D’Andrea, Cosimo
Cerullo, Giulio
Polli, Dario
author_sort Perri, Antonio
collection PubMed
description We introduce a broadband single-pixel spectro-temporal fluorescence detector, combining time-correlated single photon counting (TCSPC) with Fourier transform (FT) spectroscopy. A birefringent common-path interferometer (CPI) generates two time-delayed replicas of the sample’s fluorescence. Via FT of their interference signal at the detector, we obtain a two-dimensional map of the fluorescence as a function of detection wavelength and emission time, with high temporal and spectral resolution. Our instrument is remarkably simple, as it only requires the addition of a CPI to a standard single-pixel TCSPC system, and it shows a readily adjustable spectral resolution with inherently broad bandwidth coverage.
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spelling pubmed-61864132018-10-14 Time- and frequency-resolved fluorescence with a single TCSPC detector via a Fourier-transform approach Perri, Antonio Gaida, John H. Farina, Andrea Preda, Fabrizio Viola, Daniele Ballottari, Matteo Hauer, Jürgen De Silvestri, Sandro D’Andrea, Cosimo Cerullo, Giulio Polli, Dario Opt Express Article We introduce a broadband single-pixel spectro-temporal fluorescence detector, combining time-correlated single photon counting (TCSPC) with Fourier transform (FT) spectroscopy. A birefringent common-path interferometer (CPI) generates two time-delayed replicas of the sample’s fluorescence. Via FT of their interference signal at the detector, we obtain a two-dimensional map of the fluorescence as a function of detection wavelength and emission time, with high temporal and spectral resolution. Our instrument is remarkably simple, as it only requires the addition of a CPI to a standard single-pixel TCSPC system, and it shows a readily adjustable spectral resolution with inherently broad bandwidth coverage. 2018-02-05 /pmc/articles/PMC6186413/ /pubmed/29401767 Text en https://creativecommons.org/licenses/by/4.0/ Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) . Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.
spellingShingle Article
Perri, Antonio
Gaida, John H.
Farina, Andrea
Preda, Fabrizio
Viola, Daniele
Ballottari, Matteo
Hauer, Jürgen
De Silvestri, Sandro
D’Andrea, Cosimo
Cerullo, Giulio
Polli, Dario
Time- and frequency-resolved fluorescence with a single TCSPC detector via a Fourier-transform approach
title Time- and frequency-resolved fluorescence with a single TCSPC detector via a Fourier-transform approach
title_full Time- and frequency-resolved fluorescence with a single TCSPC detector via a Fourier-transform approach
title_fullStr Time- and frequency-resolved fluorescence with a single TCSPC detector via a Fourier-transform approach
title_full_unstemmed Time- and frequency-resolved fluorescence with a single TCSPC detector via a Fourier-transform approach
title_short Time- and frequency-resolved fluorescence with a single TCSPC detector via a Fourier-transform approach
title_sort time- and frequency-resolved fluorescence with a single tcspc detector via a fourier-transform approach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6186413/
https://www.ncbi.nlm.nih.gov/pubmed/29401767
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