Cargando…

Transient Retrovirus-Based CRISPR/Cas9 All-in-One Particles for Efficient, Targeted Gene Knockout

The recently discovered CRISPR/Cas9 system is widely used in basic research and is a useful tool for disease modeling and gene editing therapies. However, long-term expression of DNA-modifying enzymes can be associated with cytotoxicity and is particularly unwanted in clinical gene editing strategie...

Descripción completa

Detalles Bibliográficos
Autores principales:	Knopp, Yvonne, Geis, Franziska K., Heckl, Dirk, Horn, Stefan, Neumann, Thomas, Kuehle, Johannes, Meyer, Janine, Fehse, Boris, Baum, Christopher, Morgan, Michael, Meyer, Johann, Schambach, Axel, Galla, Melanie
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society of Gene & Cell Therapy 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187057/ https://www.ncbi.nlm.nih.gov/pubmed/30317165 http://dx.doi.org/10.1016/j.omtn.2018.09.006

Ejemplares similares

Non-integrating gamma-retroviral vectors as a versatile tool for transient zinc-finger nuclease delivery
por: Bobis-Wozowicz, Sylwia, et al.
Publicado: (2014)

Improved alpharetrovirus-based Gag.MS2 particles for efficient and transient delivery of CRISPR-Cas9 into target cells
por: Baron, Yvonne, et al.
Publicado: (2022)

Deep Characterization and Comparison of Different Retrovirus-like Particles Preloaded with CRISPR/Cas9 RNPs
por: Wichmann, Max, et al.
Publicado: (2023)

Silencing and Transcriptional Regulation of Endogenous Retroviruses: An Overview
por: Geis, Franziska K., et al.
Publicado: (2020)

Correction to: Potent and reversible lentiviral vector restriction in murine induced pluripotent stem cells
por: Geis, Franziska K., et al.
Publicado: (2017)

Potent and reversible lentiviral vector restriction in murine induced pluripotent stem cells
por: Geis, Franziska K., et al.
Publicado: (2017)

CRISPR-Cas9-induced gene knockout in zebrafish
por: Medishetti, Raghavender, et al.
Publicado: (2022)

JACKS: joint analysis of CRISPR/Cas9 knockout screens
por: Allen, Felicity, et al.
Publicado: (2019)

Refined sgRNA efficacy prediction improves large- and small-scale CRISPR–Cas9 applications
por: Labuhn, Maurice, et al.
Publicado: (2018)

LATE–a novel sensitive cell-based assay for the study of CRISPR/Cas9-related long-term adverse treatment effects
por: Głów, Dawid, et al.
Publicado: (2021)

Efficient Gene Knockout in Goats Using CRISPR/Cas9 System
por: Ni, Wei, et al.
Publicado: (2014)

CRISPR/Cas9-mediated gene knockout in the ascidian Ciona intestinalis
por: Sasaki, Haruka, et al.
Publicado: (2014)

User-Friendly Genetic Conditional Knockout Strategies by CRISPR/Cas9
por: Chen, Liangliang, et al.
Publicado: (2018)

CRISPR/Cas9 Methodology for the Generation of Knockout Deletions in Caenorhabditis elegans
por: Au, Vinci, et al.
Publicado: (2018)

CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii
por: Shin, Sung-Eun, et al.
Publicado: (2016)

Consequences of CRISPR-Cas9-Mediated CFTR Knockout in Human Macrophages
por: Zhang, Shuzhong, et al.
Publicado: (2020)

Simplified Gene Knockout by CRISPR-Cas9-Induced Homologous Recombination
por: Dalvie, Neil C., et al.
Publicado: (2021)

CRISPR-Cas knockout of miR21 reduces glioma growth
por: Nieland, Lisa, et al.
Publicado: (2022)

CRISPR/Cas9-mediated gene knockout and interallelic gene conversion in human induced pluripotent stem cells using non-integrative bacteriophage-chimeric retrovirus-like particles
por: Mianné, Joffrey, et al.
Publicado: (2022)

Retroviral gene therapy in Germany with a view on previous experience and future perspectives
por: Morgan, Michael A., et al.
Publicado: (2021)

Prevention of Avian Retrovirus Infection in Chickens Using CRISPR-Cas9 Delivered by Marek’s Disease Virus
por: Li, Kai, et al.
Publicado: (2020)

Synthetic Notch-Receptor-Mediated Transmission of a Transient Signal into Permanent Information via CRISPR/Cas9-Based Genome Editing
por: Sgodda, Malte, et al.
Publicado: (2020)

Efficient gene knockout and genetic interactions: the IN4MER CRISPR/Cas12a multiplex knockout platform
por: Anvar, Nazanin Esmaeili, et al.
Publicado: (2023)

Utilization of proliferable extracellular amastigotes for transient gene expression, drug sensitivity assay, and CRISPR/Cas9-mediated gene knockout in Trypanosoma cruzi
por: Takagi, Yuko, et al.
Publicado: (2019)

One-step generation of knockout pigs by zygote injection of CRISPR/Cas system
por: Hai, Tang, et al.
Publicado: (2014)

Immunoblot screening of CRISPR/Cas9-mediated gene knockouts without selection
por: Estep, Jason A., et al.
Publicado: (2016)

CRISPR-Cas9-Based Knockout of the Prion Protein and Its Effect on the Proteome
por: Mehrabian, Mohadeseh, et al.
Publicado: (2014)

Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency
por: Dang, Ying, et al.
Publicado: (2015)

Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens
por: Hart, Traver, et al.
Publicado: (2017)

Generation of Gene-Knockout Mongolian Gerbils via CRISPR/Cas9 System
por: Wang, Yan, et al.
Publicado: (2020)

Efficient knockout of phytoene desaturase gene using CRISPR/Cas9 in melon
por: Hooghvorst, Isidre, et al.
Publicado: (2019)

An efficient miRNA knockout approach using CRISPR-Cas9 in Xenopus()
por: Godden, Alice M., et al.
Publicado: (2022)

Knockout of phytoene desaturase gene using CRISPR/Cas9 in highbush blueberry
por: Vaia, Giuseppe, et al.
Publicado: (2022)

Generation of gene-of-interest knockouts in murine organoids using CRISPR-Cas9
por: Huber, Anne, et al.
Publicado: (2023)

Impact of CRISPR/Cas9-Mediated CD73 Knockout in Pancreatic Cancer
por: Zhang, Jinping, et al.
Publicado: (2023)

CRISPR/Cas9-mediated gene knockout in the mouse brain using in utero electroporation
por: Shinmyo, Yohei, et al.
Publicado: (2016)

Multigene Knockout Utilizing Off-Target Mutations of the CRISPR/Cas9 System in Rice
por: Endo, Masaki, et al.
Publicado: (2015)

CRISPR/Cas9-mediated generation of a Plac8 knockout mouse model
por: Lee, HyunJeong, et al.
Publicado: (2018)

Streamlined procedure for gene knockouts using all-in-one adenoviral CRISPR-Cas9
por: Jin, Yuan-Hu, et al.
Publicado: (2019)

Validation of microinjection methods for generating knockout mice by CRISPR/Cas-mediated genome engineering
por: Horii, Takuro, et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_ht29ip7usnuffjn11vu49rqtqs