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Adhesion Properties of Food-Associated Lactobacillus plantarum Strains on Human Intestinal Epithelial Cells and Modulation of IL-8 Release

Food-associated microbes can reach the gut as viable cells and interact with the human host providing potential health benefits. In this study, we evaluated the impact on cell viability and the adhesion ability of 22 Lactobacillus plantarum strains, mainly isolated from fermented foods, on a Normal-...

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Autores principales: Garcia-Gonzalez, Natalia, Prete, Roberta, Battista, Natalia, Corsetti, Aldo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6186789/
https://www.ncbi.nlm.nih.gov/pubmed/30349520
http://dx.doi.org/10.3389/fmicb.2018.02392
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author Garcia-Gonzalez, Natalia
Prete, Roberta
Battista, Natalia
Corsetti, Aldo
author_facet Garcia-Gonzalez, Natalia
Prete, Roberta
Battista, Natalia
Corsetti, Aldo
author_sort Garcia-Gonzalez, Natalia
collection PubMed
description Food-associated microbes can reach the gut as viable cells and interact with the human host providing potential health benefits. In this study, we evaluated the impact on cell viability and the adhesion ability of 22 Lactobacillus plantarum strains, mainly isolated from fermented foods, on a Normal-derived Colon Mucosa cell line. Furthermore, due to the presence of mucus layer on the gut epithelium, we also investigated whether the mucin could affect the microbial adhesion property. Our results demonstrated that all the strains displayed a strong ability to adhere to host cells, showing a strain-dependent behavior with preference for cell edges, that resulted not to be affected by the presence of mucin. Based on interleukin-8 release of intestinal cells induced by some Lb. plantarum strains, our data suggest a potential cross-talk with the host immune system as unconventional property of these food-associated microbes.
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spelling pubmed-61867892018-10-22 Adhesion Properties of Food-Associated Lactobacillus plantarum Strains on Human Intestinal Epithelial Cells and Modulation of IL-8 Release Garcia-Gonzalez, Natalia Prete, Roberta Battista, Natalia Corsetti, Aldo Front Microbiol Microbiology Food-associated microbes can reach the gut as viable cells and interact with the human host providing potential health benefits. In this study, we evaluated the impact on cell viability and the adhesion ability of 22 Lactobacillus plantarum strains, mainly isolated from fermented foods, on a Normal-derived Colon Mucosa cell line. Furthermore, due to the presence of mucus layer on the gut epithelium, we also investigated whether the mucin could affect the microbial adhesion property. Our results demonstrated that all the strains displayed a strong ability to adhere to host cells, showing a strain-dependent behavior with preference for cell edges, that resulted not to be affected by the presence of mucin. Based on interleukin-8 release of intestinal cells induced by some Lb. plantarum strains, our data suggest a potential cross-talk with the host immune system as unconventional property of these food-associated microbes. Frontiers Media S.A. 2018-10-08 /pmc/articles/PMC6186789/ /pubmed/30349520 http://dx.doi.org/10.3389/fmicb.2018.02392 Text en Copyright © 2018 Garcia-Gonzalez, Prete, Battista and Corsetti. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Garcia-Gonzalez, Natalia
Prete, Roberta
Battista, Natalia
Corsetti, Aldo
Adhesion Properties of Food-Associated Lactobacillus plantarum Strains on Human Intestinal Epithelial Cells and Modulation of IL-8 Release
title Adhesion Properties of Food-Associated Lactobacillus plantarum Strains on Human Intestinal Epithelial Cells and Modulation of IL-8 Release
title_full Adhesion Properties of Food-Associated Lactobacillus plantarum Strains on Human Intestinal Epithelial Cells and Modulation of IL-8 Release
title_fullStr Adhesion Properties of Food-Associated Lactobacillus plantarum Strains on Human Intestinal Epithelial Cells and Modulation of IL-8 Release
title_full_unstemmed Adhesion Properties of Food-Associated Lactobacillus plantarum Strains on Human Intestinal Epithelial Cells and Modulation of IL-8 Release
title_short Adhesion Properties of Food-Associated Lactobacillus plantarum Strains on Human Intestinal Epithelial Cells and Modulation of IL-8 Release
title_sort adhesion properties of food-associated lactobacillus plantarum strains on human intestinal epithelial cells and modulation of il-8 release
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6186789/
https://www.ncbi.nlm.nih.gov/pubmed/30349520
http://dx.doi.org/10.3389/fmicb.2018.02392
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